The Clinical Proteomic Technologies for Cancer (original) (raw)

Telomeric Repeat Binding Factor 2 Peptide 2

Background

NCI Identification Number:

00207

Antigen Name:

Telomeric Repeat Binding Factor 2 Peptide 2

CPTC Name:

CPTC-TERF2 Peptide 2

Aliases:

Telomeric Repeat Binding Factor 2; TRBF2; TRF2; Telomeric DNA-Binding Protein; TTAGGG Repeat-Binding Factor 2; Telomeric Repeat Binding Protein 2

Function:

This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic.

TERF2 (telomeric repeat binding factor 2) is a protein-coding gene. GO annotations related to this gene include protein C-terminus binding and protein homodimerization activity. An important paralog of this gene is TERF1.

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Chromosomal Localization:

16q22.1

Accession Number:

NP_005643.2

UniProt Accession Number:

Q15554

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

x

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1760

Last Updated:

07/05/2024

Characterization Data

SOPs

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