The Clinical Proteomic Technologies for Cancer (original) (raw)

Mucin 1, cell surface associated Peptide 2

Background

Catalog Number:

CPTC-MUC1-2

RRID:

AB_2617292

Target Antigen:

Mucin 1, cell surface associated Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide

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Characterization Data [Compare Characterization Data]

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Background

NCI Identification Number:

00065

Antigen Name:

Mucin 1, cell surface associated Peptide 2

CPTC Name:

CPTC-MUC1 Peptide 2

Aliases:

Mucin 1, Cell Surface Associated; PUM; PEM; CD227; Mucin 1, Transmembrane; Breast Carcinoma-Associated Antigen DF3; Cancer Antigen 15-3; Carcinoma-Associated Mucin; Krebs Von Den Lungen-6; Peanut-Reactive Urinary Mucin; Polymorphic Epithelial Mucin; Tumor-Associated Epithelial Membrane Antigen; Tumor-Associated Mucin; EMA; H23AG; KL-6; MUC-1; PEMT; CA 15-3; MAM6; MUC-1/SEC; MUC-1/X; MUC1/ZD; DF3 Antigen; Episialin; H23 Antigen; Mucin-1; Tumor Associated Epithelial Mucin; CD227 Antigen

Function:

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant
intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This
gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May
provide a protective layer on epithelial cells against bacterial and enzyme attack.

The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations
and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells,
influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated
transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

Chromosomal Localization:

1q21

Accession Number:

NP_002447.4.

UniProt Accession Number:

P15941

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

NYGQLDIFPAR

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1210

Last Updated:

10/30/2013

Characterization Data

SOPs

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