The Clinical Proteomic Technologies for Cancer (original) (raw)
CD70 Molecule Peptide 1
Background
Catalog Number:
CPTC-CD70-1
Target Antigen:
CD70 Molecule Peptide 1
Isotype:
IgG2b
Species:
Mouse Recombinant Cloned Antibody
Last Updated:
05/23/2024
Antigen Recognition(s):
Peptide, Endogenous
Thematic Panel(s):
Immuno-Oncology (IO)
External Links
- CPTAC Assay Portal CPTAC-5959
Publications
Characterization Data [Compare Characterization Data]
Result: Positive
Protein Array in which CPTC-CD70-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Western Blot - Tissue or Cell Lysate
Result: Positive
Western blot using CPTC-CD70-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 21.1 kDa and 23.4 kDa. Molecular weight standards are also included (lane 1). A549, HeLa and MCF7 are positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.
Western Blot - Tissue or Cell Lysate
Result: Positive
Automated western blot using CPTC-CD70-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 21.1 kDa and 23.4 kDa. Molecular weight standards are also included (lane 1). A549 is positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.
Background
Catalog Number:
CPTC-CD70-2
Target Antigen:
CD70 Molecule Peptide 1
Isotype:
IgG
Species:
Mouse Monoclonal Antibody
Last Updated:
02/02/2024
Antigen Recognition(s):
Peptide
Purchase
- DSHB CPTC-CD70-2
Characterization Data [Compare Characterization Data]
Background
Catalog Number:
CPTC-CD70-3
Target Antigen:
CD70 Molecule Peptide 1
Isotype:
IgG
Species:
Mouse Monoclonal Antibody
Last Updated:
02/02/2024
Antigen Recognition(s):
Peptide
Purchase
- DSHB CPTC-CD70-3
Characterization Data [Compare Characterization Data]
Background
NCI Identification Number:
00470
Antigen Name:
CD70 Molecule Peptide 1
CPTC Name:
CPTC-CD70 Peptide 1
Aliases:
CD70 Molecule; CD27L; Tumor Necrosis Factor Ligand Superfamily Member 7; CD70 Antigen; CD27 Ligand; CD27-L; CD27LG; TNFSF7; Tumor Necrosis Factor (Ligand) Superfamily, Member 7; Tumor Necrosis Factor Ligand 8A; Surface Antigen CD70; Ki-24 Antigen; TNLG8A; LPFS3; CD70
Function:
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis. CD70 (CD70 Molecule) is a Protein Coding gene. Diseases associated with CD70 include Lymphoproliferative Syndrome 3 and Acute Myocarditis. Among its related pathways are PEDF Induced Signaling and TNF Superfamily - Human Ligand-Receptor Interactions and their Associated Functions. Gene Ontology (GO) annotations related to this gene include signaling receptor binding and protease binding.Cytokine which is the ligand for CD27. The CD70-CD27 pathway plays an important role in the generation and maintenance of T cell immunity, in particular during antiviral responses. Upon CD27 binding, induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Chromosomal Localization:
19p13.3
Accession Number:
NP_001243.1
UniProt Accession Number:
P32970
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
LYWQGGPALGR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1210
Last Updated:
12/16/2020
Links
- EnsEMBL - EnsEMBL CD70
- Entrez - Entrez CD70
- Uniprot - Uniprot P32970
Characterization Data
SOPs
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