The Clinical Proteomic Technologies for Cancer (original) (raw)

Creatine Kinase B Chain

Background

NCI Identification Number:

10328

Antigen Name:

Creatine Kinase B Chain

CPTC Name:

CPTC-CKB

Aliases:

CKB; B-CK; CKBB; EC 2.7.3.2

Function:

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The
encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a
heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.

Chromosomal Localization:

14q32

Expression System:

E. Coli

Accession Number:

BC019281

UniProt Accession Number:

P12277

DNA Source:

HIP:HsCD00004710

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

35535

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMPFSNSHNALKLRFPAEDEFPDLSAHNNHMAKVLTPELYAELRAKST
PSGFTLDDVIQTGVDNPGHPYIMTVGCVAGDEESYEVFKDLFDPIIEDRH
GGYKPSDEHKTDLNPDNLQGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCS
RGERRAIEKLAVEALSSLDGDLAGRYYALKSMTEAEQQQLIDDHFLFDKP
VSPLLLASGMARDWPDARGIWHNDNKTFLVWVNEEDHLRVISMQKGGNMK
EVFTRFCTGLTQIETLFKSKDYEFMWNPHLGYILTCPSNLGTGLRAGVHI
KLPNLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNADRLGFSEV
ELVQMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK

Native Sequence:

MPFSNSHNALKLRFPAEDEFPDLSAHNNHMAKVLTPELYAELRAKSTPSG
FTLDDVIQTGVDNPGHPYIMTVGCVAGDEESYEVFKDLFDPIIEDRHGGY
KPSDEHKTDLNPDNLQGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGE
RRAIEKLAVEALSSLDGDLAGRYYALKSMTEAEQQQLIDDHFLFDKPVSP
LLLASGMARDWPDARGIWHNDNKTFLVWVNEEDHLRVISMQKGGNMKEVF
TRFCTGLTQIETLFKSKDYEFMWNPHLGYILTCPSNLGTGLRAGVHIKLP
NLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNADRLGFSEVELV
QMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK

Calculated Isoelectric Point:

5.34

Molecular Weight:

42917

Last Updated:

08/22/2020

Links

• EnsEMBL - EnsEMBL CKB
• Entrez - Entrez CKB
• Uniprot - Uniprot P12277

Characterization Data

Gel

Click image to enlarge

Ag 10328

PAGE of Ag 10328 with molecular weight standards (lane 1)

SOPs

• BL21 Competant Cells (25.5 KB)

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