The Clinical Proteomic Technologies for Cancer (original) (raw)

Aldo-Keto Reductase Family 1 Member B1

Background

Catalog Number:

CPTC-AKR1B1-1

RRID:

AB_2273742

Target Antigen:

Aldo-Keto Reductase Family 1 Member B1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

02/25/2025

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
Characterization Data [Compare Characterization Data]

CyTOF

Click to enlarge image Imaging mass cytometry on lung cancer tissue core using CPTC-AKR1B1-1 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (appendix and kidney) and cancer tissues (breast, lung, and ovarian). Click image to enlarge

Result: Positive

Imaging mass cytometry on lung cancer tissue core using CPTC-AKR1B1-1 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (appendix and kidney) and cancer tissues (breast, lung, and ovarian).

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression (red). Click image to enlarge

Result: Positive

Protein Array in which CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression (red).

Western Blot - Recombinant Protein or Peptide

Click to enlarge image Western Blot using CPTC-AKR1B1-1 as primary Ab against Ag 10316 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

Result: Positive

Western Blot using CPTC-AKR1B1-1 as primary Ab against Ag 10316 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Western Blot - Tissue or Cell Lysate

Click to enlarge image Western Blot using CPTC-AKR1B1-1 as primary Ab against cell lysate from TK-10 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

Result: Positive

Western Blot using CPTC-AKR1B1-1 as primary Ab against cell lysate from TK-10 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Background

Catalog Number:

CPTC-AKR1B1-2

RRID:

AB_1553409

Target Antigen:

Aldo-Keto Reductase Family 1 Member B1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

02/25/2025

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
Characterization Data [Compare Characterization Data]

Immunofluorescence - HPA

Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Human assay: A-431 fixed with PFA, dilution: 1:2000 Click image to enlarge

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Human assay: A-431 fixed with PFA, dilution: 1:2000

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

Result: High Binding

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-AKR1B1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

Result: Positive

Protein Array in which CPTC-AKR1B1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Western Blot - Recombinant Protein or Peptide

Click to enlarge image Western Blot using CPTC-AKR1B1-2 as primary Ab against Ag 10316 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

Result: Positive

Western Blot using CPTC-AKR1B1-2 as primary Ab against Ag 10316 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Western Blot - Tissue or Cell Lysate

Click to enlarge image Western Blot using CPTC-AKR1B1-2 as primary Ab against cell lysate from TK-10 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

Result: Positive

Western Blot using CPTC-AKR1B1-2 as primary Ab against cell lysate from TK-10 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Background

NCI Identification Number:

10316

Antigen Name:

Aldo-Keto Reductase Family 1 Member B1

CPTC Name:

CPTC-AKR1B1

Aliases:

AKR1B1; ADR; ALDR1; ALR2; AR; EC 1.1.1.21; MGC1804

Function:

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic
complications by catalyzing the reduction of glucose to sorbitol. There are a few putative
pseudogenes for this gene, and one of them has been confirmed and mapped to chromosome 3.

It functions to catalyze the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.

Chromosomal Localization:

7q35

Expression System:

E. Coli

Accession Number:

BC000260

UniProt Accession Number:

P15121

DNA Source:

HIP:HsCD00003028

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

49578

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMASRLLLNNGAKMPILGLGTWKSPPGQVTEAVKVAIDVGYRHIDCAH
VYQNENEVGVAIQEKLREQVVKREELFIVSKLWCTYHEKGLVKGACQKTL
SDLKLDYLDLYLIHWPTGFKPGKEFFPLDESGNVVPSDTNILDTWAAMEE
LVDEGLVKAIGISNFNHLQVEMILNKPGLKYKPAVNQIECHPYLTQEKLI
QYCQSKGIVVTAYSPLGSPDRPWAKPEDPSLLEDPRIKAIAAKHNKTTAQ
VLIRFPMQRNLVVIPKSVTPERIAENFKVFDFELSSQDMTTLLSYNRNWR
VCALLSCTSHKDYPFHEEF

Native Sequence:

MASRLLLNNGAKMPILGLGTWKSPPGQVTEAVKVAIDVGYRHIDCAHVYQ
NENEVGVAIQEKLREQVVKREELFIVSKLWCTYHEKGLVKGACQKTLSDL
KLDYLDLYLIHWPTGFKPGKEFFPLDESGNVVPSDTNILDTWAAMEELVD
EGLVKAIGISNFNHLQVEMILNKPGLKYKPAVNQIECHPYLTQEKLIQYC
QSKGIVVTAYSPLGSPDRPWAKPEDPSLLEDPRIKAIAAKHNKTTAQVLI
RFPMQRNLVVIPKSVTPERIAENFKVFDFELSSQDMTTLLSYNRNWRVCA
LLSCTSHKDYPFHEEF

Calculated Isoelectric Point:

6.51

Molecular Weight:

36126

Last Updated:

08/22/2020

Characterization Data

Gel

Click to enlarge image PAGE of Ag 10316 with molecular weight standards
Click image to enlarge

Ag 10316

PAGE of Ag 10316 with molecular weight standards

SOPs

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