The Clinical Proteomic Technologies for Cancer (original) (raw)
Serpin Peptidase Inhibitor, Clade A, Member 1
Background
Catalog Number:
CPTC-SERPINA1-1
RRID:
AB_2617341
Target Antigen:
Serpin Peptidase Inhibitor, Clade A, Member 1
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
05/28/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-SERPINA1-1
External Links
- Human Protein Atlas CPTC-SERPINA1-1
Characterization Data [Compare Characterization Data]
Result: Positive
Imaging mass cytometry on normal lung tissue core using CPTC-SERPINA1-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, placenta, prostate, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidney) and cancer tissues (breast, colon, ovarian, lung, and prostate).
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Positive
Protein Array in which CPTC-SERPINA1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Western Blot - Recombinant Protein or Peptide
Result: Positive
Western Blot using CPTC-SERPINA1-1 as primary Ab against SERPINA1 (rAg 11019) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Background
NCI Identification Number:
11019
Antigen Name:
Serpin Peptidase Inhibitor, Clade A, Member 1
CPTC Name:
CPTC-SERPINA1
Aliases:
AAT; PI; PI1; alpha1AT1; alpha-1-antitrypsin1; A1AT; A1A; Serpin A1; Alpha-1 protease inhibitor; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase,
antitrypsin), member 1; OTTHUMP00000197154; MGC23330; OTTHUMP00000197150; PRO2275; MGC9222; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; alpha-1-antiproteinase
Function:
The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver
disease. Several transcript variants encoding the same protein have been found for this gene.
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for
plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form
inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT (SPAAT) is a reversible chymotrypsin inhibitor. It also inhibits elastase, but not
trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic
destruction by human leukocyte elastase (HLE).
Chromosomal Localization:
14q32.1
Expression System:
E. Coli
Accession Number:
BC015642.2
UniProt Accession Number:
P01009
DNA Source:
OpenBiosystems - MHS1011-76439
Immunogen:
Recombinant Full Length Protein
Vector Name:
xx
Extinction Coefficient:
19940
Buffers:
PBS without Ca++ and Mg++
Expressed Sequence:
SNAEDPQGDAAQKTDTSHHDQDHPTFNKITPNLAEFAFSLYRQLAHQSNS
TNIFFSPVSIATAFAMLSLGTKADTHDEILEGLNFNLTEIPEAQIHEGFQ
ELLRTLNQPDSQLQLTTGNGLFLSEGLKLVDKFLEDVKKLYHSEAFTVNF
GDTEEAKKQINDYVEKGTQGKIVDLVKELDRDTVFALVNYIFFKGKWERP
FEVKDTEEEDFHVDQATTVKVPMMKRLGMFNIQHCKKLSSWVLLMKYLGN
ATAIFFLPDEGKLQHLENELTHDIITKFLENEDRRSASLHLPKLSITGTY
DLKSVLGQLGITKVFSNGADLSGVTEEAPLKLSKAVHKAVLTIDEKGTEA
AGAMFLEAIPMSIPPEVKFNKPFVFLMIEQNTKSPLFMGKVVNPTQK
Native Sequence:
MPSSVSWGILLLAGLCCLVPVSLAEDPQGDAAQKTDTSHHDQDHPTFNKI
TPNLAEFAFSLYRQLAHQSNSTNIFFSPVSIATAFAMLSLGTKADTHDEI
LEGLNFNLTEIPEAQIHEGFQELLRTLNQPDSQLQLTTGNGLFLSEGLKL
VDKFLEDVKKLYHSEAFTVNFGDTEEAKKQINDYVEKGTQGKIVDLVKEL
DRDTVFALVNYIFFKGKWERPFEVKDTEEEDFHVDQVTTVKVPMMKRLGM
FNIQHCKKLSSWVLLMKYLGNATAIFFLPDEGKLQHLENELTHDIITKFL
ENEDRRSASLHLPKLSITGTYDLKSVLGQLGITKVFSNGADLSGVTEEAP
LKLSKAVHKAVLTIDEKGTEAAGAMFLEAIPMSIPPEVKFNKPFVFLMIE
QNTKSPLFMGKVVNPTQK
Calculated Isoelectric Point:
5.37
Molecular Weight:
44569
Last Updated:
11/19/2010
Links
- Entrez - Entrez SERPINA1
- Uniprot - Uniprot P01009
Characterization Data
Gel
CPTC-SERPINA1 PAGE
PAGE of Ag 11019 (with molecular weight standards in lane 1)
SOPs
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