The Clinical Proteomic Technologies for Cancer (original) (raw)

Aldo-keto Reductase Family 1 Member C1

Background

Catalog Number:

CPTC-AKR1C1-1

RRID:

AB_2617218

Target Antigen:

Aldo-keto Reductase Family 1 Member C1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

10/24/2019

Antigen Recognition(s):

Recombinant Full-length

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Characterization Data [Compare Characterization Data]

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Western Blot - Recombinant Protein or Peptide

Click to enlarge image Western Blot using CPTC-AKR1C1-1 as primary Ab against rAg 10013 (AKR1C1) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3). Click image to enlarge

Result: Positive

Western Blot using CPTC-AKR1C1-1 as primary Ab against rAg 10013 (AKR1C1) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).

Background

Catalog Number:

CPTC-AKR1C1-2

Target Antigen:

Aldo-keto Reductase Family 1 Member C1

Isotype:

IgG3

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length

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Characterization Data [Compare Characterization Data]

Immunofluorescence - HPA

Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & endoplasmic reticulum. Human assay: Hep G2 fixed with PFA, dilution: 1:175. Click image to enlarge

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & endoplasmic reticulum. Human assay: Hep G2 fixed with PFA, dilution: 1:175.

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Western Blot - Recombinant Protein or Peptide

Click to enlarge image Western Blot using CPTC-AKR1C1-2 as primary Ab against rAg 10013 (AKR1C1) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3). Click image to enlarge

Result: Positive

Western Blot using CPTC-AKR1C1-2 as primary Ab against rAg 10013 (AKR1C1) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).

Background

NCI Identification Number:

10013

Antigen Name:

Aldo-keto Reductase Family 1 Member C1

CPTC Name:

CPTC-AKR1C1

Aliases:

AKR1C1; 2-ALPHA-HSD; 20-ALPHA-HSD; C9; DD1; DD1/DD2; DDH; DDH1; H-37; EC 1.1.1.-,EC 1.1.1.149,EC 1.3.1.20; HAKRC; HBAB; MBAB; MGC8954; OTTHUMP00000018992

Function:

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Converts progesterone to its inactive form, 20-alpha-dihydroxyprogesterone(20-alpha-OHP). In the liver and intestine, may have a role in the transport of bile. May have a role in monitoring the intrahepatic bile acid concentration. Has a low bile-binding ability. May
play a role in myelin formation.

Chromosomal Localization:

10p15-p14

Expression System:

E. Coli

Accession Number:

NM_001353

UniProt Accession Number:

Q04828

DNA Source:

INV : 4662654

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

37610

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMDSKYQCVKLNDGHFMPVLGFGTYAPAEVPKSKALEATKLAIEAGFR
HIDSAHLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWCNSHRPELVRP
ALERSLKNLQLDYVDLYLIHFPVSVKPGEEVIPKDENGKILFDTVDLCAT
WEAVEKCKDAGLAKSIGVSNFNRRQLEMILNKPGLKYKPVCNQVECHPYF
NQRKLLDFCKSKDIVLVAYSALGSHREEPWVDPNSPVLLEDPVLCALAKK
HKRTPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTSEEMKAID
GLNRNVRYLTLDIFAGPPNYPFSDEY

Native Sequence:

MDSKYQCVKLNDGHFMPVLGFGTYAPAEVPKSKALEATKLAIEAGFRHID
SAHLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWCNSHRPELVRPALE
RSLKNLQLDYVDLYLIHFPVSVKPGEEVIPKDENGKILFDTVDLCATWEA
VEKCKDAGLAKSIGVSNFNRRQLEMILNKPGLKYKPVCNQVECHPYFNQR
KLLDFCKSKDIVLVAYSALGSHREEPWVDPNSPVLLEDPVLCALAKKHKR
TPALIALRYQLQRGVVVLAKSYNEQRIRQNVQVFEFQLTSEEMKAIDGLN
RNVRYLTLDIFAGPPNYPFSDEY

Calculated Isoelectric Point:

8.01

Molecular Weight:

37061

Last Updated:

09/01/2008

Characterization Data

Gel

Click to enlarge image PAGE of Ag 10013 (with molecular weight standards in lane 1)
Click image to enlarge

Ag 10013

PAGE of Ag 10013 (with molecular weight standards in lane 1)

SOPs

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