The Clinical Proteomic Technologies for Cancer (original) (raw)

Deleted in liver cancer 1

Background

NCI Identification Number:

00016

Antigen Name:

Deleted in liver cancer 1

CPTC Name:

CPTC-DLC1

Aliases:

Deleted In Liver Cancer 1 Variant 2; ARHGAP7; STARD12; HP; P122-RhoGAP; DLC-1; Deleted In Liver Cancer 1 Protein; Rho-Type GTPase-Activating Protein 7; START Domain-Containing Protein 12; Rho GTPase-Activating Protein 7; StAR-Related Lipid Transfer (START) Domain Containing 12; KIAA1723; StARD12; HP Protein; StAR-Related Lipid Transfer Protein 12

Function:

This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.

Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality

Chromosomal Localization:

8p22

Expression System:

E. coli

Accession Number:

NM_006094

UniProt Accession Number:

Q96QB1

DNA Source:

Mammalian Gene Collection

Immunogen:

Recombinant Full Length Protein

Vector Name:

pET28-MHL

Extinction Coefficient:

Buffers:

PBS, pH 7.4

Expressed Sequence:

mhhhhhhssgrenlyfqgSVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNH
CLDQVGLFRKSGVKSRIQALRQMNEGAIDCVNYEGQSAYDVADMLKQYFR
DLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLL
YFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSL
GKPDQKDLNENLAATQGLAHMIAECKKL

Native Sequence:

Calculated Isoelectric Point:

9.1

Molecular Weight:

26004

Last Updated:

06/17/2020

Links

• EnsEMBL - EnsEMBL DLC1
• Entrez - Entrez DLC1
• Uniprot - Uniprot Q96QB1

Characterization Data

Gel

Click image to enlarge

CPTC-DLC1 PAGE

PAGE of DLC1 (rAg 00016) with molecular weight standards in lane 1.

SOPs

• Protein Production and Purification by Structural Genome Consortium (92.9 KB)

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