The Clinical Proteomic Technologies for Cancer (original) (raw)

Colony Stimulating Factor 2 Receptor Subunit Alpha Peptide 1

Background

Catalog Number:

CPTC-CSF2RA-1

Target Antigen:

Colony Stimulating Factor 2 Receptor Subunit Alpha Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length

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Characterization Data [Compare Characterization Data]

Western Blot - Recombinant Protein or Peptide

Click to enlarge image Western blot using CPTC-CSF2RA-1 as primary antibody against human colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) (CSF2RA), transcript variant 1, recombinant protein (lane 2). Expected molecular weight - 44.1 kDa. Molecular weight standards are also included (lane 1). Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein. Click image to enlarge

Result: Positive

Western blot using CPTC-CSF2RA-1 as primary antibody against human colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) (CSF2RA), transcript variant 1, recombinant protein (lane 2). Expected molecular weight - 44.1 kDa. Molecular weight standards are also included (lane 1). Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.

Western Blot - Tissue or Cell Lysate

Click to enlarge image Automated western blot using CPTC-CSF2RA-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 46.2 kDa, 46.9 kDa, 38.4 kDa, 32.9 kDa, 43.5 kDa, 26.6 kDa, 49.4 kDa, 31.1 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

Result: Negative

Automated western blot using CPTC-CSF2RA-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 46.2 kDa, 46.9 kDa, 38.4 kDa, 32.9 kDa, 43.5 kDa, 26.6 kDa, 49.4 kDa, 31.1 kDa. Molecular weight standards are also included (lane 1).

Western Blot - Tissue or Cell Lysate

Click to enlarge image Western blot using CPTC-CSF2RA-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 46.2 kDa, 46.9 kDa, 38.4 kDa, 32.9 kDa, 43.5 kDa, 26.6 kDa, 49.9 kDa, and 31.1 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

Result: Negative

Western blot using CPTC-CSF2RA-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 46.2 kDa, 46.9 kDa, 38.4 kDa, 32.9 kDa, 43.5 kDa, 26.6 kDa, 49.9 kDa, and 31.1 kDa. Molecular weight standards are also included (lane 1).

Background

NCI Identification Number:

00429

Antigen Name:

Colony Stimulating Factor 2 Receptor Subunit Alpha Peptide 1

CPTC Name:

CPTC-CSF2RA Peptide 1

Aliases:

Colony Stimulating Factor 2 Receptor Subunit Alpha; Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage); Granulocyte-Macrophage Colony-Stimulating Factor Receptor Subunit Alpha; Alpha-GM-CSF Receptor; GM-CSF-R-Alpha; CD116 Antigen; GMCSFR-Alpha; GMR-Alpha; AlphaGMR; CDw116; CSF2RY; CD116; CSF2R; Granulocyte-Macrophage Colony-Stimulating Factor Receptor Alpha Chain; Colony Stimulating Factor 2 Receptor Alpha Subunit; GM-CSF Receptor Alpha Subunit; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; CSF2RA; SMDP4; GMR

Function:

The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble.CSF2RA (Colony Stimulating Factor 2 Receptor Subunit Alpha) is a Protein Coding gene. Diseases associated with CSF2RA include Surfactant Metabolism Dysfunction, Pulmonary, 4 and Hereditary Pulmonary Alveolar Proteinosis. Among its related pathways are RET signaling and Signaling by GPCR. Gene Ontology (GO) annotations related to this gene include cytokine receptor activity. An important paralog of this gene is IL3RA.Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.

Chromosomal Localization:

Accession Number:

NP_001155001.1

UniProt Accession Number:

P15509

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

NTQPGTENLLINVSGDLENR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

2200

Last Updated:

09/21/2020

Characterization Data

SOPs

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