Satish Tuteja | AcSIR,INDIA - Academia.edu (original) (raw)

Papers by Satish Tuteja

Research paper thumbnail of A highly efficient 2D exfoliated metal dichalcogenide for the on-farm rapid monitoring of non-esterified fatty acids

Chemical Communications, 2017

Liquid phase exfoliation of 2D MoS2 and bioconjugation with NEFA antibodies to develop a biointer... more Liquid phase exfoliation of 2D MoS2 and bioconjugation with NEFA antibodies to develop a biointerface over the SPE.

Research paper thumbnail of Exploration of two-dimensional bio-functionalized phosphorene nanosheets (black phosphorous) for label free haptoglobin electro-immunosensing applications

Nanotechnology, Feb 14, 2018

We report on the development of an antibody-functionalized interface based on electrochemically a... more We report on the development of an antibody-functionalized interface based on electrochemically active liquid-exfoliated two-dimensional phosphorene (Ph) nanosheets-also known as black phosphorous nanosheets-for the label-free electrochemical immunosensing of a haptoglobin (Hp) biomarker, a clinical marker of severe inflammation. The electrodeposition has been achieved over the screen-printed electrode (SPE) using liquid-assisted ultrasonically exfoliated black phosphorus nanosheets. Subsequently, Ph-SPEs bioconjugated with Hp antibodies (Ab), using electrostatic interactions via a poly-L-lysine linker for biointerface development. Electrochemical analysis demonstrates that the Ab-modified Ph-SPEs (Ab@Ph-SPE) exhibit enhanced electroconducting behavior as compared to the pristine electrodes. This Ab-functionalized phosphorene-based electrochemical immunosensor platform has demonstrated remarkable sensitivity and specificity, having a dynamic linear response range from 0.01-10 mg mlfor Hp in standard and serum samples with a low detection limit (∼0.011 mg ml) using the label-free electrochemical technique. The sensor electrodes were also studied with other closely relative interferents to investigate cross reactivity and specificity. This strategy opens up avenues to POC (point-of-care) and on-farm livestock disease monitoring technologies for multiplexed diagnosis in complex biological samples such as serum. The technique is simple in fabrication and provides an analytical response in less than 60 s.

Research paper thumbnail of Highly sensitive optical biosensing of <i>Staphylococcus aureus</i> with an antibody/metal–organic framework bioconjugate

Analytical Methods, 2019

In this research, a new luminescent bioprobe was developed for the detection of S. aureus based o... more In this research, a new luminescent bioprobe was developed for the detection of S. aureus based on bio-conjugation of an amine functionalized metal–organic framework (NH2-MIL-53(Fe)) with an anti-S. aureus antibody (Ab).

Research paper thumbnail of Recent advancement in biosensors technology for animal and livestock health management

Biosensors and Bioelectronics, Dec 1, 2017

The term biosensors encompasses devices that have the potential to quantify physiological, immuno... more The term biosensors encompasses devices that have the potential to quantify physiological, immunological and behavioural responses of livestock and multiple animal species. Novel biosensing methodologies offer highly specialised monitoring devices for the specific measurement of individual and multiple parameters covering an animal's physiology as well as monitoring of an animal's environment. These devices are not only highly specific and sensitive for the parameters being analysed, but they are also reliable and easy to use, and can accelerate the monitoring process. Novel biosensors in livestock management provide significant benefits and applications in disease detection and isolation, health monitoring and detection of reproductive cycles, as well as monitoring physiological wellbeing of the animal via analysis of the animal's environment. With the development of integrated systems and the Internet of Things, the continuously monitoring devices are expected to become affordable. The data generated from integrated livestock monitoring is anticipated to assist farmers and the agricultural industry to improve animal productivity in the future. The data is expected to reduce the impact of the livestock industry on the environment, while at the same time driving the new wave towards the improvements of viable farming techniques. This review focusses on the emerging technological advancements in monitoring of livestock health for detailed, precise information on productivity, as well as physiology and well-being. Biosensors will contribute to the 4 th revolution in agriculture by incorporating innovative technologies into cost-effective diagnostic methods that can mitigate the potentially catastrophic effects of infectious outbreaks in farmed animals .

Research paper thumbnail of Noninvasive Label-Free Detection of Cortisol and Lactate Using Graphene Embedded Screen-Printed Electrode

Nano-micro Letters, Mar 2, 2018

• The as-electrodeposited 2D nanosheets exhibit enhanced electroconductive nature. • For the firs... more • The as-electrodeposited 2D nanosheets exhibit enhanced electroconductive nature. • For the first time, a dual cortisol and lactate antibody-conjugated graphene biointerface has been developed. The developed assay is label free and applicable for the analysis of clinical samples. • The results are well correlated and validated with existing commercial ELISA kit. The time of detection is very low at B1 min.

Research paper thumbnail of A label-free electrochemical immunosensor for the detection of cardiac marker using graphene quantum dots (GQDs)

Biosensors and Bioelectronics, Dec 1, 2016

A label-free immunosensor based on electrochemical impedance spectroscopy has been developed for ... more A label-free immunosensor based on electrochemical impedance spectroscopy has been developed for the sensitive detection of a cardiac biomarker myoglobin (cMyo). Hydrothermally synthesized graphene quantum dots (GQDs) have been used as an immobilized template on screen printed electrodes for the construction of an impedimetric sensor platform. The GQDs-modified electrode was conjugated with highly specific anti-myoglobin antibodies to develop the desired immunosensor. The values of charge transfer resistance (Rct) were monitored as a function of varying antigen concentration. The Rct value of the immunosensor showed a linear increase (from 0.20 to 0.31kΩ) in the range of 0.01-100ng/mL cMyo. The specific detection of cMyo was also made in the presence of other competing proteins. The limit of detection for the proposed immunosensor was estimated as 0.01ng/mL which is comparable to the standard ELISA techniques.

Research paper thumbnail of Metal-organic frameworks (MOFs) and their composites as electrodes for lithium battery applications: Novel means for alternative energy storage

Coordination Chemistry Reviews, Aug 1, 2019

Metal organic frameworks (MOFs) have emerged as a novel class of advanced functional materials du... more Metal organic frameworks (MOFs) have emerged as a novel class of advanced functional materials due to their many well-known merits (e.g., ultrahigh porosity, favorable pore size distribution, easy functionalization, and structural tailorability). However, their use in various electronics applications is restricted due to their insulating nature. Therefore, a need arises to intercalate MOFs with various functional/conductive materials for synergistic applications like electrode materials (e.g., diverse Li-ion batteries: LIBs). In this review, the recent developments on pristine MOFs and their derived nanostructures (e.g., nanoporous carbons and metal oxides) for electrode applications are discussed in detail. We focused on the recent applications of various MOF-based composites in anodic/cathodic materials (e.g., LIBs, lithium-sulfur batteries (Li-S), lithium-air batteries (Li-O 2), and lithium hybrid electrochemical capacitors (Li-HEC)) along with details associated with their electrochemical performance. This review is expected to help materials scientists to construct a roadmap for the selection of the best possible electrode materials for LIBs.

Research paper thumbnail of Graphene-based multiplexed disposable electrochemical biosensor for rapid on-farm monitoring of NEFA and βHBA dairy biomarkers

Journal of Materials Chemistry B, 2017

Label-free assay using electrodeposited antibody-conjugated graphene biointerface for dual detect... more Label-free assay using electrodeposited antibody-conjugated graphene biointerface for dual detection of NEFA and βHBA from dairy cow blood samples.

Research paper thumbnail of Graphene‐Based Biosensors in Agro‐Defense: Food Safety and Animal Health Diagnosis

Handbook of Graphene, Jun 17, 2019

Research paper thumbnail of Bioconjugation of Different Nanosurfaces With Biorecognition Molecules for the Development of Selective Nanosensor Platforms

Elsevier eBooks, 2019

Abstract With the rising need in the field of point-of-care and on-field diagnostics, various bio... more Abstract With the rising need in the field of point-of-care and on-field diagnostics, various bioreceptor-functionalized nanostructures have been explored for the realization of highly sensitive nano(bio)sensors. Some desirable features of a biosensor include the capability of detection sensitivity in the range of interest, simple sample preprocessing, reproducibility, real-time monitoring of target analytes, and possible regeneration. Antibodies and aptamers (single-stranded DNA or RNA) are the most versatilely used bioreceptors that have been extensively studied and applied successfully for the biosensor development, apart from enzymes, whole cells, and organelles. The response characteristics of nanosensor also depend on the type of nanomaterials embedded on the sensor and the chemistry used for tagging the nanomaterial with the biorecognition element. In this chapter, information has been provided on the functionalization of nanosensor surfaces with biorecognition elements via different conjugation chemistries.

Research paper thumbnail of Liquid exfoliation of 2D MoS<sub>2</sub>nanosheets and their utilization as a label-free electrochemical immunoassay for subclinical ketosis

Research paper thumbnail of Synthesis and Characterization of Silica-Coated Silver Nanoprobe for Paraoxon pesticide Detection

BioNanoScience, Mar 13, 2014

Metal enhanced fluorescence assay-based sensitive detection of a neurotoxic organophosphates pest... more Metal enhanced fluorescence assay-based sensitive detection of a neurotoxic organophosphates pesticide, Paraoxon, is reported. The experimental approach involves the bio-interfacing of organophosphorus hydrolase with a high quantum yield fluorophore, pyranine (8-hydroxyl pyrene-1,3,6-trisulfonic acid trisodium salt), followed by the conjugation of the OPH-pyranine derivative with silica-coated silver nanoparticles (Ag NPs). The above bio-nanoprobe was used for the analysis of the organophosphate pesticide, wherein the induced hydrolysis of the pesticide cause the decrease in pH in the vicinity. An excitation light of 460 nm wavelength was used to monitor the changes in the resulting emission (at 510 nm) with respect to the changing pesticide concentrations (1–100 ppb). The introduction of the silica-coated AgNPs into the nanoprobe system was observed to deliver metal enhanced effect, leading to almost10-fold intensification of the fluorescence signal. The enhanced fluorescence assay format offers linear detection of Paraoxon in the concentration range of 1–100 ng mL−1 with the limit of detection 1 ng mL−1 (1 ppb).

Research paper thumbnail of Graphene‐Based Biosensors in Agro‐Defense: Food Safety and Animal Health Diagnosis

Research paper thumbnail of Bioconjugation of Different Nanosurfaces With Biorecognition Molecules for the Development of Selective Nanosensor Platforms

Advances in Nanosensors for Biological and Environmental Analysis, 2019

Abstract With the rising need in the field of point-of-care and on-field diagnostics, various bio... more Abstract With the rising need in the field of point-of-care and on-field diagnostics, various bioreceptor-functionalized nanostructures have been explored for the realization of highly sensitive nano(bio)sensors. Some desirable features of a biosensor include the capability of detection sensitivity in the range of interest, simple sample preprocessing, reproducibility, real-time monitoring of target analytes, and possible regeneration. Antibodies and aptamers (single-stranded DNA or RNA) are the most versatilely used bioreceptors that have been extensively studied and applied successfully for the biosensor development, apart from enzymes, whole cells, and organelles. The response characteristics of nanosensor also depend on the type of nanomaterials embedded on the sensor and the chemistry used for tagging the nanomaterial with the biorecognition element. In this chapter, information has been provided on the functionalization of nanosensor surfaces with biorecognition elements via different conjugation chemistries.

Research paper thumbnail of Metal-organic frameworks (MOFs) and their composites as electrodes for lithium battery applications: Novel means for alternative energy storage

Coordination Chemistry Reviews, 2019

Metal organic frameworks (MOFs) have emerged as a novel class of advanced functional materials du... more Metal organic frameworks (MOFs) have emerged as a novel class of advanced functional materials due to their many well-known merits (e.g., ultrahigh porosity, favorable pore size distribution, easy functionalization, and structural tailorability). However, their use in various electronics applications is restricted due to their insulating nature. Therefore, a need arises to intercalate MOFs with various functional/conductive materials for synergistic applications like electrode materials (e.g., diverse Li-ion batteries: LIBs). In this review, the recent developments on pristine MOFs and their derived nanostructures (e.g., nanoporous carbons and metal oxides) for electrode applications are discussed in detail. We focused on the recent applications of various MOF-based composites in anodic/cathodic materials (e.g., LIBs, lithium-sulfur batteries (Li-S), lithium-air batteries (Li-O 2), and lithium hybrid electrochemical capacitors (Li-HEC)) along with details associated with their electrochemical performance. This review is expected to help materials scientists to construct a roadmap for the selection of the best possible electrode materials for LIBs.

Research paper thumbnail of Noninvasive Label-Free Detection of Cortisol and Lactate Using Graphene Embedded Screen-Printed Electrode

Nano-Micro Letters, 2018

• The as-electrodeposited 2D nanosheets exhibit enhanced electroconductive nature. • For the firs... more • The as-electrodeposited 2D nanosheets exhibit enhanced electroconductive nature. • For the first time, a dual cortisol and lactate antibody-conjugated graphene biointerface has been developed. The developed assay is label free and applicable for the analysis of clinical samples. • The results are well correlated and validated with existing commercial ELISA kit. The time of detection is very low at B1 min.

Research paper thumbnail of Exploration of two-dimensional bio-functionalized phosphorene nanosheets (black phosphorous) for label free haptoglobin electro-immunosensing applications

Nanotechnology, Jan 14, 2018

We report on the development of an antibody-functionalized interface based on electrochemically a... more We report on the development of an antibody-functionalized interface based on electrochemically active liquid-exfoliated two-dimensional phosphorene (Ph) nanosheets-also known as black phosphorous nanosheets-for the label-free electrochemical immunosensing of a haptoglobin (Hp) biomarker, a clinical marker of severe inflammation. The electrodeposition has been achieved over the screen-printed electrode (SPE) using liquid-assisted ultrasonically exfoliated black phosphorus nanosheets. Subsequently, Ph-SPEs bioconjugated with Hp antibodies (Ab), using electrostatic interactions via a poly-L-lysine linker for biointerface development. Electrochemical analysis demonstrates that the Ab-modified Ph-SPEs (Ab@Ph-SPE) exhibit enhanced electroconducting behavior as compared to the pristine electrodes. This Ab-functionalized phosphorene-based electrochemical immunosensor platform has demonstrated remarkable sensitivity and specificity, having a dynamic linear response range from 0.01-10 mg mlf...

Research paper thumbnail of Liquid exfoliation of 2D MoS2nanosheets and their utilization as a label-free electrochemical immunoassay for subclinical ketosis

Nanoscale, 2017

Schematic illustration of 2D MoS2exfoliation into nanostructures using sonication, and subsequent... more Schematic illustration of 2D MoS2exfoliation into nanostructures using sonication, and subsequent attachment to the working surface of gold modified SPEs by electrodeposition. By virtue of bioconjugation, the MoS2modified SPEs were then functionalised with βHBA antibodies to develop a biointerface.

Research paper thumbnail of Graphene-based multiplexed disposable electrochemical biosensor for rapid on-farm monitoring of NEFA and βHBA dairy biomarkers

Journal of Materials Chemistry B, 2017

Label-free assay using electrodeposited antibody-conjugated graphene biointerface for dual detect... more Label-free assay using electrodeposited antibody-conjugated graphene biointerface for dual detection of NEFA and βHBA from dairy cow blood samples.

Research paper thumbnail of A highly efficient 2D exfoliated metal dichalcogenide for the on-farm rapid monitoring of non-esterified fatty acids

Chem. Commun., 2017

Liquid phase exfoliation of 2D MoS2 and bioconjugation with NEFA antibodies to develop a biointer... more Liquid phase exfoliation of 2D MoS2 and bioconjugation with NEFA antibodies to develop a biointerface over the SPE.

Research paper thumbnail of A highly efficient 2D exfoliated metal dichalcogenide for the on-farm rapid monitoring of non-esterified fatty acids

Chemical Communications, 2017

Liquid phase exfoliation of 2D MoS2 and bioconjugation with NEFA antibodies to develop a biointer... more Liquid phase exfoliation of 2D MoS2 and bioconjugation with NEFA antibodies to develop a biointerface over the SPE.

Research paper thumbnail of Exploration of two-dimensional bio-functionalized phosphorene nanosheets (black phosphorous) for label free haptoglobin electro-immunosensing applications

Nanotechnology, Feb 14, 2018

We report on the development of an antibody-functionalized interface based on electrochemically a... more We report on the development of an antibody-functionalized interface based on electrochemically active liquid-exfoliated two-dimensional phosphorene (Ph) nanosheets-also known as black phosphorous nanosheets-for the label-free electrochemical immunosensing of a haptoglobin (Hp) biomarker, a clinical marker of severe inflammation. The electrodeposition has been achieved over the screen-printed electrode (SPE) using liquid-assisted ultrasonically exfoliated black phosphorus nanosheets. Subsequently, Ph-SPEs bioconjugated with Hp antibodies (Ab), using electrostatic interactions via a poly-L-lysine linker for biointerface development. Electrochemical analysis demonstrates that the Ab-modified Ph-SPEs (Ab@Ph-SPE) exhibit enhanced electroconducting behavior as compared to the pristine electrodes. This Ab-functionalized phosphorene-based electrochemical immunosensor platform has demonstrated remarkable sensitivity and specificity, having a dynamic linear response range from 0.01-10 mg mlfor Hp in standard and serum samples with a low detection limit (∼0.011 mg ml) using the label-free electrochemical technique. The sensor electrodes were also studied with other closely relative interferents to investigate cross reactivity and specificity. This strategy opens up avenues to POC (point-of-care) and on-farm livestock disease monitoring technologies for multiplexed diagnosis in complex biological samples such as serum. The technique is simple in fabrication and provides an analytical response in less than 60 s.

Research paper thumbnail of Highly sensitive optical biosensing of <i>Staphylococcus aureus</i> with an antibody/metal–organic framework bioconjugate

Analytical Methods, 2019

In this research, a new luminescent bioprobe was developed for the detection of S. aureus based o... more In this research, a new luminescent bioprobe was developed for the detection of S. aureus based on bio-conjugation of an amine functionalized metal–organic framework (NH2-MIL-53(Fe)) with an anti-S. aureus antibody (Ab).

Research paper thumbnail of Recent advancement in biosensors technology for animal and livestock health management

Biosensors and Bioelectronics, Dec 1, 2017

The term biosensors encompasses devices that have the potential to quantify physiological, immuno... more The term biosensors encompasses devices that have the potential to quantify physiological, immunological and behavioural responses of livestock and multiple animal species. Novel biosensing methodologies offer highly specialised monitoring devices for the specific measurement of individual and multiple parameters covering an animal's physiology as well as monitoring of an animal's environment. These devices are not only highly specific and sensitive for the parameters being analysed, but they are also reliable and easy to use, and can accelerate the monitoring process. Novel biosensors in livestock management provide significant benefits and applications in disease detection and isolation, health monitoring and detection of reproductive cycles, as well as monitoring physiological wellbeing of the animal via analysis of the animal's environment. With the development of integrated systems and the Internet of Things, the continuously monitoring devices are expected to become affordable. The data generated from integrated livestock monitoring is anticipated to assist farmers and the agricultural industry to improve animal productivity in the future. The data is expected to reduce the impact of the livestock industry on the environment, while at the same time driving the new wave towards the improvements of viable farming techniques. This review focusses on the emerging technological advancements in monitoring of livestock health for detailed, precise information on productivity, as well as physiology and well-being. Biosensors will contribute to the 4 th revolution in agriculture by incorporating innovative technologies into cost-effective diagnostic methods that can mitigate the potentially catastrophic effects of infectious outbreaks in farmed animals .

Research paper thumbnail of Noninvasive Label-Free Detection of Cortisol and Lactate Using Graphene Embedded Screen-Printed Electrode

Nano-micro Letters, Mar 2, 2018

• The as-electrodeposited 2D nanosheets exhibit enhanced electroconductive nature. • For the firs... more • The as-electrodeposited 2D nanosheets exhibit enhanced electroconductive nature. • For the first time, a dual cortisol and lactate antibody-conjugated graphene biointerface has been developed. The developed assay is label free and applicable for the analysis of clinical samples. • The results are well correlated and validated with existing commercial ELISA kit. The time of detection is very low at B1 min.

Research paper thumbnail of A label-free electrochemical immunosensor for the detection of cardiac marker using graphene quantum dots (GQDs)

Biosensors and Bioelectronics, Dec 1, 2016

A label-free immunosensor based on electrochemical impedance spectroscopy has been developed for ... more A label-free immunosensor based on electrochemical impedance spectroscopy has been developed for the sensitive detection of a cardiac biomarker myoglobin (cMyo). Hydrothermally synthesized graphene quantum dots (GQDs) have been used as an immobilized template on screen printed electrodes for the construction of an impedimetric sensor platform. The GQDs-modified electrode was conjugated with highly specific anti-myoglobin antibodies to develop the desired immunosensor. The values of charge transfer resistance (Rct) were monitored as a function of varying antigen concentration. The Rct value of the immunosensor showed a linear increase (from 0.20 to 0.31kΩ) in the range of 0.01-100ng/mL cMyo. The specific detection of cMyo was also made in the presence of other competing proteins. The limit of detection for the proposed immunosensor was estimated as 0.01ng/mL which is comparable to the standard ELISA techniques.

Research paper thumbnail of Metal-organic frameworks (MOFs) and their composites as electrodes for lithium battery applications: Novel means for alternative energy storage

Coordination Chemistry Reviews, Aug 1, 2019

Metal organic frameworks (MOFs) have emerged as a novel class of advanced functional materials du... more Metal organic frameworks (MOFs) have emerged as a novel class of advanced functional materials due to their many well-known merits (e.g., ultrahigh porosity, favorable pore size distribution, easy functionalization, and structural tailorability). However, their use in various electronics applications is restricted due to their insulating nature. Therefore, a need arises to intercalate MOFs with various functional/conductive materials for synergistic applications like electrode materials (e.g., diverse Li-ion batteries: LIBs). In this review, the recent developments on pristine MOFs and their derived nanostructures (e.g., nanoporous carbons and metal oxides) for electrode applications are discussed in detail. We focused on the recent applications of various MOF-based composites in anodic/cathodic materials (e.g., LIBs, lithium-sulfur batteries (Li-S), lithium-air batteries (Li-O 2), and lithium hybrid electrochemical capacitors (Li-HEC)) along with details associated with their electrochemical performance. This review is expected to help materials scientists to construct a roadmap for the selection of the best possible electrode materials for LIBs.

Research paper thumbnail of Graphene-based multiplexed disposable electrochemical biosensor for rapid on-farm monitoring of NEFA and βHBA dairy biomarkers

Journal of Materials Chemistry B, 2017

Label-free assay using electrodeposited antibody-conjugated graphene biointerface for dual detect... more Label-free assay using electrodeposited antibody-conjugated graphene biointerface for dual detection of NEFA and βHBA from dairy cow blood samples.

Research paper thumbnail of Graphene‐Based Biosensors in Agro‐Defense: Food Safety and Animal Health Diagnosis

Handbook of Graphene, Jun 17, 2019

Research paper thumbnail of Bioconjugation of Different Nanosurfaces With Biorecognition Molecules for the Development of Selective Nanosensor Platforms

Elsevier eBooks, 2019

Abstract With the rising need in the field of point-of-care and on-field diagnostics, various bio... more Abstract With the rising need in the field of point-of-care and on-field diagnostics, various bioreceptor-functionalized nanostructures have been explored for the realization of highly sensitive nano(bio)sensors. Some desirable features of a biosensor include the capability of detection sensitivity in the range of interest, simple sample preprocessing, reproducibility, real-time monitoring of target analytes, and possible regeneration. Antibodies and aptamers (single-stranded DNA or RNA) are the most versatilely used bioreceptors that have been extensively studied and applied successfully for the biosensor development, apart from enzymes, whole cells, and organelles. The response characteristics of nanosensor also depend on the type of nanomaterials embedded on the sensor and the chemistry used for tagging the nanomaterial with the biorecognition element. In this chapter, information has been provided on the functionalization of nanosensor surfaces with biorecognition elements via different conjugation chemistries.

Research paper thumbnail of Liquid exfoliation of 2D MoS<sub>2</sub>nanosheets and their utilization as a label-free electrochemical immunoassay for subclinical ketosis

Research paper thumbnail of Synthesis and Characterization of Silica-Coated Silver Nanoprobe for Paraoxon pesticide Detection

BioNanoScience, Mar 13, 2014

Metal enhanced fluorescence assay-based sensitive detection of a neurotoxic organophosphates pest... more Metal enhanced fluorescence assay-based sensitive detection of a neurotoxic organophosphates pesticide, Paraoxon, is reported. The experimental approach involves the bio-interfacing of organophosphorus hydrolase with a high quantum yield fluorophore, pyranine (8-hydroxyl pyrene-1,3,6-trisulfonic acid trisodium salt), followed by the conjugation of the OPH-pyranine derivative with silica-coated silver nanoparticles (Ag NPs). The above bio-nanoprobe was used for the analysis of the organophosphate pesticide, wherein the induced hydrolysis of the pesticide cause the decrease in pH in the vicinity. An excitation light of 460 nm wavelength was used to monitor the changes in the resulting emission (at 510 nm) with respect to the changing pesticide concentrations (1–100 ppb). The introduction of the silica-coated AgNPs into the nanoprobe system was observed to deliver metal enhanced effect, leading to almost10-fold intensification of the fluorescence signal. The enhanced fluorescence assay format offers linear detection of Paraoxon in the concentration range of 1–100 ng mL−1 with the limit of detection 1 ng mL−1 (1 ppb).

Research paper thumbnail of Graphene‐Based Biosensors in Agro‐Defense: Food Safety and Animal Health Diagnosis

Research paper thumbnail of Bioconjugation of Different Nanosurfaces With Biorecognition Molecules for the Development of Selective Nanosensor Platforms

Advances in Nanosensors for Biological and Environmental Analysis, 2019

Abstract With the rising need in the field of point-of-care and on-field diagnostics, various bio... more Abstract With the rising need in the field of point-of-care and on-field diagnostics, various bioreceptor-functionalized nanostructures have been explored for the realization of highly sensitive nano(bio)sensors. Some desirable features of a biosensor include the capability of detection sensitivity in the range of interest, simple sample preprocessing, reproducibility, real-time monitoring of target analytes, and possible regeneration. Antibodies and aptamers (single-stranded DNA or RNA) are the most versatilely used bioreceptors that have been extensively studied and applied successfully for the biosensor development, apart from enzymes, whole cells, and organelles. The response characteristics of nanosensor also depend on the type of nanomaterials embedded on the sensor and the chemistry used for tagging the nanomaterial with the biorecognition element. In this chapter, information has been provided on the functionalization of nanosensor surfaces with biorecognition elements via different conjugation chemistries.

Research paper thumbnail of Metal-organic frameworks (MOFs) and their composites as electrodes for lithium battery applications: Novel means for alternative energy storage

Coordination Chemistry Reviews, 2019

Metal organic frameworks (MOFs) have emerged as a novel class of advanced functional materials du... more Metal organic frameworks (MOFs) have emerged as a novel class of advanced functional materials due to their many well-known merits (e.g., ultrahigh porosity, favorable pore size distribution, easy functionalization, and structural tailorability). However, their use in various electronics applications is restricted due to their insulating nature. Therefore, a need arises to intercalate MOFs with various functional/conductive materials for synergistic applications like electrode materials (e.g., diverse Li-ion batteries: LIBs). In this review, the recent developments on pristine MOFs and their derived nanostructures (e.g., nanoporous carbons and metal oxides) for electrode applications are discussed in detail. We focused on the recent applications of various MOF-based composites in anodic/cathodic materials (e.g., LIBs, lithium-sulfur batteries (Li-S), lithium-air batteries (Li-O 2), and lithium hybrid electrochemical capacitors (Li-HEC)) along with details associated with their electrochemical performance. This review is expected to help materials scientists to construct a roadmap for the selection of the best possible electrode materials for LIBs.

Research paper thumbnail of Noninvasive Label-Free Detection of Cortisol and Lactate Using Graphene Embedded Screen-Printed Electrode

Nano-Micro Letters, 2018

• The as-electrodeposited 2D nanosheets exhibit enhanced electroconductive nature. • For the firs... more • The as-electrodeposited 2D nanosheets exhibit enhanced electroconductive nature. • For the first time, a dual cortisol and lactate antibody-conjugated graphene biointerface has been developed. The developed assay is label free and applicable for the analysis of clinical samples. • The results are well correlated and validated with existing commercial ELISA kit. The time of detection is very low at B1 min.

Research paper thumbnail of Exploration of two-dimensional bio-functionalized phosphorene nanosheets (black phosphorous) for label free haptoglobin electro-immunosensing applications

Nanotechnology, Jan 14, 2018

We report on the development of an antibody-functionalized interface based on electrochemically a... more We report on the development of an antibody-functionalized interface based on electrochemically active liquid-exfoliated two-dimensional phosphorene (Ph) nanosheets-also known as black phosphorous nanosheets-for the label-free electrochemical immunosensing of a haptoglobin (Hp) biomarker, a clinical marker of severe inflammation. The electrodeposition has been achieved over the screen-printed electrode (SPE) using liquid-assisted ultrasonically exfoliated black phosphorus nanosheets. Subsequently, Ph-SPEs bioconjugated with Hp antibodies (Ab), using electrostatic interactions via a poly-L-lysine linker for biointerface development. Electrochemical analysis demonstrates that the Ab-modified Ph-SPEs (Ab@Ph-SPE) exhibit enhanced electroconducting behavior as compared to the pristine electrodes. This Ab-functionalized phosphorene-based electrochemical immunosensor platform has demonstrated remarkable sensitivity and specificity, having a dynamic linear response range from 0.01-10 mg mlf...

Research paper thumbnail of Liquid exfoliation of 2D MoS2nanosheets and their utilization as a label-free electrochemical immunoassay for subclinical ketosis

Nanoscale, 2017

Schematic illustration of 2D MoS2exfoliation into nanostructures using sonication, and subsequent... more Schematic illustration of 2D MoS2exfoliation into nanostructures using sonication, and subsequent attachment to the working surface of gold modified SPEs by electrodeposition. By virtue of bioconjugation, the MoS2modified SPEs were then functionalised with βHBA antibodies to develop a biointerface.

Research paper thumbnail of Graphene-based multiplexed disposable electrochemical biosensor for rapid on-farm monitoring of NEFA and βHBA dairy biomarkers

Journal of Materials Chemistry B, 2017

Label-free assay using electrodeposited antibody-conjugated graphene biointerface for dual detect... more Label-free assay using electrodeposited antibody-conjugated graphene biointerface for dual detection of NEFA and βHBA from dairy cow blood samples.

Research paper thumbnail of A highly efficient 2D exfoliated metal dichalcogenide for the on-farm rapid monitoring of non-esterified fatty acids

Chem. Commun., 2017

Liquid phase exfoliation of 2D MoS2 and bioconjugation with NEFA antibodies to develop a biointer... more Liquid phase exfoliation of 2D MoS2 and bioconjugation with NEFA antibodies to develop a biointerface over the SPE.