Gopi Kannan | Amet University (original) (raw)
Papers by Gopi Kannan
Procedia Engineering, 2017
Wireless Communications and Mobile Computing, 2008
We propose path unaware layered routing protocol (PULRP) for 2D underwater sensor networks (UWSNs... more We propose path unaware layered routing protocol (PULRP) for 2D underwater sensor networks (UWSNs) with mobile nodes. The steady-state distribution of mobile nodes in UWSNs is nonuniform in general. Hence, we use a mobility model-dependent node distribution. The proposed PULRP algorithm consists of two phases. In the first phase (layering phase), a layering structure is presented which is a set of concentric circles, around a sink node. The radii of the concentric circles are chosen based on equal distribution of nodes in every layer. A distributed power control mechanism is also introduced. The power level of nodes in a particular layer is chosen such that communication occurs only with nodes in the next layer. In PULRP, we consider multihop communication from source to sink. Therefore, in the second phase (communication phase), we propose a method to choose the intermediate relay nodes and an on the fly routing algorithm for packet delivery from source node to sink node across the chosen relay nodes. The proposed algorithm, PULRP finds the routing path on the fly and hence, it does not require any fixed routing table, localization, or time synchronization processes. We demonstrate the performance of PULRP using random waypoint (RWP) mobility model in a simulated underwater environment. Our findings show that the proposed algorithm has considerably better throughput (successful packet delivery rate) compared to the underwater diffusion (UWD) algorithm for various node densities as well as node velocities. In addition, the delay performance of PULRP is also better than that of UWD.
In the Present study, Docking analysis was performed with Nitrocefin and it's analogs to determin... more In the Present study, Docking analysis was performed with Nitrocefin and it's analogs to determine their binding efficiency at the binding pocket of Pencillin binding protein 2a of S.aureus. As a result, based on the docking scores generated by the Software Glide, Two of the Nitrocefin analogs have scores nearer to that of nitrocefin. This indicates that this class of analogous compounds can be generated as new set of anti bacterial drugs against S. aureus.
Nowadays, the pharmaceutical market is growing rapidly and continuously in worldwide but, still t... more Nowadays, the pharmaceutical market is growing rapidly and continuously in worldwide but, still the demand for new drug discovery is encouraged. Because, the growth of numbers drug resistant infectious disease and more upcoming disorders to human and animals. In general, the marine animals especially mollusks and their compounds constitute a practically unlimited resource of new active substances. Hence, the present study was carried out to determine the bactericidal activity of Crassostrea madrasensis protein against human pathogens. The edible Oyster Crassostrea madrasensis was collected from Rayapuram lnading centre, Tamil Nadu, India. Immediately it was extracted by using phosphate buffer at three different pH (4, 7 and 9) and all the extracts were screened against six different human pathogens such as Vibrio cholerae, V. parahaemolyticus, Salmonella sp, Shigella sp, Streptococcus sp and Staphylococcus sp by agar well diffusion assay. After 24 hrs of incubation the maximum inhibitory effect was observed against Vibrio parahaemolyticus, Streptococcus sp and Staphylococcus sp and the minimum inhibitory effect was observed against Vibrio cholerae, Salmonella sp and Shigella sp respectively. Whereas checking the minimal inhibitory concentration (MIC), the crude protein extract of Crassostrea madrasensis was inhibited the bacterial strains with the minimum inhibitory concentration of not less than 0.1ml (100¼l). The molecular weight of the crude protein was found from 12.2 to 74.2 kDa and the total protein content of phosphate buffer crude extract of Crassostrea madrasensis was found to be 312 ¼g/ mg. From, the results, the work has suggested to use this commercially available and protein rich (bactericidal) oyster in therapeutics for the development of novel antibiotics against multiple drug resistance (MDR) pathogenic microbes.
The present study was carried out to degrade the textile Reactive azo dyes by using bacteria isol... more The present study was carried out to degrade the textile Reactive azo dyes by using bacteria isolated from dye contaminated soil. The effect of pH, temperature, carbon, nitrogen souces, inoculum size and incubation time was studied with an aim to determine the optimal conditions required for maximum decolorization. The bacterial strains used in the study were Bacillus licheniformis and Pseudomonas putida. The optimum pH for decolorization of RR 195 by P. putida was 7.0. It shows good decolorization efficiency even in alkaline region. The optimum temperature was 37 0 C. Glucose and Yeast extract has emerged as the ideal carbon and nitrogen sources for both bacteria. In different inoculum size and incubation time evaluated, 3.0% v/v and 120 hrs appeared to highest percentage of decolorization process by both bacterial strains.
The dyes discharged from different industries are the source of organic pollutants which are intr... more The dyes discharged from different industries are the source of organic pollutants which are introduced into the natural environment and spoil the biotic as well as the abiotic systems. Although, decolourization of these dyes from water is a challenging process to the industries. Recently, the use of fungi or its enzymes for dye decolourization is an alternative method. In this present study, three different Coastal sand dune plants such as, Canavalia rosea, Ipomea pescaprae and Spinifex sp. were collected from Kanathur Coastal area, East Coast of Tamil Nadu, India. Morphologically 29 different endophytic fungal strains were isolated from different segments (Leaf, Stem, and Root) of sand dunes plants, and they were named as AEF01 to AEF29. These strains were qualitatively screened for laccase production by agar well diffusion method. Among that, four strains namely, AEF17, AEF19, AEF22 and AEF25 have showed maximum laccase production, and they were cultured for laccase production in solid state and submerged fermentation. Aafter the incubation period the laccase was qualitatively assayed. The endophytic fungal strain AEF17 produced maximum laccase in solid state fermentation and it was potentially chosen for optimization studies in one parameter at a time method under different parameters such as pH, temperature, salinity, incubation period and different cheaper substrates. The laccase production was found to be superior at when using a wheat bran as substrate and the optimum pH, temperature, salinity and incubation time were found to be 5, 40 0 C,5% and 168 hours. So, the potential fungal strain AEF17 was mass cultured in optimized medium and the laccase was partially purified by ammonium sulphate precipitation and dialysis. The partially purified laccase was immobilized in sodium alginate beads and tested for their ability to decolorize nine different textile dyes. From the results the maximum decolourization activity was found in Blue M2R (BM2R), Black-B (BB) and Orange M2R(OM2R) followed by minimum decolourization activity in Yellow MR(YMR), Red BSID (RBSID), Manenta MP (MMP), Blue MR (BMR), Orange 3R (O3R) and Brown GR (BGR) decolourization. Based on the microscopic identification, the endophytic fungal strain AEF17 was belonging to Fusarium sp.
Procedia Engineering, 2017
Wireless Communications and Mobile Computing, 2008
We propose path unaware layered routing protocol (PULRP) for 2D underwater sensor networks (UWSNs... more We propose path unaware layered routing protocol (PULRP) for 2D underwater sensor networks (UWSNs) with mobile nodes. The steady-state distribution of mobile nodes in UWSNs is nonuniform in general. Hence, we use a mobility model-dependent node distribution. The proposed PULRP algorithm consists of two phases. In the first phase (layering phase), a layering structure is presented which is a set of concentric circles, around a sink node. The radii of the concentric circles are chosen based on equal distribution of nodes in every layer. A distributed power control mechanism is also introduced. The power level of nodes in a particular layer is chosen such that communication occurs only with nodes in the next layer. In PULRP, we consider multihop communication from source to sink. Therefore, in the second phase (communication phase), we propose a method to choose the intermediate relay nodes and an on the fly routing algorithm for packet delivery from source node to sink node across the chosen relay nodes. The proposed algorithm, PULRP finds the routing path on the fly and hence, it does not require any fixed routing table, localization, or time synchronization processes. We demonstrate the performance of PULRP using random waypoint (RWP) mobility model in a simulated underwater environment. Our findings show that the proposed algorithm has considerably better throughput (successful packet delivery rate) compared to the underwater diffusion (UWD) algorithm for various node densities as well as node velocities. In addition, the delay performance of PULRP is also better than that of UWD.
In the Present study, Docking analysis was performed with Nitrocefin and it's analogs to determin... more In the Present study, Docking analysis was performed with Nitrocefin and it's analogs to determine their binding efficiency at the binding pocket of Pencillin binding protein 2a of S.aureus. As a result, based on the docking scores generated by the Software Glide, Two of the Nitrocefin analogs have scores nearer to that of nitrocefin. This indicates that this class of analogous compounds can be generated as new set of anti bacterial drugs against S. aureus.
Nowadays, the pharmaceutical market is growing rapidly and continuously in worldwide but, still t... more Nowadays, the pharmaceutical market is growing rapidly and continuously in worldwide but, still the demand for new drug discovery is encouraged. Because, the growth of numbers drug resistant infectious disease and more upcoming disorders to human and animals. In general, the marine animals especially mollusks and their compounds constitute a practically unlimited resource of new active substances. Hence, the present study was carried out to determine the bactericidal activity of Crassostrea madrasensis protein against human pathogens. The edible Oyster Crassostrea madrasensis was collected from Rayapuram lnading centre, Tamil Nadu, India. Immediately it was extracted by using phosphate buffer at three different pH (4, 7 and 9) and all the extracts were screened against six different human pathogens such as Vibrio cholerae, V. parahaemolyticus, Salmonella sp, Shigella sp, Streptococcus sp and Staphylococcus sp by agar well diffusion assay. After 24 hrs of incubation the maximum inhibitory effect was observed against Vibrio parahaemolyticus, Streptococcus sp and Staphylococcus sp and the minimum inhibitory effect was observed against Vibrio cholerae, Salmonella sp and Shigella sp respectively. Whereas checking the minimal inhibitory concentration (MIC), the crude protein extract of Crassostrea madrasensis was inhibited the bacterial strains with the minimum inhibitory concentration of not less than 0.1ml (100¼l). The molecular weight of the crude protein was found from 12.2 to 74.2 kDa and the total protein content of phosphate buffer crude extract of Crassostrea madrasensis was found to be 312 ¼g/ mg. From, the results, the work has suggested to use this commercially available and protein rich (bactericidal) oyster in therapeutics for the development of novel antibiotics against multiple drug resistance (MDR) pathogenic microbes.
The present study was carried out to degrade the textile Reactive azo dyes by using bacteria isol... more The present study was carried out to degrade the textile Reactive azo dyes by using bacteria isolated from dye contaminated soil. The effect of pH, temperature, carbon, nitrogen souces, inoculum size and incubation time was studied with an aim to determine the optimal conditions required for maximum decolorization. The bacterial strains used in the study were Bacillus licheniformis and Pseudomonas putida. The optimum pH for decolorization of RR 195 by P. putida was 7.0. It shows good decolorization efficiency even in alkaline region. The optimum temperature was 37 0 C. Glucose and Yeast extract has emerged as the ideal carbon and nitrogen sources for both bacteria. In different inoculum size and incubation time evaluated, 3.0% v/v and 120 hrs appeared to highest percentage of decolorization process by both bacterial strains.
The dyes discharged from different industries are the source of organic pollutants which are intr... more The dyes discharged from different industries are the source of organic pollutants which are introduced into the natural environment and spoil the biotic as well as the abiotic systems. Although, decolourization of these dyes from water is a challenging process to the industries. Recently, the use of fungi or its enzymes for dye decolourization is an alternative method. In this present study, three different Coastal sand dune plants such as, Canavalia rosea, Ipomea pescaprae and Spinifex sp. were collected from Kanathur Coastal area, East Coast of Tamil Nadu, India. Morphologically 29 different endophytic fungal strains were isolated from different segments (Leaf, Stem, and Root) of sand dunes plants, and they were named as AEF01 to AEF29. These strains were qualitatively screened for laccase production by agar well diffusion method. Among that, four strains namely, AEF17, AEF19, AEF22 and AEF25 have showed maximum laccase production, and they were cultured for laccase production in solid state and submerged fermentation. Aafter the incubation period the laccase was qualitatively assayed. The endophytic fungal strain AEF17 produced maximum laccase in solid state fermentation and it was potentially chosen for optimization studies in one parameter at a time method under different parameters such as pH, temperature, salinity, incubation period and different cheaper substrates. The laccase production was found to be superior at when using a wheat bran as substrate and the optimum pH, temperature, salinity and incubation time were found to be 5, 40 0 C,5% and 168 hours. So, the potential fungal strain AEF17 was mass cultured in optimized medium and the laccase was partially purified by ammonium sulphate precipitation and dialysis. The partially purified laccase was immobilized in sodium alginate beads and tested for their ability to decolorize nine different textile dyes. From the results the maximum decolourization activity was found in Blue M2R (BM2R), Black-B (BB) and Orange M2R(OM2R) followed by minimum decolourization activity in Yellow MR(YMR), Red BSID (RBSID), Manenta MP (MMP), Blue MR (BMR), Orange 3R (O3R) and Brown GR (BGR) decolourization. Based on the microscopic identification, the endophytic fungal strain AEF17 was belonging to Fusarium sp.