Mykola Ovcharenko | Akademia Pomorska w Słupsku - Pomeranian University in Słupsk (original) (raw)
Papers by Mykola Ovcharenko
Wiadomości Parazytologiczne, 1998
HAL (Le Centre pour la Communication Scientifique Directe), Aug 8, 2023
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Widespread infection, diversification and old host associations of Nosema Microsporidia in European freshwater gammarids (Amphipoda).
Authorea (Authorea), Apr 27, 2023
The major current challenge related to alien species is the absence of a comprehensive theoretica... more The major current challenge related to alien species is the absence of a comprehensive theoretical basis to explain the resistance from bioinvasions. One of the factors conferring resistance to invasion is the native species diversity of a recipient ecosystem. We aim to examine patterns of species diversity to understand the mechanisms underlying host-parasite relationships in alien species. The dataset represents 20 host-locality samples, including over 15,067,600 symbiotic organisms counted from 612 host individuals belonging to 7 gammarid species, two native and five invasive, sampled at 16 localities in the Baltic region of Poland. We asked whether diversity patterns depended on locality, habitat and host species, and which factors are deterministic in the variation of diversity. Our results suggest that both locality and host phylogeny are critical factors in determining the composition and abundance of symbiotic communities in gammarids. Out of these two factors, locality appears to be more determinant of the symbiotic community of gammarids than host species, whereas habitat condition was a stronger determinant of the symbiotic communities than geographic distance. The rich and uneven symbiotic communities of native gammarids keep the host-parasite system in equilibrium. Sharply dominated symbiotic species with a high load per host individual stabilize the host population. Simplified symbiotic communities of the invasive hosts showed a relatively even distribution of species with moderate loads per individual, with heavily infected gammarids being rare in the population. The survival rate of invasive gammarids is expected to be relatively high, facilitating their population growth and further spread. Complex communities of the native gammarids constructed from many symbiotic species may prevent host populations from undergoing explosive growth, while such mechanisms may be completely or partially reduced in invasive hosts.
zoodiversity
The diversity and taxonomy of metacercariae infecting freshwater amphipods of Poland are predomin... more The diversity and taxonomy of metacercariae infecting freshwater amphipods of Poland are predominantly poorly known. During parasitological surveys of Gammarus pulex (Crustacea: Amphipoda) in the Pomeranian region of Poland in 2020 and 2021, some specimens of crustaceans were found to harbour metacercariae. Out of seven observed localities, metacercariae were found in the stream close to Krępa Słupska and the Lupawa River close to Smoldzino. They were morphologically studied and sequenced using universal eukaryotic primers that amplify V4–V5 regions of 18S rRNA. The BLAST analysis and phylogenetic reconstructions aid to define the affiliation of the revealed metacercariae within the Omphalometridae Looss, 1899. The further differential analysis based on morphologic information led to the conclusion that the metacercarial form studied herein represents Neoplagioglyphe megastomus (Baer, 1943). The degree of morphometric variations of taxonomic important features in populations from di...
International Journal for Parasitology
Seasonal observations of prevalence and intensity of microsporidians and gregarines infecting the... more Seasonal observations of prevalence and intensity of microsporidians and gregarines infecting the native amphipod Gammarus pulex, in the tributary of small Baltic coastal river were analyzed. Using aggregate data from 12 date-by-site comparisons, we found that both prevalence and intensity of Apicomplexa were seasonally unimodal. Parasitism first increased and then declined seasonally after peaking midseason. In the case of infections caused by Microsporidia, the highest increase falls in the early autumn period, reaching the value from 48 to 56%. The minimum share of infected hosts was recorded in March 2016 and January 2017. In the early spring period, there was a slight decrease in microsporidian infection with subsequent minor fluctuations in the summer. We showed that this parasite can decreases the survival of G. pulex in the late stages of infection development in autumn. However, the host reproduction in the spring and the summer could potentially reduce the negative impact ...
Fishes
A subpopulation of 97 European eels, Anguilla anguilla, was obtained from a local consortium of L... more A subpopulation of 97 European eels, Anguilla anguilla, was obtained from a local consortium of Lake Trasimeno. The fish were examined for parasites in the swimbladder (SB) and intestine; the SBs of 66 (68%) of the 97 eels contained 480 adult specimens of the nematode Anguillicoloides crassus (Kuwahara, Niimi and Itagaki, 1974) and the intensity of infection ranged from 1 to 18 adult worms per SB (7.27 ± 0.43, mean ± SE). In heavily infected SB, the wall was thicker; upon excision, black-brownish adult nematodes were noticed inside the lumen. The infected SBs showed a papillose aspect of the epithelium and frequent erosion, inflammation, hemorrhages, and dilation of blood vessels. In parasitized SBs, mast cells and macrophages were encountered in the mucosal layer; in several heavily infected SB, rodlet cells were the sole fish immune cells noticed in the epithelium and in close contact with the A. crassus. With reference to the eel intestinal epithelium, in fifteen eels, two develo...
Acta Parasitologica, 2001
Knowledge and Management of Aquatic Ecosystems, 2014
A parasitological study of Ponto-Caspian gobies, including the monkey goby Neogobius fluviatilis,... more A parasitological study of Ponto-Caspian gobies, including the monkey goby Neogobius fluviatilis, racer goby Babka gymnotrachelus and tubenose goby Proterorhinus semilunaris, carried out over four years in the lower Vistula River is described. These fish species represent one of the most impressive invasions of European inland waters, connected with the spontaneous, east-to-west intracontinental movement observed in the last two decades. The parasite community consisted of 24 taxa. Typical for racer goby were: Trichodina domerguei, Diplostomum pseudospathaceum, Gyrodactylus proterorhini and glochidia of unionids. The list of parasites typical for monkey goby is supplemented with: Tylodelphys clavata (met.) and Eimeria sp., while the glochidia were rarely detected in this fish host. Holostephanus spp., Apatemon gracilis, Diplostomum gobiorum and glochidia predominated in parasite fauna of tubenose goby. Unlike the other species tested, P. semilunaris was poorly infected with T. domerguei and G. proterorhini. Parasites commonly distributed through native fishes in the observed area prevailed in gobies. Species rare in natives were also numerously represented. In this way, alien fish reinforce populations of those parasites in invaded waters. Parasites dragged to the colonized area (G. proterorhini, Holostephanus spp., A. gracilis and D. gobiorum) complement the community. Larval stages were typical for parasite fauna of all studied gobies. RÉSUMÉ Parasites des gobies non indigènes dans le réservoir Włocławek sur la partie inférieure du fleuve Vistule, première étude exhaustive en Pologne Mots-clés : espèces L'étude parasitologique des gobies Ponto-Caspiens, comprenant le gobie fluviatile Neogobius fluviatilis, le gobie coureur Babka gymnotrachelus et le gobie demielune Proterorhinus semilunaris réalisée sur quatre ans dans le cours inférieur du
Limnological Papers, 2008
In total, we analyzed over 4000 individuals of 12 gammarid species from the deltaic system of the... more In total, we analyzed over 4000 individuals of 12 gammarid species from the deltaic system of the Vistula River, Vistula Lagoon, littoral of the Baltic Sea near Dqbki, as well as from small rivers draining directly to the sea in this area. Gregarines (Apicomplexa, Gregarinidae) were recorded in digestive tracks of invasive Pontogammarus robustoides (Uradiophora ramosa (Balcescu-Codreanu 1974) and Cephaloidophora mucronata (Codreanu-Balcescu 1995)) from the Vistula deltaic system, and in digestive system of native Gammarus pulex (Cephaloidophora gammari (Franzius 1848)) from Stradanka river, an affluent of the Vistula Lagoon. Microsporidia were found also only in the two above gammarid species: Pleistophora muelleri (Pfeiffer 1895) in G. pulex from Piainica river, and Nosemapontogammari (Ovcharenko and Kurandina 1987) from the Vistula deltaic system. All the above microparasites are new to Poland.
Sampling protocol of grey mullet parasites agreed by all participants in the project "Evalua... more Sampling protocol of grey mullet parasites agreed by all participants in the project "Evaluating the effect of an invasive species on local mullet communities in the Mediterranean: A parasite community approach".
Ukrains'kyi Visnyk Psykhonevrolohii, 2021
The purpose of the work is to study individual manifestations of adverse effects of drinking peop... more The purpose of the work is to study individual manifestations of adverse effects of drinking people on adults in their microsocial environment. In four regions of Ukraine (Kharkiv, Luhansk and Zaporizhzhia regions, as well as the city of Kyiv), during 2018—2020, 1742 people were examined, who belonged to three qualitatively different comparison groups: patients with alcohol dependence (393 people); healthy relatives of alcohol-dependent patients (274 people) and representatives of the general population (1075 people). Of these surveyed, respondents were identified who had drinkers in the environment: 481 people among healthy people and 69 people among patients with alcohol dependence. Further research focused on this particular contingent. The main research tool was the questionnaire of the international research consortium GENAHTO (Gender, Alcohol, and Harms to Others). The obtained data were processed by methods of mathematical statistics (variance and correlation analysis) on a c...
Ukrains'kyi Visnyk Psykhonevrolohii, 2021
The purpose of the work is to study individual manifestations of adverse effects of drinking peop... more The purpose of the work is to study individual manifestations of adverse effects of drinking people on children in their microsocial environment. In four regions of Ukraine (Kharkiv, Luhansk and Zaporizhzhia regions, as well as the city of Kyiv), during 2018—2020, 1742 people were examined, who belonged to three qualitatively different comparison groups: patients with alcohol dependence (AD) (393 people); healthy relatives of alcohol-dependent patients (274 people) and representatives of the general population (1075 people). Of these surveyed, respondents were identified who had children in the environment: 288 persons among healthy people and 121 persons among patients with AD). Further research focused on this particular contingent. The main research tool was the questionnaire of the international research consortium GENAHTO (Gender, Alcohol, and Harms to Others). The obtained data were processed by methods of mathematical statistics (variance and correlation analysis) on a comput...
Wiadomości Parazytologiczne, 1998
HAL (Le Centre pour la Communication Scientifique Directe), Aug 8, 2023
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Widespread infection, diversification and old host associations of Nosema Microsporidia in European freshwater gammarids (Amphipoda).
Authorea (Authorea), Apr 27, 2023
The major current challenge related to alien species is the absence of a comprehensive theoretica... more The major current challenge related to alien species is the absence of a comprehensive theoretical basis to explain the resistance from bioinvasions. One of the factors conferring resistance to invasion is the native species diversity of a recipient ecosystem. We aim to examine patterns of species diversity to understand the mechanisms underlying host-parasite relationships in alien species. The dataset represents 20 host-locality samples, including over 15,067,600 symbiotic organisms counted from 612 host individuals belonging to 7 gammarid species, two native and five invasive, sampled at 16 localities in the Baltic region of Poland. We asked whether diversity patterns depended on locality, habitat and host species, and which factors are deterministic in the variation of diversity. Our results suggest that both locality and host phylogeny are critical factors in determining the composition and abundance of symbiotic communities in gammarids. Out of these two factors, locality appears to be more determinant of the symbiotic community of gammarids than host species, whereas habitat condition was a stronger determinant of the symbiotic communities than geographic distance. The rich and uneven symbiotic communities of native gammarids keep the host-parasite system in equilibrium. Sharply dominated symbiotic species with a high load per host individual stabilize the host population. Simplified symbiotic communities of the invasive hosts showed a relatively even distribution of species with moderate loads per individual, with heavily infected gammarids being rare in the population. The survival rate of invasive gammarids is expected to be relatively high, facilitating their population growth and further spread. Complex communities of the native gammarids constructed from many symbiotic species may prevent host populations from undergoing explosive growth, while such mechanisms may be completely or partially reduced in invasive hosts.
zoodiversity
The diversity and taxonomy of metacercariae infecting freshwater amphipods of Poland are predomin... more The diversity and taxonomy of metacercariae infecting freshwater amphipods of Poland are predominantly poorly known. During parasitological surveys of Gammarus pulex (Crustacea: Amphipoda) in the Pomeranian region of Poland in 2020 and 2021, some specimens of crustaceans were found to harbour metacercariae. Out of seven observed localities, metacercariae were found in the stream close to Krępa Słupska and the Lupawa River close to Smoldzino. They were morphologically studied and sequenced using universal eukaryotic primers that amplify V4–V5 regions of 18S rRNA. The BLAST analysis and phylogenetic reconstructions aid to define the affiliation of the revealed metacercariae within the Omphalometridae Looss, 1899. The further differential analysis based on morphologic information led to the conclusion that the metacercarial form studied herein represents Neoplagioglyphe megastomus (Baer, 1943). The degree of morphometric variations of taxonomic important features in populations from di...
International Journal for Parasitology
Seasonal observations of prevalence and intensity of microsporidians and gregarines infecting the... more Seasonal observations of prevalence and intensity of microsporidians and gregarines infecting the native amphipod Gammarus pulex, in the tributary of small Baltic coastal river were analyzed. Using aggregate data from 12 date-by-site comparisons, we found that both prevalence and intensity of Apicomplexa were seasonally unimodal. Parasitism first increased and then declined seasonally after peaking midseason. In the case of infections caused by Microsporidia, the highest increase falls in the early autumn period, reaching the value from 48 to 56%. The minimum share of infected hosts was recorded in March 2016 and January 2017. In the early spring period, there was a slight decrease in microsporidian infection with subsequent minor fluctuations in the summer. We showed that this parasite can decreases the survival of G. pulex in the late stages of infection development in autumn. However, the host reproduction in the spring and the summer could potentially reduce the negative impact ...
Fishes
A subpopulation of 97 European eels, Anguilla anguilla, was obtained from a local consortium of L... more A subpopulation of 97 European eels, Anguilla anguilla, was obtained from a local consortium of Lake Trasimeno. The fish were examined for parasites in the swimbladder (SB) and intestine; the SBs of 66 (68%) of the 97 eels contained 480 adult specimens of the nematode Anguillicoloides crassus (Kuwahara, Niimi and Itagaki, 1974) and the intensity of infection ranged from 1 to 18 adult worms per SB (7.27 ± 0.43, mean ± SE). In heavily infected SB, the wall was thicker; upon excision, black-brownish adult nematodes were noticed inside the lumen. The infected SBs showed a papillose aspect of the epithelium and frequent erosion, inflammation, hemorrhages, and dilation of blood vessels. In parasitized SBs, mast cells and macrophages were encountered in the mucosal layer; in several heavily infected SB, rodlet cells were the sole fish immune cells noticed in the epithelium and in close contact with the A. crassus. With reference to the eel intestinal epithelium, in fifteen eels, two develo...
Acta Parasitologica, 2001
Knowledge and Management of Aquatic Ecosystems, 2014
A parasitological study of Ponto-Caspian gobies, including the monkey goby Neogobius fluviatilis,... more A parasitological study of Ponto-Caspian gobies, including the monkey goby Neogobius fluviatilis, racer goby Babka gymnotrachelus and tubenose goby Proterorhinus semilunaris, carried out over four years in the lower Vistula River is described. These fish species represent one of the most impressive invasions of European inland waters, connected with the spontaneous, east-to-west intracontinental movement observed in the last two decades. The parasite community consisted of 24 taxa. Typical for racer goby were: Trichodina domerguei, Diplostomum pseudospathaceum, Gyrodactylus proterorhini and glochidia of unionids. The list of parasites typical for monkey goby is supplemented with: Tylodelphys clavata (met.) and Eimeria sp., while the glochidia were rarely detected in this fish host. Holostephanus spp., Apatemon gracilis, Diplostomum gobiorum and glochidia predominated in parasite fauna of tubenose goby. Unlike the other species tested, P. semilunaris was poorly infected with T. domerguei and G. proterorhini. Parasites commonly distributed through native fishes in the observed area prevailed in gobies. Species rare in natives were also numerously represented. In this way, alien fish reinforce populations of those parasites in invaded waters. Parasites dragged to the colonized area (G. proterorhini, Holostephanus spp., A. gracilis and D. gobiorum) complement the community. Larval stages were typical for parasite fauna of all studied gobies. RÉSUMÉ Parasites des gobies non indigènes dans le réservoir Włocławek sur la partie inférieure du fleuve Vistule, première étude exhaustive en Pologne Mots-clés : espèces L'étude parasitologique des gobies Ponto-Caspiens, comprenant le gobie fluviatile Neogobius fluviatilis, le gobie coureur Babka gymnotrachelus et le gobie demielune Proterorhinus semilunaris réalisée sur quatre ans dans le cours inférieur du
Limnological Papers, 2008
In total, we analyzed over 4000 individuals of 12 gammarid species from the deltaic system of the... more In total, we analyzed over 4000 individuals of 12 gammarid species from the deltaic system of the Vistula River, Vistula Lagoon, littoral of the Baltic Sea near Dqbki, as well as from small rivers draining directly to the sea in this area. Gregarines (Apicomplexa, Gregarinidae) were recorded in digestive tracks of invasive Pontogammarus robustoides (Uradiophora ramosa (Balcescu-Codreanu 1974) and Cephaloidophora mucronata (Codreanu-Balcescu 1995)) from the Vistula deltaic system, and in digestive system of native Gammarus pulex (Cephaloidophora gammari (Franzius 1848)) from Stradanka river, an affluent of the Vistula Lagoon. Microsporidia were found also only in the two above gammarid species: Pleistophora muelleri (Pfeiffer 1895) in G. pulex from Piainica river, and Nosemapontogammari (Ovcharenko and Kurandina 1987) from the Vistula deltaic system. All the above microparasites are new to Poland.
Sampling protocol of grey mullet parasites agreed by all participants in the project "Evalua... more Sampling protocol of grey mullet parasites agreed by all participants in the project "Evaluating the effect of an invasive species on local mullet communities in the Mediterranean: A parasite community approach".
Ukrains'kyi Visnyk Psykhonevrolohii, 2021
The purpose of the work is to study individual manifestations of adverse effects of drinking peop... more The purpose of the work is to study individual manifestations of adverse effects of drinking people on adults in their microsocial environment. In four regions of Ukraine (Kharkiv, Luhansk and Zaporizhzhia regions, as well as the city of Kyiv), during 2018—2020, 1742 people were examined, who belonged to three qualitatively different comparison groups: patients with alcohol dependence (393 people); healthy relatives of alcohol-dependent patients (274 people) and representatives of the general population (1075 people). Of these surveyed, respondents were identified who had drinkers in the environment: 481 people among healthy people and 69 people among patients with alcohol dependence. Further research focused on this particular contingent. The main research tool was the questionnaire of the international research consortium GENAHTO (Gender, Alcohol, and Harms to Others). The obtained data were processed by methods of mathematical statistics (variance and correlation analysis) on a c...
Ukrains'kyi Visnyk Psykhonevrolohii, 2021
The purpose of the work is to study individual manifestations of adverse effects of drinking peop... more The purpose of the work is to study individual manifestations of adverse effects of drinking people on children in their microsocial environment. In four regions of Ukraine (Kharkiv, Luhansk and Zaporizhzhia regions, as well as the city of Kyiv), during 2018—2020, 1742 people were examined, who belonged to three qualitatively different comparison groups: patients with alcohol dependence (AD) (393 people); healthy relatives of alcohol-dependent patients (274 people) and representatives of the general population (1075 people). Of these surveyed, respondents were identified who had children in the environment: 288 persons among healthy people and 121 persons among patients with AD). Further research focused on this particular contingent. The main research tool was the questionnaire of the international research consortium GENAHTO (Gender, Alcohol, and Harms to Others). The obtained data were processed by methods of mathematical statistics (variance and correlation analysis) on a comput...