Exon mutations that affect the choice of splice sites used in processing the SV40 late transcripts (original) (raw)
Journal Article
,
McArdle Laboratory for Cancer Research, University of Wisconsin
Madison, WI 53706, USA
1 Present address: Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
Search for other works by this author on:
McArdle Laboratory for Cancer Research, University of Wisconsin
Madison, WI 53706, USA
Search for other works by this author on:
1 Present address: Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
Revision received:
16 July 1985
Published:
12 August 1985
Cite
Madhu B. Somasekhar, Janet E. Mertz, Exon mutations that affect the choice of splice sites used in processing the SV40 late transcripts, Nucleic Acids Research, Volume 13, Issue 15, 12 August 1985, Pages 5591–5609, https://doi.org/10.1093/nar/13.15.5591
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
The spliced species of late SV40 RNAs present in the cytoplasm of cells infected with various wild-type and mutant strains of SV40 that differ in their leader regions were determined using a novel modification of the primer extension method and the S1 nuclease mapping technique. These data indicated that mutations within the first exon of the late RNAs can affect dramatically the utilization of downstream donor and acceptor splice sites. In one instance, a ten base pair insertion within the predominant first exon increased utilization of an infrequently utilized donor splice site such that the small alteration became part of an intervening sequence, thereby suggesting a novel mechanism for regulation of gene expression. In addition, our method enabled detection of a previously unidentified spliced species, representing less than one percentof the SV40 late 19S RNA present in cells infected with wild–type virus, that may be an intermediate in the synthesis of a known doubly spliced 16S RNA species of SV40.
This content is only available as a PDF.
Author notes
1 Present address: Department of Biochemistry, University of Wisconsin, Madison, WI 53706, USA
© IRL Press Limited
I agree to the terms and conditions. You must accept the terms and conditions.
Submit a comment
Name
Affiliations
Comment title
Comment
You have entered an invalid code
Thank you for submitting a comment on this article. Your comment will be reviewed and published at the journal's discretion. Please check for further notifications by email.
Citations
Views
Altmetric
Metrics
Total Views 38
11 Pageviews
27 PDF Downloads
Since 6/1/2017
Month: | Total Views: |
---|---|
June 2017 | 2 |
October 2017 | 1 |
November 2017 | 1 |
December 2017 | 4 |
January 2018 | 1 |
February 2018 | 2 |
March 2018 | 5 |
April 2018 | 4 |
June 2018 | 1 |
July 2018 | 1 |
August 2018 | 1 |
March 2021 | 2 |
August 2022 | 1 |
November 2022 | 1 |
December 2022 | 1 |
December 2023 | 1 |
April 2024 | 4 |
May 2024 | 1 |
June 2024 | 2 |
August 2024 | 1 |
September 2024 | 1 |
Citations
87 Web of Science
×
Email alerts
Citing articles via
More from Oxford Academic