The Clinical Proteomic Technologies for Cancer (original) (raw)

Nucleoside Diphosphate Kinase B

Background

NCI Identification Number:

10385

Antigen Name:

Nucleoside Diphosphate Kinase B

CPTC Name:

CPTC-NME2

Aliases:

Nucleoside Diphosphate Kinase B; NME2; NM23B; EC2.7.4.6; MGC111212; NDPKB; NM23-H2

Function:

Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC.

Acts as a transcriptional activator of the c-Myc gene; binds DNA nonspecifically

Chromosomal Localization:

17q21 - q22

Expression System:

E. Coli

Accession Number:

BC002476

UniProt Accession Number:

P22392

DNA Source:

HIP:HsCD00002962

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

22523

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEH
LKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPA
DSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAH
DWVYE

Native Sequence:

MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQ
HYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSK
PGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWV
YE

Calculated Isoelectric Point:

8.52

Molecular Weight:

17570

Last Updated:

01/11/2021

Links

• EnsEMBL - EnsEMBL NME2
• Uniprot - Uniprot P22392

Characterization Data

Gel

Click image to enlarge

PAGE of ANL antigen 10385

Electrophoresis of antigen. Lane 1 has molecular weight markers, lane 2 has the antigen.

SOPs

• BL21 Competant Cells (25.5 KB)

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