The Clinical Proteomic Technologies for Cancer (original) (raw)

CD27 Molecule Peptide 1

Background

Catalog Number:

CPTC-CD27-1

Target Antigen:

CD27 Molecule Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

10/31/2023

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]
Background

Catalog Number:

CPTC-CD27-2

Target Antigen:

CD27 Molecule Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

10/31/2023

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]
Background

Catalog Number:

CPTC-CD27-3

Target Antigen:

CD27 Molecule Peptide 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length

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Characterization Data [Compare Characterization Data]

Western Blot - Tissue or Cell Lysate

Click to enlarge image Western blot using CPTC-CD27-3 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 29.1 kDa. Molecular weight standards are also included (lane 1). MCF7 and NCI-H226 cell lines are positive. All other cell lines are negative. Click image to enlarge

Result: Positive

Western blot using CPTC-CD27-3 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 29.1 kDa. Molecular weight standards are also included (lane 1). MCF7 and NCI-H226 cell lines are positive. All other cell lines are negative.

Western Blot - Tissue or Cell Lysate

Click to enlarge image Western blot using CPTC-CD27-3 as primary antibody against human lung (2), spleen (3), endometrium (4) , breast (5), and ovary (6) tissue lysates. The expected molecular weight is 29.1 kDa. Vinculin was used as a loading control. Click image to enlarge

Result: Presumed Positive (with additional bands)

Western blot using CPTC-CD27-3 as primary antibody against human lung (2), spleen (3), endometrium (4) , breast (5), and ovary (6) tissue lysates. The expected molecular weight is 29.1 kDa. Vinculin was used as a loading control.

Background

NCI Identification Number:

00467

Antigen Name:

CD27 Molecule Peptide 1

CPTC Name:

CPTC-CD27 Peptide 1

Aliases:

CD27 Molecule; Tumor Necrosis Factor Receptor Superfamily, Member 7; T-Cell Activation Antigen CD27; CD27L Receptor; CD27 Antigen; TNFRSF7; S152; Tp55; T14; Tumor Necrosis Factor Receptor Superfamily Member 7; T Cell Activation Antigen S152; S152. LPFS2; CD27

Function:

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.CD27 (CD27 Molecule) is a Protein Coding gene. Diseases associated with CD27 include Lymphoproliferative Syndrome 2 and Combined T Cell And B Cell Immunodeficiency. Among its related pathways are TNF Superfamily - Human Ligand-Receptor Interactions and their Associated Functions and Akt Signaling. Gene Ontology (GO) annotations related to this gene include transmembrane signaling receptor activity and cysteine-type endopeptidase inhibitor activity involved in apoptotic process. An important paralog of this gene is TNFRSF9.Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Chromosomal Localization:

12p13.31

Accession Number:

NP_001233.2

UniProt Accession Number:

P26842

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

EEEGSTIPIQEDYR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1540

Last Updated:

12/16/2020

Characterization Data

SOPs

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