Sheikh Ahmed | Gcuf - Academia.edu (original) (raw)
Papers by Sheikh Ahmed
International Journal of Materials Engineering Innovation
Turkish Journal of Agriculture and Forestry
This paper examines wood anatomy as it relates to safranine solution penetration in Samanea saman... more This paper examines wood anatomy as it relates to safranine solution penetration in Samanea saman, which belongs to the family Mimosoideae and is native to Bangladesh. The wood of this species was characterized by diffuse-porous vessels with simple perforations, alternate and opposite vestured intervessel pits, gums in heartwood vessels, vascicentric paratracheal axial parenchyma, lozenge-aliform, confluent prismatic crystals in fiber, and chambered axial parenchyma cells. Different anatomical features affect longitudinal and lateral liquid permeability. Herein microstructures that controlled liquid penetration are described and compared. We observed that ray cell lumen diameter, end-wall pit number and diameter, ray cell length, vessel number, and both vessel and fiber diameter and length affected the depth of liquid flow. Safranine solution formed a meniscus while traveling through different types of cells. Air trapped in capillaries blocked safranine solution penetration. Following a go-stop-go cycle, the speed of penetration slowly decreased over time.
The American Journal of Tropical Medicine and Hygiene
ABSTRACTThere are an estimated 2,000 children with β-thalassemia in the province Baluchistan of P... more ABSTRACTThere are an estimated 2,000 children with β-thalassemia in the province Baluchistan of Pakistan. These children are at high risk of acquiring transfusion-transmitted infections (TTIs) due to their need of regular blood transfusions for survival. Therefore, we investigated the frequencies of TTIs among these multi-transfused patients in a region where the WHO guidelines for blood safety are not always followed. Sera from 400 children (mean age 7.7 ± 4.70 years) treated at two thalassemia centers in Baluchistan were investigated for TTIs. Eleven (2.8%) were hepatitis B surface antigen positive, and 72 (18.3%) had anti-hepatitis C virus (HCV), two of which were infected with both viruses. Only 22% of the children had been reached by the program for universal hepatitis B virus (HBV) vaccination which started in 2004. Half (51%) of the HCV infected had also been HBV infected. The HBV- and HCV-infected patients were older and had received more blood transfusions than the uninfect...
In most epithelia, desmosomes are important adhesion structures which have a key role in linking ... more In most epithelia, desmosomes are important adhesion structures which have a key role in linking the intermediate filament network of one cell to its neighbor, thus forming a strong bond. These junctions are essential for retaining the integrity of organs, subject to mechanical stress, particularly the heart and skin. Desmogleins and desmocollins belong to super family of cadherin, which mediate adhesion at desmosomes. Through a series of protein interactions, desmosomal cadherin tails get associated with Cytoplasmic components of the desmosome, the sites of desmosome assemblage are employed with intermediate filaments. There are four types of desmogleins (Dsg1, Dsg2, Dsg3 and Dsg4) and three types of desmocollins (Dsc 1, Dsc 2, and Dsc 3). These types of desmosomal cadherins are involved in various defects. This review outlines the structure, function and associated diseases of cadherin superfamily. I believe that this review will assist researchers to enhance their basic knowledge...
It is estimated that around more than 2% of the population of world is suffering with hepatitis C... more It is estimated that around more than 2% of the population of world is suffering with hepatitis C virus and death cases because of cirrhosis and liver cancer is more than 350000.The load and epidemiology of HCV infection is different in the different parts of the world. It vary with country particular prevalence range from 10%. In comparison with USA and other urbanized countries the spread of infection in poor countries commonly outcome of contact to infected blood in society settings and healthcare centers. HCV management programs must be country specific which may be different by background and rank of economic progress. Worldwide HCV is a main cause of liver disease and in future it would be a major cause of morbidity and mortality. The complication related to the geographic allocation of HCV infection and its linked risk factor increases its progression. No vaccine is available for HCV and no later than exposure for HCV prophylaxis, so the focal point of most important escaping...
Variability in power systems is increasing due to pushing the system to limits for economic purpo... more Variability in power systems is increasing due to pushing the system to limits for economic purposes, the inclusion of new energy sources like wind turbines and photovoltaic, and the introduction of new types of loads such as electric vehicle chargers. In this new environment, system monitoring and control must keep pace to insure system stability and reliability on a wide area scale. Phasor measurement unit technology implementation is growing and can be used to provide input signals to new types of control. Fuzzy logic based power system stabilizer (PSS)
The FASEB Journal, 2016
BackgroundNon-exclusive breastfeeding and poor environmental hygiene in early infancy may perturb... more BackgroundNon-exclusive breastfeeding and poor environmental hygiene in early infancy may perturb the gut microbiota to cause local inflammation and systemic exposure to bacterial lipopolysaccharid...
Journal of emerging technologies and innovative research, 2017
PFA copy of full length research paper for possible publication in your respected journal. Kindly... more PFA copy of full length research paper for possible publication in your respected journal. Kindly acknowledge. Regards 1.Dr. Suheela chisti 2. irfan ahmed sheikh
Algorithms, 2021
Automatic Number Plate Recognition (ANPR) has been widely used in different domains, such as car ... more Automatic Number Plate Recognition (ANPR) has been widely used in different domains, such as car park management, traffic management, tolling, and intelligent transport systems. Despite this technology’s importance, the existing ANPR approaches suffer from the accurate identification of number plats due to its different size, orientation, and shapes across different regions worldwide. In this paper, we are studying these challenges by implementing a case study for smart car towing management using Machine Learning (ML) models. The developed mobile-based system uses different approaches and techniques to enhance the accuracy of recognizing number plates in real-time. First, we developed an algorithm to accurately detect the number plate’s location on the car body. Then, the bounding box of the plat is extracted and converted into a grayscale image. Second, we applied a series of filters to detect the alphanumeric characters’ contours within the grayscale image. Third, the detected th...
International Journal of Livestock Research, 2021
Journal of Advanced Biotechnology and Experimental Therapeutics, 2019
ABSTRACT: The experiments were conducted for grading unknown agarwood, to analyze the total pheno... more ABSTRACT: The experiments were conducted for grading unknown agarwood, to analyze the total phenolics, total flavonoids, antioxidant status of agarwood and its crude oil and also to identify the existing bacteria that assist in the fermentation of agarwood during soaking period. Grades of unknown agarwoods were determined based on density metric method and cross checked for ether extract. Total phenolics of agar wood and oils were analyzed through Folin-Ciocalteu method and total flavonoid contents of agar oils were estimated using Aluminium chloride colorimetric method. For the determination of antioxidant status of agarwood oil, the inhibitory effects of oil extracts on DPPH radical was measured. To identify the bacterial genus, several biochemical tests were conducted. Results showed that insect infested agarwood was classified as Grade-1 agarwood (Calculated density was 0.641 g/cm 3) and contained the highest amount ether extract of 18.90 ± 0.60%, total phenolic content of 3.5 ± 0.06 (mg GAE/g) and flavonoid content of 7.82 ± 0.23 (µg QE/ml). IC 50 values obtained by DPPH activity for Aquillaria malaccensis oil extract was found to be 0.904 µg/ml. Bacillus spp. was recognized as the mostly prevalent bacteria responsible for fermentation of agarwood which exhibit special role in increasing the yield of agar oil. If agarwood and agar oil can be graded properly high market value will be obtained by exporting them.
Seaweeds are able to produce a great variety of secondary metabolites that are characterized by a... more Seaweeds are able to produce a great variety of secondary metabolites that are characterized by a broad spectrum of biological activities. Two seaweeds species, namely Hypnea musciformis and Enteromorpha intestinalis were studied to evaluate the phytochemical constituency and antimicrobial activities. First of all, crude extracts of both sea weeds were prepared by two different extraction methods (soaking and water bath) using different solvents. Phytochemicals profiling results revealed the presence of bioactive compounds (flavonoids, alkaloids, tannin, saponin and phenols) in both seaweed extracts. Quantification results for ethanolic extracts of H. musciformis and E. intestinalis estimated 51 mg and 43 mg tannins in per gram of dried samples and flavonoids contents were found 67 mg and 57 mg/g mg QE/g respectively. Total phenolic contents were determined in terms of gallic acid equivalent (GAE). H. musciformis exhibited higher amount of phenolics (59 ± 0.0002 mg GAE/g) than E. in...
Pure and Applied Biology, 2017
Hypotrichosis is a heritable condition described by sparse hairs, sparse to absent eyebrows, eyel... more Hypotrichosis is a heritable condition described by sparse hairs, sparse to absent eyebrows, eyelashes, axillary, and body hair, but with normal teeth and nails. Genotyping was carried out of family from fourth generation from district Sibi, Balochistan, having two affected males and one female. Genotyping was done targeting LPAR6-linked microsatellite markers present on chromosome 13q14.11-q21.32. The exon located on LPAR6 gene, were amplified of both affected and normal individuals of the family revealing linkage at locus on chromosome 13. Sequencing result of the LPAR6, shown a recurrent missense mutation c.436G>A, p. G146R) in a family. A recurrent missense mutation revealed in the current investigation encompass the evidence of LPAR6 gene hereditary hypotrichosis.
International Journal of Contemporary Pediatrics, 2016
Background: Emergence of newer culture methods and other diagnostic aids for neonatal sepsis make... more Background: Emergence of newer culture methods and other diagnostic aids for neonatal sepsis makes evaluation of known sepsis markers imperative to define their changing role. This study was designed to assess role of CRP, leukopenia, neutropenia and thrombocytopenia in neonatal sepsis in this changing era. Methods: A prospective observational study was conducted in a level-3 NICU in north India in 2013. Study duration was 1 year. All patients visiting our hospital with non-specific symptoms and signs suggestive of sepsis were admitted and evaluated. Neonates with confirmed sepsis were allotted to cases group and others to control group. CBC and CRP were taken from both groups; sensitivity, specificity, negative and positive predictive value of leucopenia, neutropenia and thrombocytopenia were assessed. Relationship of these markers with neonatal mortality was also assessed. Statistical analysis used was Graphpad Instat statistical software was used for analysis. Comparisons were made by chi-square test and Fischer's exact test. A p-value <0.05 was taken as significant. Results: 120 neonates had confirmed sepsis and 76 were in the control group. CRP had sensitivity, specificity, negative and positive predictive values of 67%, 19%, 27% and 57%. Sensitivity, specificity, negative and positive predictive values for leukopenia were 22%, 68%, 29% and 59%, respectively. For neutropenia and thrombocytopenia these values were 16%, 81%, 34% and 61% and 41%, 71%, 42% and 70%, respectively. These markers also bore a significant association with mortality in both cases and controls (p-Value <0.05). Conclusions: Haematological parameters are poor screening tools for neonatal sepsis; however they have a definite role as prognostic indicators. CRP appears to be a good screening tool for sepsis; however its low sensitivity is a drawback. There is a need to combine CRP with other markers like procalcitonin in newer septic screens to aid early diagnosis of neonatal sepsis.
Micro & Nano Letters, 2015
This article was published in Micro and Nano Letters [© 2015 Published by The Institution of Engi... more This article was published in Micro and Nano Letters [© 2015 Published by The Institution of Engineering and Technology] and the definite version is available at: http://doi.org/10.1049/mnl.2015.0193
Annals of Clinical Microbiology and Antimicrobials, 2015
Background: For a long time mushrooms have been playing an important role in several aspects of t... more Background: For a long time mushrooms have been playing an important role in several aspects of the human activity. Recently edible mushrooms are used extensively in cooking and make part of new food in Bangladesh for their beneficial properties. The aim of this study is to screen some values of mushrooms used in Bangladesh. Methods: Methanolic extracts of 3 edible mushrooms (Pleurotus ostreatus, Lentinula edodes, Hypsizigus tessulatus) isolated from Chittagong, Bangladesh were used in this study. Phenolic compounds in the mushroom methanolic extracts were estimated by a colorimetric assay. The antioxidant activity was determined by radical 1, 1-diphenyl;-2-picrylhydrazyl (DPPH) radical scavenging assay. Eight microbial isolates were used for antimicrobial activity of methanolic extract of mushrooms by the agar well diffusion method with slight modification. Results: Determination of antimicrobial activity indicated considerable activity against all bacteria and fungi reveling zone of inhibition ranged from 7 ± 0.2 to 20 ± 0.1 mm. Minimum inhibitory concentration values of the extracts showed that they are also active even in least concentrations ranged from 1 mg/ml to 9 mg/ml. Lentinula edodes showed the best antimicrobial activity than others. Pseudomonas aeruginosa was quite resistant and Saccharomyces cerevisiae was more sensitive than others microbial isolates. Antioxidant efficiency by inhibitory concentration on 1,1-Diphenly-2-picrylhydrazyl (DPPH) was found significant when compared to standard antioxidant like ascorbic Acid. The concentration (IC50) ranged from 100 ± 1.20 to 110 ± 1.24 μg/ml. Total phenols are the major bioactive component found in extracts of isolates expressed as mg of GAE per gram of fruit body, which ranged from 3.20 ± 0.05 to 10.66 ± 0.52 mg/ml. Average concentration of flavonoid ranged from 2.50 ± 0.008 mg/ml to 4.76 ± 0.11 mg/ml; followed by very small concentration of ascorbic acid (range, 0.06 ± 0.00 mg/ml to 0.21 ± 0.01 mg/ml) in all the isolates. All the isolates showed high phenol and flavonoid content (except Pleurotus ostreatus), but ascorbic acid content was found in traces. Conclusion: This study has revealed that selected edible mushrooms have not only nutritional values but also some therapeutic values. Proper and more investigations can lead us to use these as strong medicine in future.
Wood Science and Technology, 2010
This experiment describes some anatomical features of Tectona grandis L. relating to the variatio... more This experiment describes some anatomical features of Tectona grandis L. relating to the variation of liquid permeability in radial and longitudinal directions. Safranine aqueous solution was used to measure the permeability for its brilliant color and staining properties. Even in presence of abundant rays, penetration depth of liquid in radial direction was found very low in comparison with axial flow.
International Journal of Materials Engineering Innovation
Turkish Journal of Agriculture and Forestry
This paper examines wood anatomy as it relates to safranine solution penetration in Samanea saman... more This paper examines wood anatomy as it relates to safranine solution penetration in Samanea saman, which belongs to the family Mimosoideae and is native to Bangladesh. The wood of this species was characterized by diffuse-porous vessels with simple perforations, alternate and opposite vestured intervessel pits, gums in heartwood vessels, vascicentric paratracheal axial parenchyma, lozenge-aliform, confluent prismatic crystals in fiber, and chambered axial parenchyma cells. Different anatomical features affect longitudinal and lateral liquid permeability. Herein microstructures that controlled liquid penetration are described and compared. We observed that ray cell lumen diameter, end-wall pit number and diameter, ray cell length, vessel number, and both vessel and fiber diameter and length affected the depth of liquid flow. Safranine solution formed a meniscus while traveling through different types of cells. Air trapped in capillaries blocked safranine solution penetration. Following a go-stop-go cycle, the speed of penetration slowly decreased over time.
The American Journal of Tropical Medicine and Hygiene
ABSTRACTThere are an estimated 2,000 children with β-thalassemia in the province Baluchistan of P... more ABSTRACTThere are an estimated 2,000 children with β-thalassemia in the province Baluchistan of Pakistan. These children are at high risk of acquiring transfusion-transmitted infections (TTIs) due to their need of regular blood transfusions for survival. Therefore, we investigated the frequencies of TTIs among these multi-transfused patients in a region where the WHO guidelines for blood safety are not always followed. Sera from 400 children (mean age 7.7 ± 4.70 years) treated at two thalassemia centers in Baluchistan were investigated for TTIs. Eleven (2.8%) were hepatitis B surface antigen positive, and 72 (18.3%) had anti-hepatitis C virus (HCV), two of which were infected with both viruses. Only 22% of the children had been reached by the program for universal hepatitis B virus (HBV) vaccination which started in 2004. Half (51%) of the HCV infected had also been HBV infected. The HBV- and HCV-infected patients were older and had received more blood transfusions than the uninfect...
In most epithelia, desmosomes are important adhesion structures which have a key role in linking ... more In most epithelia, desmosomes are important adhesion structures which have a key role in linking the intermediate filament network of one cell to its neighbor, thus forming a strong bond. These junctions are essential for retaining the integrity of organs, subject to mechanical stress, particularly the heart and skin. Desmogleins and desmocollins belong to super family of cadherin, which mediate adhesion at desmosomes. Through a series of protein interactions, desmosomal cadherin tails get associated with Cytoplasmic components of the desmosome, the sites of desmosome assemblage are employed with intermediate filaments. There are four types of desmogleins (Dsg1, Dsg2, Dsg3 and Dsg4) and three types of desmocollins (Dsc 1, Dsc 2, and Dsc 3). These types of desmosomal cadherins are involved in various defects. This review outlines the structure, function and associated diseases of cadherin superfamily. I believe that this review will assist researchers to enhance their basic knowledge...
It is estimated that around more than 2% of the population of world is suffering with hepatitis C... more It is estimated that around more than 2% of the population of world is suffering with hepatitis C virus and death cases because of cirrhosis and liver cancer is more than 350000.The load and epidemiology of HCV infection is different in the different parts of the world. It vary with country particular prevalence range from 10%. In comparison with USA and other urbanized countries the spread of infection in poor countries commonly outcome of contact to infected blood in society settings and healthcare centers. HCV management programs must be country specific which may be different by background and rank of economic progress. Worldwide HCV is a main cause of liver disease and in future it would be a major cause of morbidity and mortality. The complication related to the geographic allocation of HCV infection and its linked risk factor increases its progression. No vaccine is available for HCV and no later than exposure for HCV prophylaxis, so the focal point of most important escaping...
Variability in power systems is increasing due to pushing the system to limits for economic purpo... more Variability in power systems is increasing due to pushing the system to limits for economic purposes, the inclusion of new energy sources like wind turbines and photovoltaic, and the introduction of new types of loads such as electric vehicle chargers. In this new environment, system monitoring and control must keep pace to insure system stability and reliability on a wide area scale. Phasor measurement unit technology implementation is growing and can be used to provide input signals to new types of control. Fuzzy logic based power system stabilizer (PSS)
The FASEB Journal, 2016
BackgroundNon-exclusive breastfeeding and poor environmental hygiene in early infancy may perturb... more BackgroundNon-exclusive breastfeeding and poor environmental hygiene in early infancy may perturb the gut microbiota to cause local inflammation and systemic exposure to bacterial lipopolysaccharid...
Journal of emerging technologies and innovative research, 2017
PFA copy of full length research paper for possible publication in your respected journal. Kindly... more PFA copy of full length research paper for possible publication in your respected journal. Kindly acknowledge. Regards 1.Dr. Suheela chisti 2. irfan ahmed sheikh
Algorithms, 2021
Automatic Number Plate Recognition (ANPR) has been widely used in different domains, such as car ... more Automatic Number Plate Recognition (ANPR) has been widely used in different domains, such as car park management, traffic management, tolling, and intelligent transport systems. Despite this technology’s importance, the existing ANPR approaches suffer from the accurate identification of number plats due to its different size, orientation, and shapes across different regions worldwide. In this paper, we are studying these challenges by implementing a case study for smart car towing management using Machine Learning (ML) models. The developed mobile-based system uses different approaches and techniques to enhance the accuracy of recognizing number plates in real-time. First, we developed an algorithm to accurately detect the number plate’s location on the car body. Then, the bounding box of the plat is extracted and converted into a grayscale image. Second, we applied a series of filters to detect the alphanumeric characters’ contours within the grayscale image. Third, the detected th...
International Journal of Livestock Research, 2021
Journal of Advanced Biotechnology and Experimental Therapeutics, 2019
ABSTRACT: The experiments were conducted for grading unknown agarwood, to analyze the total pheno... more ABSTRACT: The experiments were conducted for grading unknown agarwood, to analyze the total phenolics, total flavonoids, antioxidant status of agarwood and its crude oil and also to identify the existing bacteria that assist in the fermentation of agarwood during soaking period. Grades of unknown agarwoods were determined based on density metric method and cross checked for ether extract. Total phenolics of agar wood and oils were analyzed through Folin-Ciocalteu method and total flavonoid contents of agar oils were estimated using Aluminium chloride colorimetric method. For the determination of antioxidant status of agarwood oil, the inhibitory effects of oil extracts on DPPH radical was measured. To identify the bacterial genus, several biochemical tests were conducted. Results showed that insect infested agarwood was classified as Grade-1 agarwood (Calculated density was 0.641 g/cm 3) and contained the highest amount ether extract of 18.90 ± 0.60%, total phenolic content of 3.5 ± 0.06 (mg GAE/g) and flavonoid content of 7.82 ± 0.23 (µg QE/ml). IC 50 values obtained by DPPH activity for Aquillaria malaccensis oil extract was found to be 0.904 µg/ml. Bacillus spp. was recognized as the mostly prevalent bacteria responsible for fermentation of agarwood which exhibit special role in increasing the yield of agar oil. If agarwood and agar oil can be graded properly high market value will be obtained by exporting them.
Seaweeds are able to produce a great variety of secondary metabolites that are characterized by a... more Seaweeds are able to produce a great variety of secondary metabolites that are characterized by a broad spectrum of biological activities. Two seaweeds species, namely Hypnea musciformis and Enteromorpha intestinalis were studied to evaluate the phytochemical constituency and antimicrobial activities. First of all, crude extracts of both sea weeds were prepared by two different extraction methods (soaking and water bath) using different solvents. Phytochemicals profiling results revealed the presence of bioactive compounds (flavonoids, alkaloids, tannin, saponin and phenols) in both seaweed extracts. Quantification results for ethanolic extracts of H. musciformis and E. intestinalis estimated 51 mg and 43 mg tannins in per gram of dried samples and flavonoids contents were found 67 mg and 57 mg/g mg QE/g respectively. Total phenolic contents were determined in terms of gallic acid equivalent (GAE). H. musciformis exhibited higher amount of phenolics (59 ± 0.0002 mg GAE/g) than E. in...
Pure and Applied Biology, 2017
Hypotrichosis is a heritable condition described by sparse hairs, sparse to absent eyebrows, eyel... more Hypotrichosis is a heritable condition described by sparse hairs, sparse to absent eyebrows, eyelashes, axillary, and body hair, but with normal teeth and nails. Genotyping was carried out of family from fourth generation from district Sibi, Balochistan, having two affected males and one female. Genotyping was done targeting LPAR6-linked microsatellite markers present on chromosome 13q14.11-q21.32. The exon located on LPAR6 gene, were amplified of both affected and normal individuals of the family revealing linkage at locus on chromosome 13. Sequencing result of the LPAR6, shown a recurrent missense mutation c.436G>A, p. G146R) in a family. A recurrent missense mutation revealed in the current investigation encompass the evidence of LPAR6 gene hereditary hypotrichosis.
International Journal of Contemporary Pediatrics, 2016
Background: Emergence of newer culture methods and other diagnostic aids for neonatal sepsis make... more Background: Emergence of newer culture methods and other diagnostic aids for neonatal sepsis makes evaluation of known sepsis markers imperative to define their changing role. This study was designed to assess role of CRP, leukopenia, neutropenia and thrombocytopenia in neonatal sepsis in this changing era. Methods: A prospective observational study was conducted in a level-3 NICU in north India in 2013. Study duration was 1 year. All patients visiting our hospital with non-specific symptoms and signs suggestive of sepsis were admitted and evaluated. Neonates with confirmed sepsis were allotted to cases group and others to control group. CBC and CRP were taken from both groups; sensitivity, specificity, negative and positive predictive value of leucopenia, neutropenia and thrombocytopenia were assessed. Relationship of these markers with neonatal mortality was also assessed. Statistical analysis used was Graphpad Instat statistical software was used for analysis. Comparisons were made by chi-square test and Fischer's exact test. A p-value <0.05 was taken as significant. Results: 120 neonates had confirmed sepsis and 76 were in the control group. CRP had sensitivity, specificity, negative and positive predictive values of 67%, 19%, 27% and 57%. Sensitivity, specificity, negative and positive predictive values for leukopenia were 22%, 68%, 29% and 59%, respectively. For neutropenia and thrombocytopenia these values were 16%, 81%, 34% and 61% and 41%, 71%, 42% and 70%, respectively. These markers also bore a significant association with mortality in both cases and controls (p-Value <0.05). Conclusions: Haematological parameters are poor screening tools for neonatal sepsis; however they have a definite role as prognostic indicators. CRP appears to be a good screening tool for sepsis; however its low sensitivity is a drawback. There is a need to combine CRP with other markers like procalcitonin in newer septic screens to aid early diagnosis of neonatal sepsis.
Micro & Nano Letters, 2015
This article was published in Micro and Nano Letters [© 2015 Published by The Institution of Engi... more This article was published in Micro and Nano Letters [© 2015 Published by The Institution of Engineering and Technology] and the definite version is available at: http://doi.org/10.1049/mnl.2015.0193
Annals of Clinical Microbiology and Antimicrobials, 2015
Background: For a long time mushrooms have been playing an important role in several aspects of t... more Background: For a long time mushrooms have been playing an important role in several aspects of the human activity. Recently edible mushrooms are used extensively in cooking and make part of new food in Bangladesh for their beneficial properties. The aim of this study is to screen some values of mushrooms used in Bangladesh. Methods: Methanolic extracts of 3 edible mushrooms (Pleurotus ostreatus, Lentinula edodes, Hypsizigus tessulatus) isolated from Chittagong, Bangladesh were used in this study. Phenolic compounds in the mushroom methanolic extracts were estimated by a colorimetric assay. The antioxidant activity was determined by radical 1, 1-diphenyl;-2-picrylhydrazyl (DPPH) radical scavenging assay. Eight microbial isolates were used for antimicrobial activity of methanolic extract of mushrooms by the agar well diffusion method with slight modification. Results: Determination of antimicrobial activity indicated considerable activity against all bacteria and fungi reveling zone of inhibition ranged from 7 ± 0.2 to 20 ± 0.1 mm. Minimum inhibitory concentration values of the extracts showed that they are also active even in least concentrations ranged from 1 mg/ml to 9 mg/ml. Lentinula edodes showed the best antimicrobial activity than others. Pseudomonas aeruginosa was quite resistant and Saccharomyces cerevisiae was more sensitive than others microbial isolates. Antioxidant efficiency by inhibitory concentration on 1,1-Diphenly-2-picrylhydrazyl (DPPH) was found significant when compared to standard antioxidant like ascorbic Acid. The concentration (IC50) ranged from 100 ± 1.20 to 110 ± 1.24 μg/ml. Total phenols are the major bioactive component found in extracts of isolates expressed as mg of GAE per gram of fruit body, which ranged from 3.20 ± 0.05 to 10.66 ± 0.52 mg/ml. Average concentration of flavonoid ranged from 2.50 ± 0.008 mg/ml to 4.76 ± 0.11 mg/ml; followed by very small concentration of ascorbic acid (range, 0.06 ± 0.00 mg/ml to 0.21 ± 0.01 mg/ml) in all the isolates. All the isolates showed high phenol and flavonoid content (except Pleurotus ostreatus), but ascorbic acid content was found in traces. Conclusion: This study has revealed that selected edible mushrooms have not only nutritional values but also some therapeutic values. Proper and more investigations can lead us to use these as strong medicine in future.
Wood Science and Technology, 2010
This experiment describes some anatomical features of Tectona grandis L. relating to the variatio... more This experiment describes some anatomical features of Tectona grandis L. relating to the variation of liquid permeability in radial and longitudinal directions. Safranine aqueous solution was used to measure the permeability for its brilliant color and staining properties. Even in presence of abundant rays, penetration depth of liquid in radial direction was found very low in comparison with axial flow.