Birhan M Ejigu | Addis Ababa University (original) (raw)
Papers by Birhan M Ejigu
Background: Oropharyngeal Candidiasis (OPC) is the most frequent opportunistic fungal infection o... more Background: Oropharyngeal Candidiasis (OPC) is the most frequent opportunistic fungal infection of the oral cavity in HIV infected patients. OPC is associated with increased morbidity and mortality in immunosuppressed patients. However, data on Species distribution and antifungal susceptibility profiles of yeast isolated from HIV-infected patients in Ethiopia is limited. Objective: The aim of the study was to determine the spectrum of Oropharyngeal yeast isolates from HIV patients and to evaluate antifungal drug susceptibility profile of yeast isolates to commonly used antifungal drugs. Method: A cross sectional study was conducted in Zewditu Memorial Hospital, Addis Ababa. Oral swabs were collected from 224 HIV patients and cultured on Sabouraud Dextrose Agar with chloramphenicol. All the isolates were characterized to a species level following standard microbiological techniques. The antifungal susceptibility profiles of all yeast were determined using disk diffusion method. Resul...
Background: Oropharyngeal Candidiasis (OPC) is the most frequent opportunistic fungal infection o... more Background: Oropharyngeal Candidiasis (OPC) is the most frequent opportunistic fungal infection of the oral cavity in HIV infected patients. OPC is associated with increased morbidity and mortality in immunosuppressed patients. However, data on Species distribution and antifungal susceptibility profiles of yeast isolated from HIV-infected patients in Ethiopia is limited. Objective: The aim of the study was to determine the spectrum of Oropharyngeal yeast isolates from HIV patients and to evaluate antifungal drug susceptibility profile of yeast isolates to commonly used antifungal drugs. Method: A cross sectional study was conducted in Zewditu Memorial Hospital, Addis Ababa. Oral swabs were collected from 224 HIV patients and cultured on Sabouraud Dextrose Agar with chloramphenicol. All the isolates were characterized to a species level following standard microbiological techniques. The antifungal susceptibility profiles of all yeast were determined using disk diffusion method. Result: Of 224 HIV patients screened, 139 (62.1%) were colonized by yeasts. The rate of colonization is higher in patients that non HAART than HAART initiated (72.3% versus 51.8%). A total of 155 yeasts were isolated of which 153 (98.7%) were accounted by Candida consisting of five species and 2(1.3%) non-candida yeasts. Out of all isolates C. albicans was the most frequently isolated species accounting 68.4% followed by C. glabrata (15.5%) and C. tropicalis (11%). The Antifungal susceptibility pattern of the yeast isolates for Fluconazole, ketoconazole, Amphotericin B, Clotrimazole, Nystatin and Miconazole showed a resistance of 12.3%, 8.4%, 5.8%, 2.6%, 1.3%, and 0.6% respectively. C. albicans were 5.7% resistant to ketoconazole and 6.6% resistant to Fluconazole. C. glabrata and C. tropicalis were 12.5% and 17.6% resistant to ketoconazole and 16.6% and 35.3% resistant to fluconazole respectively. viii Conclusion: The spectrum of yeast isolated in the present study was relatively high even if C. albicans was the predominantly isolated yeast. The results of this study also revealed that development of drug resistance by yeast isolates were considerable. Isolation, characterization and evaluating drug susceptibility pattern of the isolates in this hospital in particular and in health institutions through out of the country in general is recommended. Annexes Annex I. English Versions of Participant Information Sheet My name is BIRHAN MOGES. I am a laboratory technologist postgraduate student at Addis Ababa University. Now I am conducting a study entitled Species distribution and in vitro antifungal susceptibility of Oropharyngeal yeast isolates from HIV patients in
International Journal of Microbiology, 2016
Background.In Ethiopia, little is known regarding the distribution and thein vitroantifungal susc... more Background.In Ethiopia, little is known regarding the distribution and thein vitroantifungal susceptibility profile of yeasts.Objective.This study was undertaken to determine the spectrum and thein vitroantifungal susceptibility pattern of yeasts isolated from HIV infected patients with OPC.Method.Oral pharyngeal swabs taken from oral lesions of study subjects were inoculated onto Sabouraud Dextrose Agar. Yeasts were identified by employing conventional test procedures and the susceptibility of yeasts to antifungal agents was evaluated by disk diffusion assay method.Result.One hundred and fifty-five yeast isolates were recovered of which 91 isolates were from patients that were not under HAART and 64 were from patients that were under HAART.C. albicanswas the most frequently isolated species followed byC. glabrata, C. tropicalis, C. krusei, C. kefyr, Cryptococcus laurentii, and Rhodotorulaspecies. Irrespective of yeasts isolated and identified, 5.8%, 5.8%, 12.3%, 8.4%, 0.6%, and 1.3...
BMC Health Services Research
Background Knowing customers’ level of satisfaction is relevant to improve and provide quality he... more Background Knowing customers’ level of satisfaction is relevant to improve and provide quality health care services. In the clinical laboratory, monitoring customers’ satisfaction is an important indicator of the quality management system and required by international laboratory standards. However, in Ethiopia, there has not been baseline data about the satisfaction level of patients’ with laboratory services at the national level. The aim of this national level survey was to assess patients’ satisfaction level with laboratory services at public hospitals in Ethiopia. Methods A national survey was conducted using an institutional based cross-sectional study design was employed from 01 to 30 November 2017. A total of 2399 patients were selected randomly from 60 public hospitals. Data was collected using structured questionnaire, entered in Epi Info and analyzed with SPSS software. Multiple logistic regression model was fitted to identify predictors of patients’ satisfaction with labo...
Background: Oropharyngeal Candidiasis (OPC) is the most frequent opportunistic fungal infection o... more Background: Oropharyngeal Candidiasis (OPC) is the most frequent opportunistic fungal infection of the oral cavity in HIV infected patients. OPC is associated with increased morbidity and mortality in immunosuppressed patients. However, data on Species distribution and antifungal susceptibility profiles of yeast isolated from HIV-infected patients in Ethiopia is limited. Objective: The aim of the study was to determine the spectrum of Oropharyngeal yeast isolates from HIV patients and to evaluate antifungal drug susceptibility profile of yeast isolates to commonly used antifungal drugs. Method: A cross sectional study was conducted in Zewditu Memorial Hospital, Addis Ababa. Oral swabs were collected from 224 HIV patients and cultured on Sabouraud Dextrose Agar with chloramphenicol. All the isolates were characterized to a species level following standard microbiological techniques. The antifungal susceptibility profiles of all yeast were determined using disk diffusion method. Resul...
Background: Oropharyngeal Candidiasis (OPC) is the most frequent opportunistic fungal infection o... more Background: Oropharyngeal Candidiasis (OPC) is the most frequent opportunistic fungal infection of the oral cavity in HIV infected patients. OPC is associated with increased morbidity and mortality in immunosuppressed patients. However, data on Species distribution and antifungal susceptibility profiles of yeast isolated from HIV-infected patients in Ethiopia is limited. Objective: The aim of the study was to determine the spectrum of Oropharyngeal yeast isolates from HIV patients and to evaluate antifungal drug susceptibility profile of yeast isolates to commonly used antifungal drugs. Method: A cross sectional study was conducted in Zewditu Memorial Hospital, Addis Ababa. Oral swabs were collected from 224 HIV patients and cultured on Sabouraud Dextrose Agar with chloramphenicol. All the isolates were characterized to a species level following standard microbiological techniques. The antifungal susceptibility profiles of all yeast were determined using disk diffusion method. Result: Of 224 HIV patients screened, 139 (62.1%) were colonized by yeasts. The rate of colonization is higher in patients that non HAART than HAART initiated (72.3% versus 51.8%). A total of 155 yeasts were isolated of which 153 (98.7%) were accounted by Candida consisting of five species and 2(1.3%) non-candida yeasts. Out of all isolates C. albicans was the most frequently isolated species accounting 68.4% followed by C. glabrata (15.5%) and C. tropicalis (11%). The Antifungal susceptibility pattern of the yeast isolates for Fluconazole, ketoconazole, Amphotericin B, Clotrimazole, Nystatin and Miconazole showed a resistance of 12.3%, 8.4%, 5.8%, 2.6%, 1.3%, and 0.6% respectively. C. albicans were 5.7% resistant to ketoconazole and 6.6% resistant to Fluconazole. C. glabrata and C. tropicalis were 12.5% and 17.6% resistant to ketoconazole and 16.6% and 35.3% resistant to fluconazole respectively. viii Conclusion: The spectrum of yeast isolated in the present study was relatively high even if C. albicans was the predominantly isolated yeast. The results of this study also revealed that development of drug resistance by yeast isolates were considerable. Isolation, characterization and evaluating drug susceptibility pattern of the isolates in this hospital in particular and in health institutions through out of the country in general is recommended. Annexes Annex I. English Versions of Participant Information Sheet My name is BIRHAN MOGES. I am a laboratory technologist postgraduate student at Addis Ababa University. Now I am conducting a study entitled Species distribution and in vitro antifungal susceptibility of Oropharyngeal yeast isolates from HIV patients in
International Journal of Microbiology, 2016
Background.In Ethiopia, little is known regarding the distribution and thein vitroantifungal susc... more Background.In Ethiopia, little is known regarding the distribution and thein vitroantifungal susceptibility profile of yeasts.Objective.This study was undertaken to determine the spectrum and thein vitroantifungal susceptibility pattern of yeasts isolated from HIV infected patients with OPC.Method.Oral pharyngeal swabs taken from oral lesions of study subjects were inoculated onto Sabouraud Dextrose Agar. Yeasts were identified by employing conventional test procedures and the susceptibility of yeasts to antifungal agents was evaluated by disk diffusion assay method.Result.One hundred and fifty-five yeast isolates were recovered of which 91 isolates were from patients that were not under HAART and 64 were from patients that were under HAART.C. albicanswas the most frequently isolated species followed byC. glabrata, C. tropicalis, C. krusei, C. kefyr, Cryptococcus laurentii, and Rhodotorulaspecies. Irrespective of yeasts isolated and identified, 5.8%, 5.8%, 12.3%, 8.4%, 0.6%, and 1.3...
BMC Health Services Research
Background Knowing customers’ level of satisfaction is relevant to improve and provide quality he... more Background Knowing customers’ level of satisfaction is relevant to improve and provide quality health care services. In the clinical laboratory, monitoring customers’ satisfaction is an important indicator of the quality management system and required by international laboratory standards. However, in Ethiopia, there has not been baseline data about the satisfaction level of patients’ with laboratory services at the national level. The aim of this national level survey was to assess patients’ satisfaction level with laboratory services at public hospitals in Ethiopia. Methods A national survey was conducted using an institutional based cross-sectional study design was employed from 01 to 30 November 2017. A total of 2399 patients were selected randomly from 60 public hospitals. Data was collected using structured questionnaire, entered in Epi Info and analyzed with SPSS software. Multiple logistic regression model was fitted to identify predictors of patients’ satisfaction with labo...