Ercüment Aksakal | Akdeniz University (original) (raw)
Papers by Ercüment Aksakal
Anadolu tarım bilimleri dergisi, 2013
Aquaculture Reports, Apr 1, 2023
International Journal of Molecular Sciences
The aim of this study was to determine the effects of silver nanoparticles (AgNPs) on the morphol... more The aim of this study was to determine the effects of silver nanoparticles (AgNPs) on the morphology and enzymatic activity of butterfly splitfin (Ameca splendens). Individuals of both sexes, aged about five months, were exposed to AgNPs at concentrations of 0 (control group), 0.01, 0.1, and 1.0 mg/dm3 for 42 days. On the last day of the experiment, the fish were euthanized, subjected to standard histological processing (anterior intestine, liver, and gonads), and analysed for digestive enzyme activity in the anterior intestine and oxidative stress markers in the liver. Fish in the AgNP 0.01 and 0.1 groups had the lowest anterior intestinal fold and enterocyte height. However, there were no statistically significant changes in the digestive enzyme activity in the anterior intestine. Analysis of enzymatic activity in the liver showed an increase in superoxide dismutase activity in fish in the AgNP 0.1 group. Histological analyses showed that AgNPs inhibited meiotic divisions at proph...
Journal of Comparative Physiology B
Journal of Shellfish Research, 2008
A rapid polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis wa... more A rapid polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis was designed to examine genetic differentiation of some penaeid shrimps. Three hundred and fifty-six bp of cytochrome-b gene, a specific part of mitochondrial genome, was amplified with PCR to figure out differences between shrimp species Penaeus semisulcatus, Penaeus kerathurus, Parapenaeus longirostris, and Metapenaeus monoceros. PCR products were digested with different restriction endonucleases (DdeI, MboI, MboII, and MseI.). None of the tested enzymes, alone, was able to distinguish between the four shrimp species, but with the combination of the results of two digestions, all of the species could be differentiated. It was shown that PCR-RFLP method can be used to expose fraudulent substitutions of processed shrimps prior to marketing.
Journal of Animal and Feed Sciences
Journal of Enzyme Inhibition and Medicinal Chemistry
ABSTRACT Seasonal fluctuations in antioxidant (superoxide dismutase, SOD; catalase, CAT; glutathi... more ABSTRACT Seasonal fluctuations in antioxidant (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase, GPx; glutathione reductase, GR; glucose-6-phosphate dehydrogenase, G6PD) and biotransformation enzyme activities (glutathione 5-transferase, GST) and lipid peroxidation (LPO) levels of livers and gills in European catfish (Silurus glanis) and Bulatmai barbel (Barbus capito capito) were determined. Superoxide dismutase, CAT, GPx, GR, G6PD and GST activities were higher in the liver compared to gills for both species. The exception was being in B. capito capito where GR was higher in the gills compared to the than liver during autumn and winter. However, LPO levels were higher in the gills compared to the liver. At the end of present study, it was concluded that antioxidant defense system for both species were affected by many endogenous and exogenous factors. However, it is difficult to determine what is the most critical factor affecting ADS in these species because of complex effects of these factors on the antioxidant defense system.
Israeli Journal of Aquaculture (Bamidgeh), 2006
As from January 2010 The Israeli Journal of Aquaculture-Bamidgeh (IJA) will be published exclusiv... more As from January 2010 The Israeli Journal of Aquaculture-Bamidgeh (IJA) will be published exclusively as an on-line Open Access (OA) quarterly accessible by all AquacultureHub (http://www.aquaculturehub.org) members and registered individuals and institutions. Please visit our website (http://siamb.org.il) for free registration form, further information and instructions. This transformation from a subscription printed version to an on-line OA journal, aims at supporting the concept that scientific peer-reviewed publications should be made available to all, including those with limited resources. The OA IJA does not enforce author or subscription fees and will endeavor to obtain alternative sources of income to support this policy for as long as possible.
Food Chemistry, 2010
Total (TL), neutral (NL) and phospholipid (PL) amounts and fatty acid (FA) composition of female ... more Total (TL), neutral (NL) and phospholipid (PL) amounts and fatty acid (FA) composition of female Salmo trutta caspius, Salmo trutta labrax and Salmo trutta macrostigma were investigated during one year. Twenty-three FAs were identified in both NLs and PLs. The principal FAs of both fractions were palmitic acid in saturated fatty acid, oleic acid in monounsaturated fatty acid, docosahexaenoic acid
Environmental Toxicology and Pharmacology, 2013
The objective of this experiment was to test effects of different dietary lipids in rainbow trout... more The objective of this experiment was to test effects of different dietary lipids in rainbow trout feeding on the activity and expression of antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione S-transferase (GST). Four iso-nitrogenous and iso-lipidic casein-gelatin based experimental diets were formulated. The sources of dietary lipids were cod liver oil (CO, rich in polyunsaturated fatty acids), goose fat (GF, rich in saturated fatty acids and monounsaturated fatty acids), soybean oil (SO, rich in linoleic acid), and a blend of CO, GF and SO. Dietary treatments had no significant effect on growth performance and survival was not affected. SOD, GPx and GST enzymes had the maximum activity in GF diet. However qPCR data showed that SOD and GPx mRNA levels were minimum in GF group. Overall data showed that rainbow trout liver enzymes were activated upon GF diet probably activating the enzyme structure itself without stimulating gene expression.
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2011
The objective of this study was to assess the effects of cobalt and zinc exposure of rainbow trou... more The objective of this study was to assess the effects of cobalt and zinc exposure of rainbow trout (Oncorhynchus mykiss) on insulin like growth factors (IGF) and growth hormone (GH). Mature rainbow trouts were exposed to 0.42, 2.1, 4.2, 21 and 42 μmol/L Co 2+ (added as CoCl 2 •6H 2 O) and 0.34, 1.7, 3.4, 17 and 34 μmol/L Zn 2+ (added as ZnSO 4 i•7H 2 O). After 6, 12, 24 and 48 h of treatment, expressions of white muscle IGF-I, IGF-II and GH mRNAs were measured by means of quantitative Real Time PCR. During the exposure experiments, no mortalities occurred. The most effective metal concentrations, which caused significant alterations, were determined to be 42 μmol/L Co 2+ (10 mg CoCl 2 •6H 2 O/L) and 3.4 μmol/L Zn + 2 (1 mg ZnSO 4 •7H 2 O/L). The following results were obtained for these concentrations. Expression of IGF-I did not change at 6 h in zinc treatment while the decrease (p b 0.05) was observed at 12 h and 24 h, and this decrease became stronger at 48 h. Cobalt exposure caused a decrease in IGF-I mRNA level at 6 h, 12 h, 24 h and 48 h (p b 0.05). Both zinc and cobalt exposure resulted in significant decreases in GH expression at 6 h. Exposure of trout to Zn resulted in a decrease in expression of IGF-II starting from 6 h whereas the significant decrease started at 6 h in cobalt exposure and this decrease elevated at 24 h. The results indicate that micromolar cobalt and zinc exposure causes significant attenuation in the expressions of these three genes' time dependently. Our findings show that IGF-I is the most resistant and GH is the most sensitive component against cobalt and zinc exposure. We conclude that IGF/GH axis might be strongly affected by the short term exposure to low micromolar concentrations of zinc and cobalt due to alterations of these genes.
![Research paper thumbnail of Corrigendum to “The effect of peptide absorption on PepT1 gene expression and digestive system hormones in rainbow trout (Oncorhynchus mykiss)” [Comp. Biochem. Physiol. A 155 (2010) 107–114]](https://attachments.academia-assets.com/83695863/thumbnails/1.jpg)
](Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2010
Gelisen gen teknolojisine bagli olarak Restriksiyon Parcasi Uzunluk Polimorfizmi (RFLP), Rastgele... more Gelisen gen teknolojisine bagli olarak Restriksiyon Parcasi Uzunluk Polimorfizmi (RFLP), Rastgele Cogaltilmis Polimorfik DNA (RAPD), Cogaltilmis Parca Uzunluk Polimorfizmleri (AFLP), Mikrosatellitler ve Tek nukleotid Polimorfizmleri (SNP) gibi molekuler tekniklerin su urunlerinde kullanimi her gecen gun artmaktadir. Bu gen teknolojileri ile yurutulen islah calismalari dikkate degerdir. Su urunleri yetistiriciliginde islah ve yetistirme programlari icin gerekli olan bilgilerin tespiti buyuk onem arz etmektedir. Bu calismalarda molekuler tekniklerin kullanimi elde edilen bilgilerin guvenilirligini arttirmaktadir. Bu makalede mikrosatellit markirlarin su urunleri yetistiriciliginde seleksiyon amacli kullanimi ile ilgili calismalar bir araya getirilmistir
Zuhal Alım, Ercüment Aksakal, Deniz Ekinci, Orhan Erdoğan Ömer İrfan Küfrevioğlu, Şükrü Beydemir*... more Zuhal Alım, Ercüment Aksakal, Deniz Ekinci, Orhan Erdoğan Ömer İrfan Küfrevioğlu, Şükrü Beydemir* Atatürk University, Faculty of Sciences, Department of Chemistry, Biochemistry Division, Erzurum, Turkey Atatürk University, Agriculture Faculty, Department of Agricultural Biotechnology, Erzurum, Turkey Ondokuz Mayıs University, Faculty of Agriculture, Department of Agricultural Biotechnology, Samsun, Turkey Atatürk University, Biotechnology Application and Research Center, Erzurum, Turkey Atatürk University, Faculty of Sciences, Department of Molecular Biology and Genetics, Erzurum, Turkey
Anadolu tarım bilimleri dergisi, 2013
Aquaculture Reports, Apr 1, 2023
International Journal of Molecular Sciences
The aim of this study was to determine the effects of silver nanoparticles (AgNPs) on the morphol... more The aim of this study was to determine the effects of silver nanoparticles (AgNPs) on the morphology and enzymatic activity of butterfly splitfin (Ameca splendens). Individuals of both sexes, aged about five months, were exposed to AgNPs at concentrations of 0 (control group), 0.01, 0.1, and 1.0 mg/dm3 for 42 days. On the last day of the experiment, the fish were euthanized, subjected to standard histological processing (anterior intestine, liver, and gonads), and analysed for digestive enzyme activity in the anterior intestine and oxidative stress markers in the liver. Fish in the AgNP 0.01 and 0.1 groups had the lowest anterior intestinal fold and enterocyte height. However, there were no statistically significant changes in the digestive enzyme activity in the anterior intestine. Analysis of enzymatic activity in the liver showed an increase in superoxide dismutase activity in fish in the AgNP 0.1 group. Histological analyses showed that AgNPs inhibited meiotic divisions at proph...
Journal of Comparative Physiology B
Journal of Shellfish Research, 2008
A rapid polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis wa... more A rapid polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis was designed to examine genetic differentiation of some penaeid shrimps. Three hundred and fifty-six bp of cytochrome-b gene, a specific part of mitochondrial genome, was amplified with PCR to figure out differences between shrimp species Penaeus semisulcatus, Penaeus kerathurus, Parapenaeus longirostris, and Metapenaeus monoceros. PCR products were digested with different restriction endonucleases (DdeI, MboI, MboII, and MseI.). None of the tested enzymes, alone, was able to distinguish between the four shrimp species, but with the combination of the results of two digestions, all of the species could be differentiated. It was shown that PCR-RFLP method can be used to expose fraudulent substitutions of processed shrimps prior to marketing.
Journal of Animal and Feed Sciences
Journal of Enzyme Inhibition and Medicinal Chemistry
ABSTRACT Seasonal fluctuations in antioxidant (superoxide dismutase, SOD; catalase, CAT; glutathi... more ABSTRACT Seasonal fluctuations in antioxidant (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase, GPx; glutathione reductase, GR; glucose-6-phosphate dehydrogenase, G6PD) and biotransformation enzyme activities (glutathione 5-transferase, GST) and lipid peroxidation (LPO) levels of livers and gills in European catfish (Silurus glanis) and Bulatmai barbel (Barbus capito capito) were determined. Superoxide dismutase, CAT, GPx, GR, G6PD and GST activities were higher in the liver compared to gills for both species. The exception was being in B. capito capito where GR was higher in the gills compared to the than liver during autumn and winter. However, LPO levels were higher in the gills compared to the liver. At the end of present study, it was concluded that antioxidant defense system for both species were affected by many endogenous and exogenous factors. However, it is difficult to determine what is the most critical factor affecting ADS in these species because of complex effects of these factors on the antioxidant defense system.
Israeli Journal of Aquaculture (Bamidgeh), 2006
As from January 2010 The Israeli Journal of Aquaculture-Bamidgeh (IJA) will be published exclusiv... more As from January 2010 The Israeli Journal of Aquaculture-Bamidgeh (IJA) will be published exclusively as an on-line Open Access (OA) quarterly accessible by all AquacultureHub (http://www.aquaculturehub.org) members and registered individuals and institutions. Please visit our website (http://siamb.org.il) for free registration form, further information and instructions. This transformation from a subscription printed version to an on-line OA journal, aims at supporting the concept that scientific peer-reviewed publications should be made available to all, including those with limited resources. The OA IJA does not enforce author or subscription fees and will endeavor to obtain alternative sources of income to support this policy for as long as possible.
Food Chemistry, 2010
Total (TL), neutral (NL) and phospholipid (PL) amounts and fatty acid (FA) composition of female ... more Total (TL), neutral (NL) and phospholipid (PL) amounts and fatty acid (FA) composition of female Salmo trutta caspius, Salmo trutta labrax and Salmo trutta macrostigma were investigated during one year. Twenty-three FAs were identified in both NLs and PLs. The principal FAs of both fractions were palmitic acid in saturated fatty acid, oleic acid in monounsaturated fatty acid, docosahexaenoic acid
Environmental Toxicology and Pharmacology, 2013
The objective of this experiment was to test effects of different dietary lipids in rainbow trout... more The objective of this experiment was to test effects of different dietary lipids in rainbow trout feeding on the activity and expression of antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione S-transferase (GST). Four iso-nitrogenous and iso-lipidic casein-gelatin based experimental diets were formulated. The sources of dietary lipids were cod liver oil (CO, rich in polyunsaturated fatty acids), goose fat (GF, rich in saturated fatty acids and monounsaturated fatty acids), soybean oil (SO, rich in linoleic acid), and a blend of CO, GF and SO. Dietary treatments had no significant effect on growth performance and survival was not affected. SOD, GPx and GST enzymes had the maximum activity in GF diet. However qPCR data showed that SOD and GPx mRNA levels were minimum in GF group. Overall data showed that rainbow trout liver enzymes were activated upon GF diet probably activating the enzyme structure itself without stimulating gene expression.
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2011
The objective of this study was to assess the effects of cobalt and zinc exposure of rainbow trou... more The objective of this study was to assess the effects of cobalt and zinc exposure of rainbow trout (Oncorhynchus mykiss) on insulin like growth factors (IGF) and growth hormone (GH). Mature rainbow trouts were exposed to 0.42, 2.1, 4.2, 21 and 42 μmol/L Co 2+ (added as CoCl 2 •6H 2 O) and 0.34, 1.7, 3.4, 17 and 34 μmol/L Zn 2+ (added as ZnSO 4 i•7H 2 O). After 6, 12, 24 and 48 h of treatment, expressions of white muscle IGF-I, IGF-II and GH mRNAs were measured by means of quantitative Real Time PCR. During the exposure experiments, no mortalities occurred. The most effective metal concentrations, which caused significant alterations, were determined to be 42 μmol/L Co 2+ (10 mg CoCl 2 •6H 2 O/L) and 3.4 μmol/L Zn + 2 (1 mg ZnSO 4 •7H 2 O/L). The following results were obtained for these concentrations. Expression of IGF-I did not change at 6 h in zinc treatment while the decrease (p b 0.05) was observed at 12 h and 24 h, and this decrease became stronger at 48 h. Cobalt exposure caused a decrease in IGF-I mRNA level at 6 h, 12 h, 24 h and 48 h (p b 0.05). Both zinc and cobalt exposure resulted in significant decreases in GH expression at 6 h. Exposure of trout to Zn resulted in a decrease in expression of IGF-II starting from 6 h whereas the significant decrease started at 6 h in cobalt exposure and this decrease elevated at 24 h. The results indicate that micromolar cobalt and zinc exposure causes significant attenuation in the expressions of these three genes' time dependently. Our findings show that IGF-I is the most resistant and GH is the most sensitive component against cobalt and zinc exposure. We conclude that IGF/GH axis might be strongly affected by the short term exposure to low micromolar concentrations of zinc and cobalt due to alterations of these genes.
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2010
Gelisen gen teknolojisine bagli olarak Restriksiyon Parcasi Uzunluk Polimorfizmi (RFLP), Rastgele... more Gelisen gen teknolojisine bagli olarak Restriksiyon Parcasi Uzunluk Polimorfizmi (RFLP), Rastgele Cogaltilmis Polimorfik DNA (RAPD), Cogaltilmis Parca Uzunluk Polimorfizmleri (AFLP), Mikrosatellitler ve Tek nukleotid Polimorfizmleri (SNP) gibi molekuler tekniklerin su urunlerinde kullanimi her gecen gun artmaktadir. Bu gen teknolojileri ile yurutulen islah calismalari dikkate degerdir. Su urunleri yetistiriciliginde islah ve yetistirme programlari icin gerekli olan bilgilerin tespiti buyuk onem arz etmektedir. Bu calismalarda molekuler tekniklerin kullanimi elde edilen bilgilerin guvenilirligini arttirmaktadir. Bu makalede mikrosatellit markirlarin su urunleri yetistiriciliginde seleksiyon amacli kullanimi ile ilgili calismalar bir araya getirilmistir
Zuhal Alım, Ercüment Aksakal, Deniz Ekinci, Orhan Erdoğan Ömer İrfan Küfrevioğlu, Şükrü Beydemir*... more Zuhal Alım, Ercüment Aksakal, Deniz Ekinci, Orhan Erdoğan Ömer İrfan Küfrevioğlu, Şükrü Beydemir* Atatürk University, Faculty of Sciences, Department of Chemistry, Biochemistry Division, Erzurum, Turkey Atatürk University, Agriculture Faculty, Department of Agricultural Biotechnology, Erzurum, Turkey Ondokuz Mayıs University, Faculty of Agriculture, Department of Agricultural Biotechnology, Samsun, Turkey Atatürk University, Biotechnology Application and Research Center, Erzurum, Turkey Atatürk University, Faculty of Sciences, Department of Molecular Biology and Genetics, Erzurum, Turkey