The Clinical Proteomic Technologies for Cancer (original) (raw)
Geminin
Background
Catalog Number:
CPTC-GMNN-1
RRID:
AB_2617263
Target Antigen:
Geminin
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
05/23/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-GMNN-1
External Links
- Human Protein Atlas CPTC-GMNN-1
Characterization Data [Compare Characterization Data]
Result: Positive
Imaging mass cytometry on normal kidney tissue core using CPTC-GMNN-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, placenta, prostate, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidney) and cancer tissues (colon, breast, ovarian, lung, and prostate).
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Western Blot - Recombinant Protein or Peptide
Result: Positive
Western Blot using CPTC-GMNN-1 as primary Ab against GMNN (Ag 10987) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Background
Catalog Number:
CPTC-GMNN-2
RRID:
AB_10805876
Target Antigen:
Geminin
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
05/23/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-GMNN-2
External Links
- Human Protein Atlas CPTC-GMNN-2
Characterization Data [Compare Characterization Data]
Result: Positive
Imaging mass cytometry on normal lung tissue core using CPTC-GMNN-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, placenta, prostate, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidney) and cancer tissues (colon, breast, ovarian, lung, and prostate).
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Human assay: A-431 fixed with PFA, dilution: 1:200
Human assay: U-2 OS fixed with PFA, dilution: 1:200
Human assay: U-251 MG fixed with PFA, dilution: 1:200
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Western Blot - Recombinant Protein or Peptide
Result: Positive
Western Blot using CPTC-GMNN-2 as primary Ab against GMNN (Ag 10987) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Background
Catalog Number:
CPTC-GMNN-3
Target Antigen:
Geminin
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
11/14/2019
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-GMNN-3
Characterization Data [Compare Characterization Data]
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Western Blot - Recombinant Protein or Peptide
Result: Positive
Western Blot using CPTC-GMNN-3 as primary Ab against GMNN (Ag 10987) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Background
NCI Identification Number:
10987
Antigen Name:
Geminin
CPTC Name:
CPTC-GMNN
Aliases:
Gem; OTTHUMP00000039393; RP3-369A17.3; geminin
Function:
Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
Chromosomal Localization:
6p22.2
Expression System:
E. Coli
Accession Number:
BC005185.1
UniProt Accession Number:
O75496
DNA Source:
OB : MHS1011-62496
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
10033
Buffers:
PBS
Expressed Sequence:
SNAMNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELSAG
LSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQ
YWKEVAEKRRKALYEALKENEKLHKEIEQKDNEIARLKKENKELAEVAEH
VQYMAELIERLNGEPLDNFESLDNQEFDSEEETVEDSLVEDSEIGTCAEG
TVSSSTDAKPCI
Native Sequence:
MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELSAGLSK
RKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWK
EVAEKRRKALYEALKENEKLHKEIEQKDNEIARLKKENKELAEVAEHVQY
MAELIERLNGEPLDNFESLDNQEFDSEEETVEDSLVEDSEIGTCAEGTVS
SSTDAKPCI
Calculated Isoelectric Point:
4.93
Molecular Weight:
23.57
Last Updated:
12/11/2009
Links
- EnsEMBL - EnsEMBL GMNN
- Entrez - Entrez GMNN
- Uniprot - Uniprot O75496
Characterization Data
Gel
CPTC-GMNN PAGE
PAGE of Ag 10987 (with molecular weight standards in lane 1)
SOPs
Don't have Adobe Reader™?
Get it for free at Adobe.com