The Clinical Proteomic Technologies for Cancer (original) (raw)
Cystatin B
Background
Catalog Number:
CPTC-CSTB-1
RRID:
AB_2617239
Target Antigen:
Cystatin B
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
05/23/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-CSTB-1
Characterization Data [Compare Characterization Data]
Result: Positive
Imaging mass cytometry on lung cancer tissue core using CPTC-CSTB-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (lung, testis, and endometrium) and cancer tissue (lung).
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Background
Catalog Number:
CPTC-CSTB-2
RRID:
AB_2617240
Target Antigen:
Cystatin B
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
05/23/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-CSTB-2
Characterization Data [Compare Characterization Data]
Result: Positive
Imaging mass cytometry on lung cancer tissue core using CPTC-CSTB-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (breast, lung, testis, endometrium, and appendix) and cancer tissues (lung, breast, ovarian, and colon).
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Background
Catalog Number:
CPTC-CSTB-3
RRID:
AB_2617241
Target Antigen:
Cystatin B
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
05/23/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-CSTB-3
External Links
- Human Protein Atlas CPTC-CSTB-3
Characterization Data [Compare Characterization Data]
Result: Positive
Imaging mass cytometry on normal lung cancer tissue core using CPTC-CSTB-3 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, breast, lung, testis, endometrium, and appendix) and cancer tissues (lung, breast, and ovarian).
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Western Blot - Recombinant Protein or Peptide
Result: Positive
Western Blot using CPTC-CSTB-3 as primary Ab against rAg 10098 (CSTB) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).
Background
NCI Identification Number:
10098
Antigen Name:
Cystatin B
CPTC Name:
CPTC-CSTB
Aliases:
CPI-B; CST6; EPM1; PME; STFB; Stefin-B
Function:
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins L, H and B. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy
This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B
Chromosomal Localization:
21q22.3
Expression System:
E. Coli
Accession Number:
BC010532
UniProt Accession Number:
P04080
DNA Source:
HIP : HsCD00002240
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
4470
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMMCGAPSATQPATAETQHIADQVRSQLEEKENKKFPVFKAVSFKSQV
VAGTNYFIKVHVGDEDFVHLRVFQSLPHENKPLTLSNYQTNKAKHDELTY
F
Native Sequence:
MMCGAPSATQPATAETQHIADQVRSQLEEKENKKFPVFKAVSFKSQVVAG
TNYFIKVHVGDEDFVHLRVFQSLPHENKPLTLSNYQTNKAKHDELTYF
Calculated Isoelectric Point:
6.94
Molecular Weight:
11412
Last Updated:
09/01/2008
Links
- EnsEMBL - EnsEMBL CSTB
- Entrez - Entrez CSTB
- PlasmID - CPTC-CSTB.v
- Uniprot - Uniprot P04080
Characterization Data
Gel
CPTC-CSTB SDS-PAGE
PAGE of CSTB (Ag 10098) with molecular weight standards in lane 1
SOPs
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