Ehab A Sarhan | Agricultural Research Center (Egypt) (original) (raw)

Papers by Ehab A Sarhan

Egyptian Journal of Phytopathology, 2018

Journal of Plant Protection and Pathology, 2020

Current Science International, 2019

Powdery mildew disease, Podosphaera xanthii (syn. Sphaerotheca fuliginea auct. p.p.), attacked al... more Powdery mildew disease, Podosphaera xanthii (syn. Sphaerotheca fuliginea auct. p.p.), attacked all the cucumber cultivars under open fields conditions, causing a serious damage to foliage and yield of infected plants. The efficiency of some fungicides (Topas, Score, Flent, Thiovit jet and Actamyl) and resistance inducing chemicals (KNO3 and K2HPO4) for management of cucumber powdery mildew disease was evaluated in vitro tests, greenhouse and field conditions. The tested fungicides were significantly inhibited the conidial spore germination of fungus than RICS as well as the control. A significant decrease also was obtained in the disease severity (DS) and the area under disease progress curve (AUDPC) in greenhouse experiment, where the tested fungicides were more effective. Under natural infection, the tested fungicides were highly reduced the DS and AUDPC, than other treatments in field experiments during 2017 and 2018 growing seasons. When the tested fungicides alternated with RICs were more effective and resulted highly increased the fruit yield (quality and quantity) than the tested fungicides and RICS individually in both seasons.

Egyptian Journal of Phytopathology, 2018

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 2020

Plant diseases continue to cause severe damage to most agricultural crops resulting in heavy loss... more Plant diseases continue to cause severe damage to most agricultural crops resulting in heavy losses of both yield and quality. In addition the agricultural wastes cause real problems to get rid of them. Therefore, intensive concern was directed toward compost utilization, as they are available for no cost no side effect. So, in this study different suggested formulations of aerated compost tea in combination with pomegranate peel powder were evaluated for the possibility of using them as an environmentally safe alternative to fungicides. In vitro antifungal activity as well as, the in vivo probability of these combinations to suppress damping-off and wilt diseases caused by R. solani and F. oxysporum was done. Pathogens were isolated from diseased lupine plants with root rot and wilt symptoms. In vitro experiment results illustrated that high inhibition linear growth was obtained by compost/sour pomegranate tea prepared using alkaline water by up to 60% and 70% for F. oxysporum and R. solani respectively. Meanwhile, under field condition all tested combinations showed significant reduction in disease severity over un-composted control, indicated by low wilting and high survival % of lupine plants, no significant differences were detected between treatments. All compost treatments, in the field experiment, performed significantly better than the un-composted control in terms of growth parameters, NPK accumulation, yield and its components with no significant differences. Findings of this study suggest that mixture of pomegranate (Punica granatum L.) peels extract with compost tea might be a promising combination for controlling phytopathogenic fungi and can be used as effective natural fungicide against two of the most dangerous soil-borne fungi looking for reducing the dependence on the chemical fungicides.

Journal of Scientific Research in Science, 2018

Indian Journal of Agricultural Research, 2014

ABSTRACT Inoculation of faba bean seedling with freshly harvested teliospores of two isolates of ... more ABSTRACT Inoculation of faba bean seedling with freshly harvested teliospores of two isolates of Uromyces viciae fabae (Pers.) Schroet, resulted in successful germinating of telispores. Among the 12 faba bean cultivars tested using artificial inoculation, only 4 cultivars developed the five spore stages allowing the completion of the rust life cycle. Pycnia began to appear, as chlorotic flecks only, on the upper surfaces of the leaves 12-14 days after teliospore inoculation. Aecia formed only 10-12 days later on lower surfaces directly opposite to the pycnia formation. Aeciospores were collected and were used for inoculation of faba bean leaves. Uredia were formed 14-16 days later of aeciospores inoculation, under greenhouse conditions

Egyptian Journal of Agricultural Research, 2020

Damping-off and root-rot are important diseases attacking chickpea (Cicer arietinum L.) in Egypt.... more Damping-off and root-rot are important diseases attacking chickpea (Cicer arietinum L.) in Egypt. The effect of biofumigation, using mustard (Brassica juncea) and canola (Brassica napus) seed meals were evaluated to control damping-off and root-rot diseases of chickpea in vitro and in vivo (under greenhouse and field conditions trials) conducted in Sers-Ellian Agricultural Research Station, Menoufia governorate, Egypt, in 2016/2017 and 2017/2018. Both mustard and canola seed meals significantly decreased the linear growth of the tested pathogenic fungi, i.e., Fusarium oxysporum, Sclerotinia sclerotiorum and Rhizoctonia solani, this decrease was more important with the increasing of concentration when compared with the control. The fungicidal effect of mustard and canola seed meals against the tested fungi was demonstrated under greenhouse and field conditions. Obtained results indicated that mustard and canola seed meals significantly reduced the percentages of damping-off severity ...

Journal of Scientific Research in Science

Arab Universities Journal of Agricultural Sciences

Journal of Plant Production

Egyptian Journal of Agricultural Research, 2020

Damping-off and root-rot are important diseases attacking chickpea (Cicer arietinum L.) in Egypt.... more Damping-off and root-rot are important diseases attacking chickpea (Cicer arietinum L.) in Egypt. The effect of biofumigation, using mustard (Brassica juncea) and canola (Brassica napus) seed meals were evaluated to control damping-off and root-rot diseases of chickpea in vitro and in vivo (under greenhouse and field conditions trials) conducted in Sers-Ellian Agricultural Research Station, Menoufia governorate, Egypt, in 2016/2017 and 2017/2018. Both mustard and canola seed meals significantly decreased the linear growth of the tested pathogenic fungi, i.e., Fusarium oxysporum, Sclerotinia sclerotiorum and Rhizoctonia solani, this decrease was more important with the increasing of concentration when compared with the control. The fungicidal effect of mustard and canola seed meals against the tested fungi was demonstrated under greenhouse and field conditions. Obtained results indicated that mustard and canola seed meals significantly reduced the percentages of damping-off severity furthermore improved growth parameters, i.e., plant height, pods number per plant and 100-seed weight. Mustard seed meal recorded the highest values of reduction in this respect as well as, significantly increased nodulation status of rhizobium (Mesorhizobium ciceri) i.e., number of nodules, nodules fresh and dry weights on chickpea roots in pots and field experiments. Mustard seed meal was superior. It could be concluded, that applying of such biofumigation procedure in the control of chickpea damping-off and root rot diseases on the field scale may offer a practical complement environmentally safe measure to control soil-borne pathogens, and may be combined in an integrated diseases management regime.

Egyptian Journal of Biological Pest Control, 2020

Cucumber powdery mildew disease caused by Podosphaera xanthii (Castagne) U. Braun & Shishkoff sev... more Cucumber powdery mildew disease caused by Podosphaera xanthii (Castagne) U. Braun & Shishkoff severe disease-causing yield losses worldwide. This research study was conducted to evaluate the efficacy of the tested bio-agents, Trichoderma harzianum, T. viride, Bacillus subtilis, Paenibacillus polymyxa, and Serratia marcescens, as well as the fungicide score (Difenoconazole), on cucumber infected with P. xanthii, in vitro and under greenhouse conditions. Results indicated that culture filtrate of the tested bio-agents and the fungicide (control) significantly reduced P. xanthii conidial germination in vitro; the reduction percentage ranged between 91.17 and 76.06%. Also, score recorded the highest reduction percentage (97.19%). All treatments significantly decreased the disease severity and area under disease progress curve (AUDPC) post spraying the bio-agents on cucumber plants under greenhouse conditions. Score followed by B. subtilis significantly decreased disease severity percentage (67.33 and 65.38%, respectively) and AUDPC (322.84 and 342.06) than the untreated control (988.13 AUDPC). Additionally, treated cucumber plants showed a significant increase in plant growth parameters (plant height, total chlorophyll, fresh, and dry weight) and yield parameters (fruit number/plant and fruit weight/plant) as well the activity of defense-related enzymes, i.e., peroxidase (PO) and polyphenol oxidase (PPO), and total phenols content (TPC) compared to the untreated plants.

Egyptian Journal of Phytopathology, 2018

Bacillus subtilis, Paenibacillus polymyxa, Pseudomonas fluorescens and Pseudomonas putida isolate... more Bacillus subtilis, Paenibacillus polymyxa, Pseudomonas fluorescens and Pseudomonas putida isolates were evaluated for their biocontrol activities against fodder beet Cercospora leaf spot disease under greenhouse and field conditions compared to the fungicide Topsin M-70. β-1,3-glucanase, peroxidase, polyphenoloxidase and phenylalanine ammonia lyase as well as indolacetic acid and total phenols content were determined in treated and untreated fodder beet plants. Under greenhouse conditions, the reduction in the disease severity of the treated plants with the aforementioned bioagents ranged between 58.82-88.24%. Under field conditions the reduction ranged between 46.67 to 80.00% and 58.33 to 83.33% in the two locations of the experiments i.e., Nubaria and Sakha, respectively. The activities of defense-related enzymes i.e., β-1,3-glucanase, peroxidase, polyphenoloxidase and phenylalanine ammonia lyase were significantly increased in all treated plants with the tested bioagents. P. fluorescens resulted in the highest activity of oxidative enzymes activity. Meanwhile, the contents of indolacetic acid and total phenols were higher in treated plants than the untreated. Also crop parameters i.e., root length, root diameter, fresh and dry weight and % dry matters were significantly increased in the treated fodder beet plants compared to the untreated control. The tested bioagents might be playing an important role in management of Cercospora leaf spot of fodder beet plants through induction of induced systemic resistance.

Arab Universities Journal Agricultural Scince, 2019

The present investigation was carried out to study the effect of seed treatments of lupine plants... more The present investigation was carried out to study the effect of seed treatments of lupine plants (cv.Giza 2) with chemical inducers Bion (5mM), salicylic acid (5mM) and saccharin (3mM) as well as Paenibacillus polymyxa and Trichoderma harzi-anum as biotic inducers on the infection with Rhi-zoctonia solani and Fusarium oxysporum f. sp. lupini under greenhouse and field conditions. Under greenhouse condition (Agricultural Research Centre, Giza) all treatments significantly reduced the percentages of pre-and post-emergence damping-off compared with the untreated control, the highest percentage of survived plants was achieved 92% by Bion and T. harzi-anum as well as fungicide Rizolex-T followed by salicylic acid and P. polymyxa as 88%. On the other hand, all treatments decrease significantly the incidence and severity of wilt, also increased the percentage of survived plants compared with untreated control. Under field conditions at Giza and Ismailia Agricultural Research Stations (Giza and Ismailia governorates) during winter season 2016-2017, all the treatments decreased the percentage of pre-and post-emergence damping-off as well as the percentage of wilted plants and increased the percentage of survived plants compared with untreated control in two locations. At Giza research station , the highest percentages of survived plants were recorded with Rizolex-T followed by Bion, P. polymyxa, and salicylic acid. While at Ismailia, the highest percentage of survived plants were recorded with Rizolex-T followed by Bion, P. poly-myxa, T. harzianum, and salicylic acid. Meantime, these treatments improved growth parameters i.e. plant height, number of pods/plant, the weight of seeds /plant and the weight of one hundred seed. The higher increase in seed yield (ton /feddan) was obtained with Rizolex-T and Bion treatments followed by Salicylic acid, P. polymyxa, and T. har-zianum at two locations. Activities of peroxidase (PO), polyphenol oxi-dase (PPO) enzymes and phenol content were determined. Bion treatment showed the highest increase in PO and PPO activity, and total phenols followed by salicylic acid and P. polymyxa treatments in the presence of R. solani or F. ox-ysporum. f. sp. lupini.

Current Science International, 2019

Powdery mildew disease, Podosphaera xanthii (syn. Sphaerotheca fuliginea auct. p.p.), attacked al... more Powdery mildew disease, Podosphaera xanthii (syn. Sphaerotheca fuliginea auct. p.p.), attacked all the cucumber cultivars under open fields conditions, causing a serious damage to foliage and yield of infected plants. The efficiency of some fungicides (Topas, Score, Flent, Thiovit jet and Actamyl) and resistance inducing chemicals (KNO3 and K2HPO4) for management of cucumber powdery mildew disease was evaluated in vitro tests, greenhouse and field conditions. The tested fungicides were significantly inhibited the conidial spore germination of fungus than RICS as well as the control. A significant decrease also was obtained in the disease severity (DS) and the area under disease progress curve (AUDPC) in greenhouse experiment, where the tested fungicides were more effective. Under natural infection, the tested fungicides were highly reduced the DS and AUDPC, than other treatments in field experiments during 2017 and 2018 growing seasons. When the tested fungicides alternated with RICs were more effective and resulted highly increased the fruit yield (quality and quantity) than the tested fungicides and RICS individually in both seasons.

Journl of Plant Protection and Pathology, 2020

Damping-off and root-rot are some of the most important diseases attack soybean (Glycine max (L.)... more Damping-off and root-rot are some of the most important diseases attack soybean (Glycine max (L.) Merr.) in Egypt. The effect of biocides, i.e. Bio-Cure-F, Plant guard, and Rhizo-N, and essential oils of eucalyptus, thyme, and lemongrass oils, as well as the fungicide Vitavax-200, were evaluated in vitro, greenhouse and under field conditions during the two successive growing seasons, 2018 and 2019 to control damping-off and root-rot diseases of soybean. All the tested biocides and essential oils used significantly inhibited the linear growth of the tested pathogenic fungi, i.e., Fusarium moniliforme, Fusarium solani and Rhizoctonia solani compared with the control. Under greenhouse and field conditions in both growing seasons, obtained results indicated that all the tested biocides and essential oils significantly reduced the percentages of soybean damping-off and root-rot severity furthermore improved photosynthetic pigments content, growth parameters, and seed yield. Vitavax-200 and Plant guard gave the highest values of reduction in this respect, followed by Rhizo-N, lemongrass oil, Bio-Cure-F, thyme oil, and eucalyptus oil respectively. Besides, the quality parameters of soybean seeds showed a favorable elevation in total oil content, on the contrary, there was a general decrease in the seeds protein content. However, the correlations between seeds oil and protein contents were negatively correlated. It could be concluded in biocides and essential oils examined can be recommended for biocontrol of soybean damping-off and root-rot diseases.

The Egyptian Journal of Experimental Biology (Botany), 2020

Plant diseases continue to cause severe damage to most agricultural crops resulting in heavy loss... more Plant diseases continue to cause severe damage to most agricultural crops resulting in heavy losses of both yield and quality. In addition the agricultural wastes cause real problems to get rid of them. Therefore, intensive concern was directed toward compost utilization, as they are available for no cost no side effect. So, in this study different suggested formulations of aerated compost tea in combination with pomegranate peel powder were evaluated for the possibility of using them as an environmentally safe alternative to fungicides. In vitro antifungal activity as well as, the in vivo probability of these combinations to suppress damping-off and wilt diseases caused by R. solani and F. oxysporum was done. Pathogens were isolated from diseased lupine plants with root rot and wilt symptoms. In vitro experiment results illustrated that high inhibition linear growth was obtained by compost/sour pomegranate tea prepared using alkaline water by up to 60% and 70% for F. oxysporum and R. solani respectively. Meanwhile, under field condition all tested combinations showed significant reduction in disease severity over un-composted control, indicated by low wilting and high survival % of lupine plants, no significant differences were detected between treatments. All compost treatments, in the field experiment, performed significantly better than the un-composted control in terms of growth parameters, NPK accumulation, yield and its components with no significant differences. Findings of this study suggest that mixture of pomegranate (Punica granatum L.) peels extract with compost tea might be a promising combination for controlling phytopathogenic fungi and can be used as effective natural fungicide against two of the most dangerous soil-borne fungi looking for reducing the dependence on the chemical fungicides.

Egyptian Journal of Phytopathology, 2018

Journal of Plant Protection and Pathology, 2020

Current Science International, 2019

Powdery mildew disease, Podosphaera xanthii (syn. Sphaerotheca fuliginea auct. p.p.), attacked al... more Powdery mildew disease, Podosphaera xanthii (syn. Sphaerotheca fuliginea auct. p.p.), attacked all the cucumber cultivars under open fields conditions, causing a serious damage to foliage and yield of infected plants. The efficiency of some fungicides (Topas, Score, Flent, Thiovit jet and Actamyl) and resistance inducing chemicals (KNO3 and K2HPO4) for management of cucumber powdery mildew disease was evaluated in vitro tests, greenhouse and field conditions. The tested fungicides were significantly inhibited the conidial spore germination of fungus than RICS as well as the control. A significant decrease also was obtained in the disease severity (DS) and the area under disease progress curve (AUDPC) in greenhouse experiment, where the tested fungicides were more effective. Under natural infection, the tested fungicides were highly reduced the DS and AUDPC, than other treatments in field experiments during 2017 and 2018 growing seasons. When the tested fungicides alternated with RICs were more effective and resulted highly increased the fruit yield (quality and quantity) than the tested fungicides and RICS individually in both seasons.

Egyptian Journal of Phytopathology, 2018

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 2020

Plant diseases continue to cause severe damage to most agricultural crops resulting in heavy loss... more Plant diseases continue to cause severe damage to most agricultural crops resulting in heavy losses of both yield and quality. In addition the agricultural wastes cause real problems to get rid of them. Therefore, intensive concern was directed toward compost utilization, as they are available for no cost no side effect. So, in this study different suggested formulations of aerated compost tea in combination with pomegranate peel powder were evaluated for the possibility of using them as an environmentally safe alternative to fungicides. In vitro antifungal activity as well as, the in vivo probability of these combinations to suppress damping-off and wilt diseases caused by R. solani and F. oxysporum was done. Pathogens were isolated from diseased lupine plants with root rot and wilt symptoms. In vitro experiment results illustrated that high inhibition linear growth was obtained by compost/sour pomegranate tea prepared using alkaline water by up to 60% and 70% for F. oxysporum and R. solani respectively. Meanwhile, under field condition all tested combinations showed significant reduction in disease severity over un-composted control, indicated by low wilting and high survival % of lupine plants, no significant differences were detected between treatments. All compost treatments, in the field experiment, performed significantly better than the un-composted control in terms of growth parameters, NPK accumulation, yield and its components with no significant differences. Findings of this study suggest that mixture of pomegranate (Punica granatum L.) peels extract with compost tea might be a promising combination for controlling phytopathogenic fungi and can be used as effective natural fungicide against two of the most dangerous soil-borne fungi looking for reducing the dependence on the chemical fungicides.

Journal of Scientific Research in Science, 2018

Indian Journal of Agricultural Research, 2014

ABSTRACT Inoculation of faba bean seedling with freshly harvested teliospores of two isolates of ... more ABSTRACT Inoculation of faba bean seedling with freshly harvested teliospores of two isolates of Uromyces viciae fabae (Pers.) Schroet, resulted in successful germinating of telispores. Among the 12 faba bean cultivars tested using artificial inoculation, only 4 cultivars developed the five spore stages allowing the completion of the rust life cycle. Pycnia began to appear, as chlorotic flecks only, on the upper surfaces of the leaves 12-14 days after teliospore inoculation. Aecia formed only 10-12 days later on lower surfaces directly opposite to the pycnia formation. Aeciospores were collected and were used for inoculation of faba bean leaves. Uredia were formed 14-16 days later of aeciospores inoculation, under greenhouse conditions

Egyptian Journal of Agricultural Research, 2020

Damping-off and root-rot are important diseases attacking chickpea (Cicer arietinum L.) in Egypt.... more Damping-off and root-rot are important diseases attacking chickpea (Cicer arietinum L.) in Egypt. The effect of biofumigation, using mustard (Brassica juncea) and canola (Brassica napus) seed meals were evaluated to control damping-off and root-rot diseases of chickpea in vitro and in vivo (under greenhouse and field conditions trials) conducted in Sers-Ellian Agricultural Research Station, Menoufia governorate, Egypt, in 2016/2017 and 2017/2018. Both mustard and canola seed meals significantly decreased the linear growth of the tested pathogenic fungi, i.e., Fusarium oxysporum, Sclerotinia sclerotiorum and Rhizoctonia solani, this decrease was more important with the increasing of concentration when compared with the control. The fungicidal effect of mustard and canola seed meals against the tested fungi was demonstrated under greenhouse and field conditions. Obtained results indicated that mustard and canola seed meals significantly reduced the percentages of damping-off severity ...

Journal of Scientific Research in Science

Arab Universities Journal of Agricultural Sciences

Journal of Plant Production

Egyptian Journal of Agricultural Research, 2020

Damping-off and root-rot are important diseases attacking chickpea (Cicer arietinum L.) in Egypt.... more Damping-off and root-rot are important diseases attacking chickpea (Cicer arietinum L.) in Egypt. The effect of biofumigation, using mustard (Brassica juncea) and canola (Brassica napus) seed meals were evaluated to control damping-off and root-rot diseases of chickpea in vitro and in vivo (under greenhouse and field conditions trials) conducted in Sers-Ellian Agricultural Research Station, Menoufia governorate, Egypt, in 2016/2017 and 2017/2018. Both mustard and canola seed meals significantly decreased the linear growth of the tested pathogenic fungi, i.e., Fusarium oxysporum, Sclerotinia sclerotiorum and Rhizoctonia solani, this decrease was more important with the increasing of concentration when compared with the control. The fungicidal effect of mustard and canola seed meals against the tested fungi was demonstrated under greenhouse and field conditions. Obtained results indicated that mustard and canola seed meals significantly reduced the percentages of damping-off severity furthermore improved growth parameters, i.e., plant height, pods number per plant and 100-seed weight. Mustard seed meal recorded the highest values of reduction in this respect as well as, significantly increased nodulation status of rhizobium (Mesorhizobium ciceri) i.e., number of nodules, nodules fresh and dry weights on chickpea roots in pots and field experiments. Mustard seed meal was superior. It could be concluded, that applying of such biofumigation procedure in the control of chickpea damping-off and root rot diseases on the field scale may offer a practical complement environmentally safe measure to control soil-borne pathogens, and may be combined in an integrated diseases management regime.

Egyptian Journal of Biological Pest Control, 2020

Cucumber powdery mildew disease caused by Podosphaera xanthii (Castagne) U. Braun & Shishkoff sev... more Cucumber powdery mildew disease caused by Podosphaera xanthii (Castagne) U. Braun & Shishkoff severe disease-causing yield losses worldwide. This research study was conducted to evaluate the efficacy of the tested bio-agents, Trichoderma harzianum, T. viride, Bacillus subtilis, Paenibacillus polymyxa, and Serratia marcescens, as well as the fungicide score (Difenoconazole), on cucumber infected with P. xanthii, in vitro and under greenhouse conditions. Results indicated that culture filtrate of the tested bio-agents and the fungicide (control) significantly reduced P. xanthii conidial germination in vitro; the reduction percentage ranged between 91.17 and 76.06%. Also, score recorded the highest reduction percentage (97.19%). All treatments significantly decreased the disease severity and area under disease progress curve (AUDPC) post spraying the bio-agents on cucumber plants under greenhouse conditions. Score followed by B. subtilis significantly decreased disease severity percentage (67.33 and 65.38%, respectively) and AUDPC (322.84 and 342.06) than the untreated control (988.13 AUDPC). Additionally, treated cucumber plants showed a significant increase in plant growth parameters (plant height, total chlorophyll, fresh, and dry weight) and yield parameters (fruit number/plant and fruit weight/plant) as well the activity of defense-related enzymes, i.e., peroxidase (PO) and polyphenol oxidase (PPO), and total phenols content (TPC) compared to the untreated plants.

Egyptian Journal of Phytopathology, 2018

Bacillus subtilis, Paenibacillus polymyxa, Pseudomonas fluorescens and Pseudomonas putida isolate... more Bacillus subtilis, Paenibacillus polymyxa, Pseudomonas fluorescens and Pseudomonas putida isolates were evaluated for their biocontrol activities against fodder beet Cercospora leaf spot disease under greenhouse and field conditions compared to the fungicide Topsin M-70. β-1,3-glucanase, peroxidase, polyphenoloxidase and phenylalanine ammonia lyase as well as indolacetic acid and total phenols content were determined in treated and untreated fodder beet plants. Under greenhouse conditions, the reduction in the disease severity of the treated plants with the aforementioned bioagents ranged between 58.82-88.24%. Under field conditions the reduction ranged between 46.67 to 80.00% and 58.33 to 83.33% in the two locations of the experiments i.e., Nubaria and Sakha, respectively. The activities of defense-related enzymes i.e., β-1,3-glucanase, peroxidase, polyphenoloxidase and phenylalanine ammonia lyase were significantly increased in all treated plants with the tested bioagents. P. fluorescens resulted in the highest activity of oxidative enzymes activity. Meanwhile, the contents of indolacetic acid and total phenols were higher in treated plants than the untreated. Also crop parameters i.e., root length, root diameter, fresh and dry weight and % dry matters were significantly increased in the treated fodder beet plants compared to the untreated control. The tested bioagents might be playing an important role in management of Cercospora leaf spot of fodder beet plants through induction of induced systemic resistance.

Arab Universities Journal Agricultural Scince, 2019

The present investigation was carried out to study the effect of seed treatments of lupine plants... more The present investigation was carried out to study the effect of seed treatments of lupine plants (cv.Giza 2) with chemical inducers Bion (5mM), salicylic acid (5mM) and saccharin (3mM) as well as Paenibacillus polymyxa and Trichoderma harzi-anum as biotic inducers on the infection with Rhi-zoctonia solani and Fusarium oxysporum f. sp. lupini under greenhouse and field conditions. Under greenhouse condition (Agricultural Research Centre, Giza) all treatments significantly reduced the percentages of pre-and post-emergence damping-off compared with the untreated control, the highest percentage of survived plants was achieved 92% by Bion and T. harzi-anum as well as fungicide Rizolex-T followed by salicylic acid and P. polymyxa as 88%. On the other hand, all treatments decrease significantly the incidence and severity of wilt, also increased the percentage of survived plants compared with untreated control. Under field conditions at Giza and Ismailia Agricultural Research Stations (Giza and Ismailia governorates) during winter season 2016-2017, all the treatments decreased the percentage of pre-and post-emergence damping-off as well as the percentage of wilted plants and increased the percentage of survived plants compared with untreated control in two locations. At Giza research station , the highest percentages of survived plants were recorded with Rizolex-T followed by Bion, P. polymyxa, and salicylic acid. While at Ismailia, the highest percentage of survived plants were recorded with Rizolex-T followed by Bion, P. poly-myxa, T. harzianum, and salicylic acid. Meantime, these treatments improved growth parameters i.e. plant height, number of pods/plant, the weight of seeds /plant and the weight of one hundred seed. The higher increase in seed yield (ton /feddan) was obtained with Rizolex-T and Bion treatments followed by Salicylic acid, P. polymyxa, and T. har-zianum at two locations. Activities of peroxidase (PO), polyphenol oxi-dase (PPO) enzymes and phenol content were determined. Bion treatment showed the highest increase in PO and PPO activity, and total phenols followed by salicylic acid and P. polymyxa treatments in the presence of R. solani or F. ox-ysporum. f. sp. lupini.

Current Science International, 2019

Powdery mildew disease, Podosphaera xanthii (syn. Sphaerotheca fuliginea auct. p.p.), attacked al... more Powdery mildew disease, Podosphaera xanthii (syn. Sphaerotheca fuliginea auct. p.p.), attacked all the cucumber cultivars under open fields conditions, causing a serious damage to foliage and yield of infected plants. The efficiency of some fungicides (Topas, Score, Flent, Thiovit jet and Actamyl) and resistance inducing chemicals (KNO3 and K2HPO4) for management of cucumber powdery mildew disease was evaluated in vitro tests, greenhouse and field conditions. The tested fungicides were significantly inhibited the conidial spore germination of fungus than RICS as well as the control. A significant decrease also was obtained in the disease severity (DS) and the area under disease progress curve (AUDPC) in greenhouse experiment, where the tested fungicides were more effective. Under natural infection, the tested fungicides were highly reduced the DS and AUDPC, than other treatments in field experiments during 2017 and 2018 growing seasons. When the tested fungicides alternated with RICs were more effective and resulted highly increased the fruit yield (quality and quantity) than the tested fungicides and RICS individually in both seasons.

Journl of Plant Protection and Pathology, 2020

Damping-off and root-rot are some of the most important diseases attack soybean (Glycine max (L.)... more Damping-off and root-rot are some of the most important diseases attack soybean (Glycine max (L.) Merr.) in Egypt. The effect of biocides, i.e. Bio-Cure-F, Plant guard, and Rhizo-N, and essential oils of eucalyptus, thyme, and lemongrass oils, as well as the fungicide Vitavax-200, were evaluated in vitro, greenhouse and under field conditions during the two successive growing seasons, 2018 and 2019 to control damping-off and root-rot diseases of soybean. All the tested biocides and essential oils used significantly inhibited the linear growth of the tested pathogenic fungi, i.e., Fusarium moniliforme, Fusarium solani and Rhizoctonia solani compared with the control. Under greenhouse and field conditions in both growing seasons, obtained results indicated that all the tested biocides and essential oils significantly reduced the percentages of soybean damping-off and root-rot severity furthermore improved photosynthetic pigments content, growth parameters, and seed yield. Vitavax-200 and Plant guard gave the highest values of reduction in this respect, followed by Rhizo-N, lemongrass oil, Bio-Cure-F, thyme oil, and eucalyptus oil respectively. Besides, the quality parameters of soybean seeds showed a favorable elevation in total oil content, on the contrary, there was a general decrease in the seeds protein content. However, the correlations between seeds oil and protein contents were negatively correlated. It could be concluded in biocides and essential oils examined can be recommended for biocontrol of soybean damping-off and root-rot diseases.

The Egyptian Journal of Experimental Biology (Botany), 2020

Plant diseases continue to cause severe damage to most agricultural crops resulting in heavy loss... more Plant diseases continue to cause severe damage to most agricultural crops resulting in heavy losses of both yield and quality. In addition the agricultural wastes cause real problems to get rid of them. Therefore, intensive concern was directed toward compost utilization, as they are available for no cost no side effect. So, in this study different suggested formulations of aerated compost tea in combination with pomegranate peel powder were evaluated for the possibility of using them as an environmentally safe alternative to fungicides. In vitro antifungal activity as well as, the in vivo probability of these combinations to suppress damping-off and wilt diseases caused by R. solani and F. oxysporum was done. Pathogens were isolated from diseased lupine plants with root rot and wilt symptoms. In vitro experiment results illustrated that high inhibition linear growth was obtained by compost/sour pomegranate tea prepared using alkaline water by up to 60% and 70% for F. oxysporum and R. solani respectively. Meanwhile, under field condition all tested combinations showed significant reduction in disease severity over un-composted control, indicated by low wilting and high survival % of lupine plants, no significant differences were detected between treatments. All compost treatments, in the field experiment, performed significantly better than the un-composted control in terms of growth parameters, NPK accumulation, yield and its components with no significant differences. Findings of this study suggest that mixture of pomegranate (Punica granatum L.) peels extract with compost tea might be a promising combination for controlling phytopathogenic fungi and can be used as effective natural fungicide against two of the most dangerous soil-borne fungi looking for reducing the dependence on the chemical fungicides.