A dynamic knockout reveals that conformational fluctuations influence the chemical step of enzyme catalysis. (original) (raw)
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- Stephen Benkovic
- Bhabha et al
Bhabha G, Lee J, Ekiert DC, Gam J, Wilson IA, Dyson HJ, Benkovic SJ, Wright PE
Science. 2011 Apr 8;332(6026):234-8. doi: 10.1126/science.1198542. (Link opens in a new window) PubMed (Link opens in a new window) Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110828 | pET27b-ecDHFR-N23PP | Bacterial expression of E.coli DHFR N23PP mutant |
| 110829 | pET27b-ecDHFR-N23PP/S148A | Bacterial expression of E.coli DHFR N23PP/S148A mutant |