The Clinical Proteomic Technologies for Cancer (original) (raw)

RAD9 Checkpoint Clamp Component A Peptide 1

Background

Catalog Number:

CPTC-RAD9A-1

Target Antigen:

RAD9 Checkpoint Clamp Component A Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

02/16/2024

Antigen Recognition(s):

Peptide, Phosphorylation

Characterization Data [Compare Characterization Data]

Western Blot - Tissue or Cell Lysate

Click to enlarge image Western blot using CPTC-RAD9A-1 as primary antibody against LCL57 cell lysate. Cell lysate was irradiated with 10 Gy as shown in the ‘+’ indicated lane. Non-irradiated cell lysate was treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lane. Blots were developed using enhanced chemiluminescence. Molecular weight standards are also included. Click image to enlarge

Result: Negative

Western blot using CPTC-RAD9A-1 as primary antibody against LCL57 cell lysate. Cell lysate was irradiated with 10 Gy as shown in the ‘+’ indicated lane. Non-irradiated cell lysate was treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lane. Blots were developed using enhanced chemiluminescence. Molecular weight standards are also included.

Background

NCI Identification Number:

00320

Antigen Name:

RAD9 Checkpoint Clamp Component A Peptide 1

CPTC Name:

CPTC-RAD9A Peptide 1

Aliases:

RAD9 Checkpoint Clamp Component A; Cell Cycle Checkpoint Control Protein RAD9A; DNA Repair Exonuclease Rad9 Homolog A; HRAD9; RAD9 Homolog A (S. Pombe); RAD9 (S. Pombe) Homolog; RAD9 Homolog A; EC 3.1.11.2; RAD9

Function:

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. RAD9A (RAD9 Checkpoint Clamp Component A) is a Protein Coding gene. Diseases associated with RAD9A include Alpha-Thalassemia/Mental Retardation Syndrome, X-Linked and Spinocerebellar Ataxia Type 1 With Axonal Neuropathy. Among its related pathways are E2F mediated regulation of DNA replication and DNA Double-Strand Break Repair. Gene Ontology (GO) annotations related to this gene include protein kinase binding and SH3 domain binding. An important paralog of this gene is RAD9B.Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Chromosomal Localization:

11q13.2

Accession Number:

NP_001230153.1

UniProt Accession Number:

Q99638

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

SPQGPSPVLAEDS(ph)EGEG

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1870

Last Updated:

02/19/2020

Characterization Data

SOPs

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