The Clinical Proteomic Technologies for Cancer (original) (raw)
Fatty Acid-Binding Protein, Intestinal
Background
Catalog Number:
CPTC-FABP2-1
RRID:
AB_10658155
Target Antigen:
Fatty Acid-Binding Protein, Intestinal
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-FABP2-1
External Links
- Human Protein Atlas CPTC-FABP2-1
Characterization Data [Compare Characterization Data]
Click image to enlarge
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & actin filaments. Human assay: A-431 fixed with PFA, dilution: 1:50
Human assay: U-2 OS fixed with PFA, dilution: 1:50
Human assay: U-251 MG fixed with PFA, dilution: 1:50
Click image to enlarge
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Western Blot - Recombinant Protein or Peptide
Click image to enlarge
Result: Positive
Western Blot using CPTC-FABP2-1 as primary Ab against rAg 10977 (FABP2) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).
Background
Catalog Number:
CPTC-FABP2-2
RRID:
AB_10660831
Target Antigen:
Fatty Acid-Binding Protein, Intestinal
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-FABP2-2
External Links
- Human Protein Atlas CPTC-FABP2-2
Characterization Data [Compare Characterization Data]
Click image to enlarge
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & actin filaments. Human assay: A-431 fixed with PFA, dilution: 1:125
Human assay: U-2 OS fixed with PFA, dilution: 1:125
Human assay: U-251 MG fixed with PFA, dilution: 1:125
Click image to enlarge
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Western Blot - Recombinant Protein or Peptide
Click image to enlarge
Result: Positive
Western Blot using CPTC-FABP2-2 as primary Ab against rAg 10977 (FABP2) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as control for goat anti-mouse HRP secondary binding (lane 3).
Background
NCI Identification Number:
10977
Antigen Name:
Fatty Acid-Binding Protein, Intestinal
CPTC Name:
CPTC-FABP2
Aliases:
FABPI; I-FABP; MGC133132
Function:
The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids.
They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis.
Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
Chromosomal Localization:
4q28 - q31
Expression System:
E. Coli
Accession Number:
BC069625.1
UniProt Accession Number:
P12104
DNA Source:
OB : MHS1010-98684774
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
16960
Buffers:
PBS
Expressed Sequence:
SNAMAFDSTWKVDRSENYDKFMEKMGVNIVKRKLAAHDNLKLTITQEGNK
FTVKESSAFRNIEVVFELGVTFNYNLADGTELRGTWSLEGNKLIGKFKRT
DNGNELNTVREIIGDELVQTYVYEGVEAKRIFKKD
Native Sequence:
MAFDSTWKVDRSENYDKFMEKMGVNIVKRKLAAHDNLKLTITQEGNKFTV
KESSAFRNIEVVFELGVTFNYNLADGTELRGTWSLEGNKLIGKFKRTDNG
NELNTVREIIGDELVQTYVYEGVEAKRIFKKD
Calculated Isoelectric Point:
6.62
Molecular Weight:
15.21
Last Updated:
08/22/2020
Links
- EnsEMBL - EnsEMBL FABP2
- Entrez - Entrez FABP2
- Uniprot - Uniprot P12104
Characterization Data
Gel
Click image to enlarge
CPTC-FABP2 PAGE
PAGE of Ag 10977 (with molecular weight standards in lane 1)
SOPs
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