İlham Eröz Poyraz | Anadolu University (original) (raw)

Papers by İlham Eröz Poyraz

Journal of Faculty Pharmacy of Istanbul University, 2007

Journal of Applied Pharmaceutical Science, 2015

Biological Diversity and Conservation, 2019

Turkish Journal of Biology

Turkish Journal of Botany

In this study, pollen and seed morphology of Velezia L. (Caryophyllaceae) species in Turkey were ... more In this study, pollen and seed morphology of Velezia L. (Caryophyllaceae) species in Turkey were investigated by light and scanning electron microscopy. The genus is represented by 5 species in Turkey: V. tunicoides P.H.Davis, V. hispida Boiss., V. quadridentata Sibth. & Sm., V. pseudorigida Hub.-Mor. and V. rigida L. Endemism ratio of the genus is 60%, which shows that Turkey is the gene center for this genus. The pollen grains of Velezia species are radially symmetrical and isopolar, oblate-spheroidal, operculate, and polypantoporate, pores with conical spinules on operculum. Pollen ornamentation, pore numbers, exine thickness, pore diameter, operculum ornamentation, and spinule sizes are varying characters between Velezia species. Seeds of Velezia species are bright black when mature; the embryo is straight, the cylindrical seeds are dorsiventrally compressed usually with incurved and thickened margins; the hilum being situated in the centre of the concave surface. Number of seeds in capsule, seed length and width, presence of tubercle, suture shape and the number of suture points per plate are important characters in differentiating Velezia species.

Turkish Journal of Biology, 2012

Random amplifi ed polymorphic DNA (RAPD) markers were used to determine genetic relationships amo... more Random amplifi ed polymorphic DNA (RAPD) markers were used to determine genetic relationships among Velezia L. species from Turkey. A total of 432 amplifi ed bands were obtained using 14 RAPD primers. Th e polymorphism in RAPD markers was high (98.61%) and was suffi cient to distinguish each species. Th e degree of band-sharing was used for evaluating the genetic similarity between species and for constructing a dendrogram by the unweighted pair group method with arithmetic mean (UPGMA). Genetic relationships among the species were found to be fully consistent with those obtained by the use of morphological characters. Data obtained from our study demonstrated that the RAPD technique could be successfully used for the determination of genetic relationships among Velezia species and for species identifi cation. Furthermore, it can be effi ciently employed in future studies to provide preliminary data for conservation of endangered Velezia species.

Journal of the International Society of Antioxidants in Nutrition & Health, 2016

Lycium L. species has been used as both food and medicine for thousand years in China, although t... more Lycium L. species has been used as both food and medicine for thousand years in China, although they are not known well in Turkey, In this study, the methanol (ME), acetone (ACE) and boiling water (BW) extracts from fruits of Lycium ruthenicum, L. shawii var. leptophyllum and L. depressum (Solanaceae), grown in Turkey, were screened for their phytochemical, antiproliferative and antioxidant properties. Total phenolic contents (TPC) of the extracts were measured by the Folin–Ciocalteau assay and phenolic acids were also determined by RP-HPLC-DAD[1]. b-carotene bleaching and free radical scavenging assays were used to determine total antioxidant activity of fruit extracts and the results were compared to those of BHT. In addition, the cytotoxicity of the extracts on NIH/3T3 mouse fibroblast cells were measured by cell proliferation reagent WST-1 for 24 and 48h [2]. TPC of the extracts from L. species varied from 21.26±0.75 to 326.24±0.97 GAE/g extract. Chlorogenic, caffeic and v...

Along with present study it is designed to examine phenolic compositions, in vitro antioxidant an... more Along with present study it is designed to examine phenolic compositions, in vitro antioxidant and cytotoxicity activities of methanol and ethyl acetate extracts of Salvia aethiopis L. and S. ceratophylla L. (Lamiaceae) from Turkey. Total phenolic contents of S. aethiopis methanol (SA-ME) and ethyl acetate (SA-EA) extracts were between 94.36±1.36-290.62±1.51 mg GAE/g extract while S. ceratophylla methanol (SC-ME) and ethyl acetate (SC-EA) extracts were between 168.35±1.97-330.14±2.28 mg GAE/g extract. The main phenolic acid of the methanol and ethyl acetate extracts was rosmarinic acid (40.25 and 140.6 μg/100 g plant for S. aethiopis; 74 and 234.5 μg/100 g plant for S. ceratophylla). Phenolic acids of S. aethiopis and S. ceratophylla extracts has much more cinnamic acid derivatives then benzoic acid derivatives. Studied Salvia extracts showed dose-dependent radical scavenging activities. HPLC results allow to make a correlation between antioxidant capacity and quantity of these phen...

Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 2021

Asetilkolinesteraz (AChE) ve bütirilkolinesteraz (BChE) inhibisyonu, beyinde β-amiloit birikimini... more Asetilkolinesteraz (AChE) ve bütirilkolinesteraz (BChE) inhibisyonu, beyinde β-amiloit birikimini azaltmak ve asetilkolin kullanımını artırmada, yani Alzheimer hastalığının (AD) tedavisinde önemli hedeflerden biridir. Oksidatif stres nedeniyle oluşan serbest radikallerin beyinde nöron ve metal akümülasyonuna verdiği hasar AD'nin patojeneziyle doğrudan ilişkilidir. Amaryllidaceae familyası üyelerinin (Galanthus, Leucojum v.b.) içerdiği galantamin, senile demansın tedavisinde kullanılan AChE inhibitörlerinden biridir. Bu çalışmada, bu familyanın üyesi nergis (Narcissus L.) kültür çeşitlerinin soğanlarına ait metanol:kloroform (1:1 (v:v)) ekstrelerinin, toplam fenolik madde ve polifenol içerikleri belirlenmiştir. Ayrıca ekstrelerin in vitro antioksidan aktiviteleri, AChE ve BChEe inhibisyonları ile Alzheimer tedavisi arasındaki ilişkinin araştırılması amaçlanmıştır. Ekstrelerin toplam fenolik madde miktarları 20.78 ± 0.25 ile 57.97 ± 0.49 mg gallik asit eşdeğeri (GAE)/g ekstre olarak tespit edilmiş, toplam fenolik madde miktarı bakımından en zengin kültür çeşidinin 'Golden Ducat' olduğu görülmüştür. Yüksek Basınçlı Sıvı Kromatografisi-Diyod Dizisi Dedektörü (DDD) ile çeşitlere göre belirlenen fenolik asitler, 'Golden Ducat', gallik asit ve protokateşik asit; 'Golden Harvest', para-hidroksibenzoik asit; 'Carlton', klorojenik asit; 'Cheerfulnes', para-kumarik asit; 'Einstein', ferulik asit şeklindedir. DPPH radikal süpürücü etki açısından 'Einstein' kültür çeşidinin inhibisyon değeri 23.65 ± 0.73 (9.6 × 10-4 derişimde), 'Cheerfulnes' kültür çeşidinin inhibisyon değeri 26.69 ± 1.04 (1.8 × 10-3 derişimde) ve 28.51 ± 0.1 (3.6 × 10-3 derişimde) olarak bulunmuştur. 'Carlton' kültür çeşidinden elde edilen ekstre 0.2 mg/mL derişimde %14.61 ile en yüksek metal şelatlayıcı antioksidan etki göstermiştir (Etilendiamin Tetraasetik Asit (EDTA): 77.84). 'Strong Gold' çeşidi ekstresi 1.19 (mg/mL) %50 Etkili Derişim (EC50) değeri ile indirgeyici güç ölçümünde diğer türlere göre daha yüksek etki göstermiştir (Bütil Hidroksi Toluen (BHT)'nin %50 Etkili Derişimi (EC50): 0.21), askorbik asit EC50: 0.094). AChE'nin inhibitor etkisi, 'Tête-à-Tête' kültür çeşidi, 200 µg/mL derişimde %53.36 (aynı derişimde donepezil % 92.077) olarak bulunmuştur. BChE inhibitör etki tayininde ise yine 'Tête-à-Tête' kültür çeşidi 200 µg/mL derişimde %30.25 ve 100 µg/mL derişimde %26.8 inhibisyon göstermiştir.

Indian Journal of Pharmaceutical Education and Research, 2018

Backround: The RAPD-and ISSR-PCR techniques are offering an insight into the detecting the genoto... more Backround: The RAPD-and ISSR-PCR techniques are offering an insight into the detecting the genotoxicity of Gentiana extracts. Objective: It is aimed with the present study, to detect the genotoxicity of methanol extracts of ten Turkish Gentiana L. taxa on germinated Allium cepa L. root tips. Methods: RAPD-and ISSR-PCR techniques were used for detection of genotoxicity of Gentiana extracts. Results: Four RAPD and three ISSR primers produced the reproducible polymorphic and monomorphic banding patterns among 10 RAPD and 10 ISSR primers for all DNA samples. It is not any serious alteration along with band intensity change, the disappearance of the bands, and appearance of the new bands in the band profiles amplified from the Gentiana extracts-treated genomic DNA sample of A. cepa. The most efficient results were obtained by RAPD-P9 and ISSR-1 primer among the seven productive primers. Discussion: It is not observed any variation in the RAPD-and ISSR-PCR band profiles in time and concentration-dependent manner. Conclusion: It is determined that the three different concentrations of Gentiana extracts did not interact with the A. cepa DNA.

Records of Natural Products, 2018

Purple nutsedge, Cyperus rotundus L. (Cyperaceae) is an invasive plant grown in all around the wo... more Purple nutsedge, Cyperus rotundus L. (Cyperaceae) is an invasive plant grown in all around the world. C. rotundus rhizomes are called "topalak" and an analgesic plant for the treatment of stomach ache in infants, folk medicine in Turkey. The volatile compounds of C. rotundus rhizomes were investigated by Headspace-SPME. Cyperene (30.5% and 28.0%), α-copaene (10.6% and12%) and α-ylangene (7.7% and10.5%) were identified as main volatile components of rhizomes and analyzed at room temperature and 40ºC respectively. This study designed to support detailed studies on underground plant parts volatiles and it is the first time to identification of volatile compounds of raw C. rotundus rhizomes collected from Turkey by Headspace-SPME and GC and GC-MS.

Free Radical Biology and Medicine, 2016

Seaweeds are potentially excellent sources of bioactive metabolites that could represent useful l... more Seaweeds are potentially excellent sources of bioactive metabolites that could represent useful leads in the development of new functional ingredients in pharmaceutical and cosmetic industries. In the last decade, new marine bioprocess technologies have allowed the isolation of substances with biological properties. The brown alga Sargassum muticum (Yendo) Fensholt (Ochrophyta) was enzymatically hydrolyzed to prepare water-soluble extracts by using six different commercially available carbohydratedegrading enzymes and two proteases. Evaluation of 2,2diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) antioxidant, tyrosinase, elastase, and biofilm inhibition, antibacterial and antiviral activities as well as evaluation of cytotoxicity were realized for each extract. Total phenolic content was measured for extract characterization, and solid-phase extraction was useful to purify the enzymatic extract. Soluble total phenolic content of S. muticum Viscozyme extract was highest with 6.4% of dry weight. Enzymatic Celluclast and Viscozyme extracts had the lowest value of DPPH IC 50 indicating a strong antiradical activity, 0.6 mg mL −1 , in comparison with other enzymes. The ferric reducing antioxidant power ranged between 48.7 μM Fe 2+ Eq, digested with Viscozyme, and 60.8 μM Fe 2+ Eq, digested with Amyloglucosidase. Tyrosinase inhibition activity of S. muticum Neutrase extract was 41.3% higher compared to other enzymes. Elastase inhibition activity of S. muticum Shearzyme extract had highest activity (32.8%). All enzymatic extracts showed no cytotoxic effect towards the kidney Vero cells. Meanwhile, only S. muticum Neutrase and Alcalase extracts exhibited potential antiviral activity. In addition, S. muticum Viscozyme and Shearzyme extracts showed promising activity in suppressing the biofilm formation against Pseudomonas aeruginosa and Escherichia coli, respectively. Purification of S. muticum Viscozyme extracts by solid-phase extraction managed to concentrate the phenolic content and improve the bioactivity. These results indicate the promising potential of enzyme-assisted followed by solid-phase extraction in recovering phenolic content and in improving its bioactivity.

ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology, 2016

Volatile compounds of green and roasted beans of Coffea arabica L. (Rubiaceae) were investigated ... more Volatile compounds of green and roasted beans of Coffea arabica L. (Rubiaceae) were investigated by this study. Volatile compounds were trapped with Headspace Solid-Phase Microextraction (HS-SPME) technique and analyzed by Gas Chromatography-Mass Spectrometry (GC/MS). The main components of the green coffee were identified as isoamylalcohol (10.4%), hexanal (10.4%) and hexacosane (8.2%) while furfurylalcohol (13.6%), furfurylacetate (10.7%) and 5-methyl furfural (9.27%) were identified as the main components of the roasted coffee. In conclusion, using HP-SPME-GC-MS, it was possible to quantify different volatile compouns like as alcohols, aldehydes, ketones, pyrazines, pyridines, and furans in green and roasted coffee beans which belong to different chemical classes.

Biological Diversity and Conservation, 2021

In this study, Origanum majorana L., Origanum vulgare L. subsp. vulgare, Salvia tomentosa Mill. a... more In this study, Origanum majorana L., Origanum vulgare L. subsp. vulgare, Salvia tomentosa Mill. and Sideritis athoa Papan. & Kokkini are sold in local bazaars in Edremit-Akçay (Balıkesir, Turkey) and used as folk medicine. They were investigated for their local names and essential oils. Aerial parts of the plants were subjected to hydrodistillation using a Clevenger type apparatus. Essential oils were analyzed using gas chromatography (GC) and gas chromatography mass spectrometry (GC / MS). The main constituents of Origanum majorana were carvacrol (71.8%), -terpinen (8.9%) and p-simen (8.2%). The major component was identified as carvacrol (76.1%) along with γterpinene (6.7%) and p-simen (6.4%) in the oil of Origanum vulgare subsp. vulgare. Camphor (27.3%), -pinene (15.3%), 1,8-cineol (12.3%) and -pinene (7.8%) were found as the major compounds in the oil of Salvia tomentosa. Carvacrol (33.0%), epicubebole (10.0%) and -pinene (7.3%) as the major components in the oil of Sideritis athoa. Due to the main essential oil compounds, carvacrol, cafur, etc. of these plants have potential use in orthodontics, endodontics, and periodontology in dentistry.

Phytotaxa, 2016

A study of the genetic relationships among Petrorhagia taxa from Turkey was carried out using int... more A study of the genetic relationships among Petrorhagia taxa from Turkey was carried out using inter-simple sequence repeat (ISSR) markers. A total of 409 amplified bands were obtained by 10 ISSR primers. The polymorphism ratio was high (100%) across 45 individuals representing nine Petrorhagia taxa (P. dubia, P. prolifera, P. pamphylica, P. peroninii, P. saxifraga, P. cretica, P. alpina subsp. alpina, P. alpina subsp. olympica, P. lycica) and was sufficient to distinguish each species. Statistical analyses were performed by using POPGENE, GenAlEx6, and PAUP. An unweighted pair-group method with arithmetic mean (UPGMA) dendrogram was constructed based on Nei’s genetic distance along with outgroup species (Velezia rigida) in MEGA4. The dendrogram shows two main clusters, the first one (Cluster-I) included only P. lycica, while the cluster-II contained all other taxa. Cluster-II can be grouped in two sub-clusters, with P. prolifera and P. saxifraga constituting a first sub-cluster, the...

ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology, 2016

Wild and cultivated populations of Momordica charantia L. (Cucurbitaceae) are pantropical distrib... more Wild and cultivated populations of Momordica charantia L. (Cucurbitaceae) are pantropical distribution in the world. The plant is known as "kudret nare" and "sarı kadak" in Kosovo and "kudret narı" in Turkey. Mature fruits of the species contain resin and momordicine and use as stomachic, emolient, wound healing and purgative. Detailed morpho-anatomical properties of M. charantia collected from Kosovo/Prizren were investigated in the present study.

Journal of Faculty Pharmacy of Istanbul University, 2007

Journal of Applied Pharmaceutical Science, 2015

Biological Diversity and Conservation, 2019

Turkish Journal of Biology

Turkish Journal of Botany

In this study, pollen and seed morphology of Velezia L. (Caryophyllaceae) species in Turkey were ... more In this study, pollen and seed morphology of Velezia L. (Caryophyllaceae) species in Turkey were investigated by light and scanning electron microscopy. The genus is represented by 5 species in Turkey: V. tunicoides P.H.Davis, V. hispida Boiss., V. quadridentata Sibth. & Sm., V. pseudorigida Hub.-Mor. and V. rigida L. Endemism ratio of the genus is 60%, which shows that Turkey is the gene center for this genus. The pollen grains of Velezia species are radially symmetrical and isopolar, oblate-spheroidal, operculate, and polypantoporate, pores with conical spinules on operculum. Pollen ornamentation, pore numbers, exine thickness, pore diameter, operculum ornamentation, and spinule sizes are varying characters between Velezia species. Seeds of Velezia species are bright black when mature; the embryo is straight, the cylindrical seeds are dorsiventrally compressed usually with incurved and thickened margins; the hilum being situated in the centre of the concave surface. Number of seeds in capsule, seed length and width, presence of tubercle, suture shape and the number of suture points per plate are important characters in differentiating Velezia species.

Turkish Journal of Biology, 2012

Random amplifi ed polymorphic DNA (RAPD) markers were used to determine genetic relationships amo... more Random amplifi ed polymorphic DNA (RAPD) markers were used to determine genetic relationships among Velezia L. species from Turkey. A total of 432 amplifi ed bands were obtained using 14 RAPD primers. Th e polymorphism in RAPD markers was high (98.61%) and was suffi cient to distinguish each species. Th e degree of band-sharing was used for evaluating the genetic similarity between species and for constructing a dendrogram by the unweighted pair group method with arithmetic mean (UPGMA). Genetic relationships among the species were found to be fully consistent with those obtained by the use of morphological characters. Data obtained from our study demonstrated that the RAPD technique could be successfully used for the determination of genetic relationships among Velezia species and for species identifi cation. Furthermore, it can be effi ciently employed in future studies to provide preliminary data for conservation of endangered Velezia species.

Journal of the International Society of Antioxidants in Nutrition & Health, 2016

Lycium L. species has been used as both food and medicine for thousand years in China, although t... more Lycium L. species has been used as both food and medicine for thousand years in China, although they are not known well in Turkey, In this study, the methanol (ME), acetone (ACE) and boiling water (BW) extracts from fruits of Lycium ruthenicum, L. shawii var. leptophyllum and L. depressum (Solanaceae), grown in Turkey, were screened for their phytochemical, antiproliferative and antioxidant properties. Total phenolic contents (TPC) of the extracts were measured by the Folin–Ciocalteau assay and phenolic acids were also determined by RP-HPLC-DAD[1]. b-carotene bleaching and free radical scavenging assays were used to determine total antioxidant activity of fruit extracts and the results were compared to those of BHT. In addition, the cytotoxicity of the extracts on NIH/3T3 mouse fibroblast cells were measured by cell proliferation reagent WST-1 for 24 and 48h [2]. TPC of the extracts from L. species varied from 21.26±0.75 to 326.24±0.97 GAE/g extract. Chlorogenic, caffeic and v...

Along with present study it is designed to examine phenolic compositions, in vitro antioxidant an... more Along with present study it is designed to examine phenolic compositions, in vitro antioxidant and cytotoxicity activities of methanol and ethyl acetate extracts of Salvia aethiopis L. and S. ceratophylla L. (Lamiaceae) from Turkey. Total phenolic contents of S. aethiopis methanol (SA-ME) and ethyl acetate (SA-EA) extracts were between 94.36±1.36-290.62±1.51 mg GAE/g extract while S. ceratophylla methanol (SC-ME) and ethyl acetate (SC-EA) extracts were between 168.35±1.97-330.14±2.28 mg GAE/g extract. The main phenolic acid of the methanol and ethyl acetate extracts was rosmarinic acid (40.25 and 140.6 μg/100 g plant for S. aethiopis; 74 and 234.5 μg/100 g plant for S. ceratophylla). Phenolic acids of S. aethiopis and S. ceratophylla extracts has much more cinnamic acid derivatives then benzoic acid derivatives. Studied Salvia extracts showed dose-dependent radical scavenging activities. HPLC results allow to make a correlation between antioxidant capacity and quantity of these phen...

Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 2021

Asetilkolinesteraz (AChE) ve bütirilkolinesteraz (BChE) inhibisyonu, beyinde β-amiloit birikimini... more Asetilkolinesteraz (AChE) ve bütirilkolinesteraz (BChE) inhibisyonu, beyinde β-amiloit birikimini azaltmak ve asetilkolin kullanımını artırmada, yani Alzheimer hastalığının (AD) tedavisinde önemli hedeflerden biridir. Oksidatif stres nedeniyle oluşan serbest radikallerin beyinde nöron ve metal akümülasyonuna verdiği hasar AD'nin patojeneziyle doğrudan ilişkilidir. Amaryllidaceae familyası üyelerinin (Galanthus, Leucojum v.b.) içerdiği galantamin, senile demansın tedavisinde kullanılan AChE inhibitörlerinden biridir. Bu çalışmada, bu familyanın üyesi nergis (Narcissus L.) kültür çeşitlerinin soğanlarına ait metanol:kloroform (1:1 (v:v)) ekstrelerinin, toplam fenolik madde ve polifenol içerikleri belirlenmiştir. Ayrıca ekstrelerin in vitro antioksidan aktiviteleri, AChE ve BChEe inhibisyonları ile Alzheimer tedavisi arasındaki ilişkinin araştırılması amaçlanmıştır. Ekstrelerin toplam fenolik madde miktarları 20.78 ± 0.25 ile 57.97 ± 0.49 mg gallik asit eşdeğeri (GAE)/g ekstre olarak tespit edilmiş, toplam fenolik madde miktarı bakımından en zengin kültür çeşidinin 'Golden Ducat' olduğu görülmüştür. Yüksek Basınçlı Sıvı Kromatografisi-Diyod Dizisi Dedektörü (DDD) ile çeşitlere göre belirlenen fenolik asitler, 'Golden Ducat', gallik asit ve protokateşik asit; 'Golden Harvest', para-hidroksibenzoik asit; 'Carlton', klorojenik asit; 'Cheerfulnes', para-kumarik asit; 'Einstein', ferulik asit şeklindedir. DPPH radikal süpürücü etki açısından 'Einstein' kültür çeşidinin inhibisyon değeri 23.65 ± 0.73 (9.6 × 10-4 derişimde), 'Cheerfulnes' kültür çeşidinin inhibisyon değeri 26.69 ± 1.04 (1.8 × 10-3 derişimde) ve 28.51 ± 0.1 (3.6 × 10-3 derişimde) olarak bulunmuştur. 'Carlton' kültür çeşidinden elde edilen ekstre 0.2 mg/mL derişimde %14.61 ile en yüksek metal şelatlayıcı antioksidan etki göstermiştir (Etilendiamin Tetraasetik Asit (EDTA): 77.84). 'Strong Gold' çeşidi ekstresi 1.19 (mg/mL) %50 Etkili Derişim (EC50) değeri ile indirgeyici güç ölçümünde diğer türlere göre daha yüksek etki göstermiştir (Bütil Hidroksi Toluen (BHT)'nin %50 Etkili Derişimi (EC50): 0.21), askorbik asit EC50: 0.094). AChE'nin inhibitor etkisi, 'Tête-à-Tête' kültür çeşidi, 200 µg/mL derişimde %53.36 (aynı derişimde donepezil % 92.077) olarak bulunmuştur. BChE inhibitör etki tayininde ise yine 'Tête-à-Tête' kültür çeşidi 200 µg/mL derişimde %30.25 ve 100 µg/mL derişimde %26.8 inhibisyon göstermiştir.

Indian Journal of Pharmaceutical Education and Research, 2018

Backround: The RAPD-and ISSR-PCR techniques are offering an insight into the detecting the genoto... more Backround: The RAPD-and ISSR-PCR techniques are offering an insight into the detecting the genotoxicity of Gentiana extracts. Objective: It is aimed with the present study, to detect the genotoxicity of methanol extracts of ten Turkish Gentiana L. taxa on germinated Allium cepa L. root tips. Methods: RAPD-and ISSR-PCR techniques were used for detection of genotoxicity of Gentiana extracts. Results: Four RAPD and three ISSR primers produced the reproducible polymorphic and monomorphic banding patterns among 10 RAPD and 10 ISSR primers for all DNA samples. It is not any serious alteration along with band intensity change, the disappearance of the bands, and appearance of the new bands in the band profiles amplified from the Gentiana extracts-treated genomic DNA sample of A. cepa. The most efficient results were obtained by RAPD-P9 and ISSR-1 primer among the seven productive primers. Discussion: It is not observed any variation in the RAPD-and ISSR-PCR band profiles in time and concentration-dependent manner. Conclusion: It is determined that the three different concentrations of Gentiana extracts did not interact with the A. cepa DNA.

Records of Natural Products, 2018

Purple nutsedge, Cyperus rotundus L. (Cyperaceae) is an invasive plant grown in all around the wo... more Purple nutsedge, Cyperus rotundus L. (Cyperaceae) is an invasive plant grown in all around the world. C. rotundus rhizomes are called "topalak" and an analgesic plant for the treatment of stomach ache in infants, folk medicine in Turkey. The volatile compounds of C. rotundus rhizomes were investigated by Headspace-SPME. Cyperene (30.5% and 28.0%), α-copaene (10.6% and12%) and α-ylangene (7.7% and10.5%) were identified as main volatile components of rhizomes and analyzed at room temperature and 40ºC respectively. This study designed to support detailed studies on underground plant parts volatiles and it is the first time to identification of volatile compounds of raw C. rotundus rhizomes collected from Turkey by Headspace-SPME and GC and GC-MS.

Free Radical Biology and Medicine, 2016

Seaweeds are potentially excellent sources of bioactive metabolites that could represent useful l... more Seaweeds are potentially excellent sources of bioactive metabolites that could represent useful leads in the development of new functional ingredients in pharmaceutical and cosmetic industries. In the last decade, new marine bioprocess technologies have allowed the isolation of substances with biological properties. The brown alga Sargassum muticum (Yendo) Fensholt (Ochrophyta) was enzymatically hydrolyzed to prepare water-soluble extracts by using six different commercially available carbohydratedegrading enzymes and two proteases. Evaluation of 2,2diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) antioxidant, tyrosinase, elastase, and biofilm inhibition, antibacterial and antiviral activities as well as evaluation of cytotoxicity were realized for each extract. Total phenolic content was measured for extract characterization, and solid-phase extraction was useful to purify the enzymatic extract. Soluble total phenolic content of S. muticum Viscozyme extract was highest with 6.4% of dry weight. Enzymatic Celluclast and Viscozyme extracts had the lowest value of DPPH IC 50 indicating a strong antiradical activity, 0.6 mg mL −1 , in comparison with other enzymes. The ferric reducing antioxidant power ranged between 48.7 μM Fe 2+ Eq, digested with Viscozyme, and 60.8 μM Fe 2+ Eq, digested with Amyloglucosidase. Tyrosinase inhibition activity of S. muticum Neutrase extract was 41.3% higher compared to other enzymes. Elastase inhibition activity of S. muticum Shearzyme extract had highest activity (32.8%). All enzymatic extracts showed no cytotoxic effect towards the kidney Vero cells. Meanwhile, only S. muticum Neutrase and Alcalase extracts exhibited potential antiviral activity. In addition, S. muticum Viscozyme and Shearzyme extracts showed promising activity in suppressing the biofilm formation against Pseudomonas aeruginosa and Escherichia coli, respectively. Purification of S. muticum Viscozyme extracts by solid-phase extraction managed to concentrate the phenolic content and improve the bioactivity. These results indicate the promising potential of enzyme-assisted followed by solid-phase extraction in recovering phenolic content and in improving its bioactivity.

ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology, 2016

Volatile compounds of green and roasted beans of Coffea arabica L. (Rubiaceae) were investigated ... more Volatile compounds of green and roasted beans of Coffea arabica L. (Rubiaceae) were investigated by this study. Volatile compounds were trapped with Headspace Solid-Phase Microextraction (HS-SPME) technique and analyzed by Gas Chromatography-Mass Spectrometry (GC/MS). The main components of the green coffee were identified as isoamylalcohol (10.4%), hexanal (10.4%) and hexacosane (8.2%) while furfurylalcohol (13.6%), furfurylacetate (10.7%) and 5-methyl furfural (9.27%) were identified as the main components of the roasted coffee. In conclusion, using HP-SPME-GC-MS, it was possible to quantify different volatile compouns like as alcohols, aldehydes, ketones, pyrazines, pyridines, and furans in green and roasted coffee beans which belong to different chemical classes.

Biological Diversity and Conservation, 2021

In this study, Origanum majorana L., Origanum vulgare L. subsp. vulgare, Salvia tomentosa Mill. a... more In this study, Origanum majorana L., Origanum vulgare L. subsp. vulgare, Salvia tomentosa Mill. and Sideritis athoa Papan. & Kokkini are sold in local bazaars in Edremit-Akçay (Balıkesir, Turkey) and used as folk medicine. They were investigated for their local names and essential oils. Aerial parts of the plants were subjected to hydrodistillation using a Clevenger type apparatus. Essential oils were analyzed using gas chromatography (GC) and gas chromatography mass spectrometry (GC / MS). The main constituents of Origanum majorana were carvacrol (71.8%), -terpinen (8.9%) and p-simen (8.2%). The major component was identified as carvacrol (76.1%) along with γterpinene (6.7%) and p-simen (6.4%) in the oil of Origanum vulgare subsp. vulgare. Camphor (27.3%), -pinene (15.3%), 1,8-cineol (12.3%) and -pinene (7.8%) were found as the major compounds in the oil of Salvia tomentosa. Carvacrol (33.0%), epicubebole (10.0%) and -pinene (7.3%) as the major components in the oil of Sideritis athoa. Due to the main essential oil compounds, carvacrol, cafur, etc. of these plants have potential use in orthodontics, endodontics, and periodontology in dentistry.

Phytotaxa, 2016

A study of the genetic relationships among Petrorhagia taxa from Turkey was carried out using int... more A study of the genetic relationships among Petrorhagia taxa from Turkey was carried out using inter-simple sequence repeat (ISSR) markers. A total of 409 amplified bands were obtained by 10 ISSR primers. The polymorphism ratio was high (100%) across 45 individuals representing nine Petrorhagia taxa (P. dubia, P. prolifera, P. pamphylica, P. peroninii, P. saxifraga, P. cretica, P. alpina subsp. alpina, P. alpina subsp. olympica, P. lycica) and was sufficient to distinguish each species. Statistical analyses were performed by using POPGENE, GenAlEx6, and PAUP. An unweighted pair-group method with arithmetic mean (UPGMA) dendrogram was constructed based on Nei’s genetic distance along with outgroup species (Velezia rigida) in MEGA4. The dendrogram shows two main clusters, the first one (Cluster-I) included only P. lycica, while the cluster-II contained all other taxa. Cluster-II can be grouped in two sub-clusters, with P. prolifera and P. saxifraga constituting a first sub-cluster, the...

ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology, 2016

Wild and cultivated populations of Momordica charantia L. (Cucurbitaceae) are pantropical distrib... more Wild and cultivated populations of Momordica charantia L. (Cucurbitaceae) are pantropical distribution in the world. The plant is known as "kudret nare" and "sarı kadak" in Kosovo and "kudret narı" in Turkey. Mature fruits of the species contain resin and momordicine and use as stomachic, emolient, wound healing and purgative. Detailed morpho-anatomical properties of M. charantia collected from Kosovo/Prizren were investigated in the present study.