The Clinical Proteomic Technologies for Cancer (original) (raw)

APEX Nuclease I

Background

Catalog Number:

CPTC-APEX1-2

RRID:

AB_10804789

Target Antigen:

APEX Nuclease I

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

05/23/2024

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
Characterization Data [Compare Characterization Data]

CyTOF

Click to enlarge image Imaging mass cytometry on lung cancer tissue core using CPTC-APEX1-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, placenta, prostate, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidney) and cancer tissues (breast, lung prostate, colon, and ovarian). Click image to enlarge

Result: Positive

Imaging mass cytometry on lung cancer tissue core using CPTC-APEX1-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, placenta, prostate, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidney) and cancer tissues (breast, lung prostate, colon, and ovarian).

Immunofluorescence - HPA

Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm. Human assay: A-431 fixed with PFA, dilution: 1:2000 Human assay: U-2 OS fixed with PFA, dilution: 1:2000 Human assay: U-251 MG fixed with PFA, dilution: 1:2000 Click image to enlarge

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm. Human assay: A-431 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000
Human assay: U-251 MG fixed with PFA, dilution: 1:2000

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-APEX1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

Result: Positive

Protein Array in which CPTC-APEX1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Western Blot - Recombinant Protein or Peptide

Click to enlarge image Western Blot using CPTC-APEX1-2 as primary Ab against Ag 10750 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

Result: Positive

Western Blot using CPTC-APEX1-2 as primary Ab against Ag 10750 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Background

NCI Identification Number:

10750

Antigen Name:

APEX Nuclease I

CPTC Name:

CPTC-APEX1

Aliases:

APE; APE-1; APE1; APEN; APEX, APX; HAP1; EC 4.2.99.18; REF1; REF-1; OTTHUMP00000163988

Function:

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.

Repairs oxidative DNA damages in vitro. May have a role in protection against cell lethality and suppression of mutations. Removes the blocking groups from the 3'-termini of the DNA strand breaks generated by ionizing radiations and bleomycin

Chromosomal Localization:

14q11.2

Expression System:

E. Coli

Accession Number:

BC004979.1

UniProt Accession Number:

P27695

DNA Source:

xxx

Immunogen:

Recombinant Full Length Protein

Vector Name:

HIP : HsCD00003335

Extinction Coefficient:

56568

Buffers:

PBS

Expressed Sequence:

SNAMPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDKEAAGEGPALYED
PPDQKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQET
KCSENKLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIG
DEEHDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLK
GLASRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAV
PLADSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCD
SKIRSKALGSDHCPITLYLAL

Native Sequence:

MPKRGKKGAVAEDGDELRTEPEAKKSKTAAKKNDKEAAGEGPALYEDPPD
QKTSPSGKPATLKICSWNVDGLRAWIKKKGLDWVKEEAPDILCLQETKCS
ENKLPAELQELPGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYGIGDEE
HDQEGRVIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWDEAFRKFLKGLA
SRKPLVLCGDLNVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQAVPLA
DSFRHLYPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSHSLLPALCDSKI
RSKALGSDHCPITLYLAL

Calculated Isoelectric Point:

8.33

Molecular Weight:

35.55

Last Updated:

06/17/2020

Characterization Data

SOPs

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