The Clinical Proteomic Technologies for Cancer (original) (raw)
Ubiquitin-like modifier activating enzyme 1
Background
Catalog Number:
CPTC-UBA1-1
Target Antigen:
Ubiquitin-like modifier activating enzyme 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-UBA1-1
External Links
- Human Protein Atlas CPTC-UBA1-1
Characterization Data [Compare Characterization Data]
Background
Catalog Number:
CPTC-UBA1-2
RRID:
AB_2617365
Target Antigen:
Ubiquitin-like modifier activating enzyme 1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-UBA1-2
Characterization Data [Compare Characterization Data]
Background
Catalog Number:
CPTC-UBA1-3
RRID:
AB_2617366
Target Antigen:
Ubiquitin-like modifier activating enzyme 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-UBA1-3
Characterization Data [Compare Characterization Data]
Background
NCI Identification Number:
00057
Antigen Name:
Ubiquitin-like modifier activating enzyme 1
CPTC Name:
CPTC-UBA1
Aliases:
UBE1; A1S9T; GXP1; POC20; UBE1X; Ubiquitin-Activating Enzyme E1; A1ST; AMCX1; SMAX2; A1S9; UBA1A; A1S9T And BN75 Temperature Sensitivity Complementing; POC20 Centriolar Protein Homolog; UBA1, Ubiquitin-Activating Enzyme E1 Homolog A; Ubiquitin-Like Modifier-Activating Enzyme 1; Protein A1S9
Function:
The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
Chromosomal Localization:
XP11.23
Expression System:
E. coli
Accession Number:
NP_695012.1
UniProt Accession Number:
P22314
DNA Source:
mammalian Gene Collection
Immunogen:
Recombinant Full Length Protein
Vector Name:
pET 28-MHL
Extinction Coefficient:
Buffers:
PBS pH 7.4
Expressed Sequence:
mhhhhhhssgrenlyfqgGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVL
VSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKN
RAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVG
EFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTK
DNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFS
ICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRP
AQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQN
NLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYF
DALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGA
GAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTK
LKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALD
NVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPE
KSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERT
LRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLL
HNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTY
GLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRL
EELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIP
SADRHKSKLIAG
Native Sequence:
Calculated Isoelectric Point:
5.2
Molecular Weight:
95916
Last Updated:
10/23/2013
Links
- Entrez - Entrez UBA1
- EnsEMBL - EnsEMBL UBA1
- Uniprot - Uniprot P22314
Characterization Data
Gel
Click image to enlarge
CPTC-UBA1 PAGE
PAGE of UBA1 (rAg 00057) with molecular weight standards in lane 1
SOPs
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