Oat phytochrome A mRNA degradation appears to occur via two distinct pathways. (original) (raw)
Journal Article
,
Department of Botany, Iowa State University, Ames 50011-1020.
Search for other works by this author on:
Department of Botany, Iowa State University, Ames 50011-1020.
Search for other works by this author on:
Navbar Search Filter Mobile Enter search term Search
Abstract
We have identified possible mechanisms for the degradation of oat phytochrome A (PHYA) mRNA. The majority of PHYA mRNA molecules appeared to be degraded prior to removal of the poly(A) tail, a pathway that differs from that reported for the degradation of other eukaryotic mRNAs. Polyadenylated PHYA mRNA contained a pattern of putative degradation products that is consistent with a 5'-->3' exoribonuclease, although the participation of a stochastic endoribonuclease cannot be excluded. The poly(A) tail of PHYA mRNA was heterogeneous in size and ranged from approximately 14 to 220 nucleotides. Early PHYA mRNA degradation events did not appear to involve site-specific endoribonucleases. Approximately 25% of the apparently full-length PHYA mRNA was poly(A) deficient. Oat H4 histone, beta-tubulin, and actin mRNA populations had lower amounts of apparently full-length mRNAs that were poly(A) deficient. Degradation of the poly(A)-deficient PHYA mRNA, a second pathway, appeared to be initiated by a 3'-->5' exoribonucleolytic removal of the poly(A) tail followed by both 5'-->3' and 3'-->5' exoribonuclease activities. Polysome-associated RNA contained putative PHYA mRNA degradation products and was a mixture of polyadenylated and deadenylated PHYA messages, suggesting that the two distinct degradation pathways are polysome associated.
This content is only available as a PDF.
© 1994 by American Society of Plant Biologists
This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open\_access/funder\_policies/chorus/standard\_publication\_model)
Citations
Views
Altmetric
Metrics
Total Views 28
0 Pageviews
28 PDF Downloads
Since 7/1/2021
Month: | Total Views: |
---|---|
July 2021 | 1 |
August 2021 | 3 |
September 2021 | 1 |
October 2021 | 2 |
November 2021 | 2 |
February 2022 | 2 |
March 2022 | 2 |
April 2022 | 1 |
May 2022 | 1 |
August 2022 | 2 |
February 2023 | 1 |
March 2023 | 1 |
April 2023 | 1 |
December 2023 | 1 |
January 2024 | 2 |
March 2024 | 1 |
April 2024 | 1 |
August 2024 | 1 |
September 2024 | 2 |
×
Email alerts
Citing articles via
More from Oxford Academic