The Clinical Proteomic Technologies for Cancer (original) (raw)
DNA-Damage Inducible Transcript 3
Background
Catalog Number:
CPTC-DDIT3-1
RRID:
AB_10804293
Target Antigen:
DNA-Damage Inducible Transcript 3
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
11/06/2019
Antigen Recognition(s):
Purchase
- DSHB CPTC-DDIT3-1
Characterization Data [Compare Characterization Data]
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Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Western Blot - Recombinant Protein or Peptide
Click image to enlarge
Result: Positive
Western Blot using CPTC-DDIT3-1 as primary Ab against Ag 10299 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Background
Catalog Number:
CPTC-DDIT3-2
RRID:
AB_2617244
Target Antigen:
DNA-Damage Inducible Transcript 3
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
11/06/2019
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-DDIT3-2
Characterization Data [Compare Characterization Data]
Click image to enlarge
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Western Blot - Recombinant Protein or Peptide
Click image to enlarge
Result: Positive
Western Blot using CPTC-DDIT3-2 as primary Ab against Ag 10299 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Background
Catalog Number:
CPTC-DDIT3-3
Target Antigen:
DNA-Damage Inducible Transcript 3
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
11/06/2019
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-DDIT3-3
Characterization Data [Compare Characterization Data]
Click image to enlarge
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Western Blot - Recombinant Protein or Peptide
Click image to enlarge
Result: Positive
Western Blot using CPTC-DDIT3-3 as primary Ab against Ag 10299 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Background
NCI Identification Number:
10299
Antigen Name:
DNA-Damage Inducible Transcript 3
CPTC Name:
CPTC-DDIT3
Aliases:
DNA-damage-inducible transcript 3; CHOP10; GADD153; CHOP; C/EBP-homologous protein 10; Growth arrest and DNA damage-inducible protein GADD 153; DDIT-3
Function:
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP(liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and
erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
Chromosomal Localization:
12q13.1 - q13.2
Expression System:
E. Coli
Accession Number:
BC003637.1
UniProt Accession Number:
P35638
DNA Source:
DNASU - HsCD00003368
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
19480
Buffers:
PBS without Ca++ and Mg++
Expressed Sequence:
SNAMAAESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEE
ESKIFTTLDPASLAWLTEEEPEPAEVTSTSQSPHSPDSSQSSLAQEEEEE
DQGRTRKRKQSGHSPARAGKQRMKEKEQENERKVAQLAEENERLKQEIER
LTREVEATRRALIDRMVNLHQA
Native Sequence:
MAAESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEEESK
IFTTLDPASLAWLTEEEPEPAEVTSTSQSPHSPDSSQSSLAQEEEEEDQG
RTRKRKQSGHSPARAGKQRMKEKEQENERKVAQLAEENERLKQEIERLTR
EVEATRRALIDRMVNLHQA
Calculated Isoelectric Point:
4.61
Molecular Weight:
19447
Last Updated:
08/22/2020
Links
- Entrez - Entrez DDIT3
- Uniprot - Uniprot P35638
Characterization Data
SOPs
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