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Papers by pammi gauba

Nanotechnology is expected to open new way by making a very significant impact on the world’s eco... more Nanotechnology is expected to open new way by making a very significant impact on the world’s economy, industry and people’s lives. With the increased globalization of agricultural and food industry, there is a necessity of a developed and innovative tool to restructure the agricultural and food industry through diagnosis and treatment of diseases, enhancing the ability of plants to absorb nutrients and resist microbial and pest infectionsusing the aspects of nanotechnology . Nanoforms of carbon, silver, silica and alumino-silicates are used for controlling plant diseases for example silver is now accepted as an agrochemical replacement that helps in controlling pathogenic micro organisms in the soil. Nanotechnology has provided a solution for protecting the food and agriculture from bacteria, fungi and viral agents. For early detection of plant diseases Nanosensors are used. They are emerging as a promising tool in the agricultural field due to their selectivity, speed and sensitiv...

Microbiology resource announcements, May 26, 2022

Here, we report the genome sequence of PTJIIT1005, isolated from a polluted site on the Yamuna Ri... more Here, we report the genome sequence of PTJIIT1005, isolated from a polluted site on the Yamuna River, Delhi. The genome is complete and consists of ;4.5 Mbp with a GC content of 52.62%, 4,259 protein-coding genes, 76 tRNAs, and 4 rRNAs. Strain PTJIIT1005 shows 98.89% average nucleotide identity (ANI) with Lelliottia amnigena.

International Journal of Current Research and Review, 2021

Introduction: Nitrate is the major concern of groundwater. High concentration arises due to the m... more Introduction: Nitrate is the major concern of groundwater. High concentration arises due to the massive application of chemical fertilizers. It causes various issues Blue Baby Syndrome, respiratory problems etc. Conventional methods are available to remove nitrate, but they can sequester the nitrate and not treat it. Bioremediation is a low-cost technique and is efficient in nitrate removal. Objective: Isolation and identification of nitrate-reducing microbes from the polluted site of Yamuna river. Methods: This paper focuses on microbe's isolationusingBioremediation technique and their identification through Molecular and Biochemical tests. Result: 4 microbes were found to have the potential for remediating nitrate. Microbes were inoculated in nitrate broth and incubated (37⁰C) under both aerobic and anaerobic conditions. Phenotypical and molecular characterization was done. The consensus sequence was submitted in GenBank and accession numbers were obtained.These were Enterobacteraerogenes (MN252552), Lelliotiaamnigena (MN647560), E. coli K12 (MN754025) and Klebsiella oxytoca (MT457847) respectively. After incubation, these strains showed 48.4%, 34.1%, 42.90% and 33.66% of nitrate remediation under anaerobic conditions while almost negligible under aerobic. Further, these microbes were inoculated in broth containing 1500mg/l nitrate equivalent to the maximum reported nitrate in Yamuna River. E. coli and Enterobacter aerogenes remediated 44% and 46% of 1500mg/l nitrate respectively. Conclusion: These two microbes were found to have moderate potential to remediate nitrate (1500mg/l) which brings novelty to this study. A further detailed study on using microbial consortium to enhance the capability of nitrate remediation will be useful.

Journal of Drug Delivery Science and Technology

Materials Today: Proceedings

IOP Conference Series: Earth and Environmental Science, 2021

Hexavalent Chromium is a major soil pollutant; thus, its remediation from soil deserves due atten... more Hexavalent Chromium is a major soil pollutant; thus, its remediation from soil deserves due attention. Phytoremediation is an area of active current research which is eco-friendly and economic. Use of leguminous plants for phytoremediation will improve soil quality, fertility and nutrient balance and would help in restoration of natural soil ecosystem. The present study focuses on the use of two commonly growing legumes; Cicer arietinum (RP1) and Vigna mungo (RP2) to explore their remediation potential towards Cr(VI) with concentration ranging from 100-900 mg kg−1 with the growth up to three weeks and were assessed for remediation potential and toxicity parameters. Higher percentage of decrease in root and shoot length was observed in RP2 as compared to RP1. Chlorophyll content was also found to be decreasing with increasing Cr stress in both the species. RP2 recorded higher BCF than RP1. Highest bioaccumulation factor 4.32 was observed in RP2 at 400 mg kg−1 concentration. Transloca...

BMC Genomics

Lelliottia amnigena PTJIIT1005 is a bacterium that utilizes nitrate as the sole nitrogen source a... more Lelliottia amnigena PTJIIT1005 is a bacterium that utilizes nitrate as the sole nitrogen source and can remediate nitrate from media. The annotation was done related to nitrogen metabolic genes using the PATRIC, RAST tools, and PGAP from the genome sequence of this bacterium. Multiple sequence alignments and phylogenetic analysis of respiratory nitrate reductase, assimilatory nitrate reductase, nitrite reductase, glutamine synthetase, hydroxylamine reductase, nitric oxide reductase genes from PTJIIT1005 were done to find out sequence identities with the most similar species. The identification of operon arrangement in bacteria was also identified. The PATRIC KEGG feature mapped the N-metabolic pathway to identify the chemical process, and the 3D structure of representative enzymes was also elucidated. The putative protein 3D structure was analyzed using I-TASSER software. It gave good quality protein models of all nitrogen metabolism genes and showed good sequence identity with refe...

Frontiers in Nutrition

Probiotics are known as the live microorganisms which upon adequate administration elicit a healt... more Probiotics are known as the live microorganisms which upon adequate administration elicit a health beneficial response inside the host by decreasing the luminal pH, eliminating the pathogenic bacteria in the gut as well as producing short chain fatty acids (SCFA). With advancements in research; probiotics have been explored as potential ingredients in foods. However, their use and applications in food industry have been limited due to restrictions of maintaining the viability of probiotic cells and targeting the successful delivery to gut. Encapsulation techniques have significant influence on increasing the viability rates of probiotic cells with the successful delivery of cells to the target site. Moreover, encapsulating techniques also prevent the live cells from harsh physiological conditions of gut. This review discusses several encapsulating techniques as well as materials derived from natural sources and nutraceutical compounds. In addition to this, this paper also comprehens...

Current Trends in Biotechnology and Pharmacy, 2021

The current study aimed to evaluate the phytotoxicity of tetracycline and amoxicillin on Vigna ra... more The current study aimed to evaluate the phytotoxicity of tetracycline and amoxicillin on Vigna radiata and its remediation potential in hydroponic system. Germinated seeds of Vig-na radiata were planted with varying concentra-tions of tetracycline and amoxicillin (150 – 500 mg L−1) in triplicates (n=3) for three weeks. Tox-icity biomarkers, i.e. changes in plant biomass, photosynthetic pigment, phenol, flavonoid con-tent and antioxidative enzymes were estimated after completion of 3 weeks. The results illus-trated that high tetracycline concentration (500 mg L-1) in hoagland media resulted decrease in total chlorophyll content (3.045- 2.252 mg total chlorophyll /g tissue) while in case of amoxicillin, chlorophyll content was increased (5.18 - 7.309 mg total chlorophyll /g tissue). Carotenoid, Total flavonoid and phenolic content, were also sig-nificantly (p < 0.05) reduced due to toxicity of these antibiotics. Antioxidant enzyme like cata-lase showed 7.22 % degradation in their a...

CABI Reviews

The discovery of antibiotics has led to a major development in providing treatment for different ... more The discovery of antibiotics has led to a major development in providing treatment for different types of bacterial infections. Among the numerous types of antimicrobial agents used, fluoroquinolones have shown potential activity against various harmful pathogens, which cause various kinds of infections pertaining to skin, urinary tract, respiratory tract, gastrointestinal tract as well as sexually transmitted diseases (STDs). Among all classes of fluoroquinolones, ciprofloxacin is the most used and shows broad-spectrum activity against both gram-positive and gram-negative bacteria. The pharmacokinetic profile is higher in comparison with other antibiotics. It inhibits DNA replication and transcription. Although the use of ciprofloxacin has helped in decreasing the death rate, its increased usage has caused escalated contamination in soil and water. This has severely affected humans, plants, animals, and microbes. As ciprofloxacin does not immediately biodegrade, therefore, it can e...

Fermentation

Sea buckthorn is a temperate bush plant native to Asian and European countries, explored across t... more Sea buckthorn is a temperate bush plant native to Asian and European countries, explored across the world in traditional medicine to treat various diseases due to the presence of an exceptionally high content of phenolics, flavonoids and antioxidants. In addition to the evaluation of nutrients and active compounds, the focus of the present work was to assess the optimal levels for L. plantarum RM1 growth by applying response surface methodology (RSM), and to determine the impact of juice fermentation on antioxidant, anti-hypertension and anticancer activity, as well as on organoleptic properties. Sea buckthorn berries were shown to contain good fiber content (6.55%, 25 DV%), high quality of protein (3.12%, 6.24 DV%) containing: histidine, valine, threonine, leucine and lysine (with AAS 24.32, 23.66, 23.09, 23.05 and 21.71%, respectively), and 4.45% sugar that provides only 79 calories. Potassium was shown to be the abundant mineral content (793.43%, 22.66 DV), followed by copper and...

Journal of Scientific and Industrial Research (JSIR), Jun 7, 2021

Over the past decade presence of antibiotics in soil and water is a major environmental concern w... more Over the past decade presence of antibiotics in soil and water is a major environmental concern which needs to be address on a priority basis. The present study was done to evaluate the potential of basil (Ocimum basilicum) for phytoremediation. A greenhouse study was conducted for removal of tetracycline from soil. The plants were grown with 200 mgkg −1 , 400 mgkg −1 , and 600 mgkg −1 of tetracycline for four weeks. Accumulation of tetracycline in shoot and root was observed with HPTLC in plants. They showed a maximum of 97% remediation capability with 200 mgkg −1 of tetracycline treated plants. Secondary metabolites were lepoxygenase pathway products in stress condition. The same were analyzed by GCMS. Alpha-terpineol and methyl acetate completely degraded in all samples, while they were present in plants grown without tetracycline. This could be because antibiotic treatments impact the production of lipoxygenase pathway products, while in some cases secondary metabolites increase marginally as the tetracycline concentrations increased. The aim of the current work was the use of plant-based system for phytoremediation and toxicological impact of tetracycline on basil.

Journal of Life Sciences, 2016

ABSTRACT: Objective: This study focuses on application of phytoremediation technology in remediat... more ABSTRACT: Objective: This study focuses on application of phytoremediation technology in remediation of some pharmaceutical products like antibiotics (ciprofloxacin) and hormones (progesterone and estrogen). Methods: Cicer arientum is well studied and documented plant for phytoremediation of heavy metals, in specific. Here we tried to focus this potential of this plant for the pharmaceutical products by UV-VIS Spectrophotometric method in Hoagland-Arnon solution. Results: The results implies that Cicer arientum can be used for phytoremediation of pharmaceutical products with an average of 60% of the pollutant being remediated. Conclusion: The percentage remediation of pollutants are 60, 64.66 and 63.3 of Ciprofloxacin, progesterone and estrogen, respectively by Cicer arientum. Keywords: Antibiotics, hormones, Cicer arientum , UV spectrophometre , pharmaceutical products.

Indo Global Journal of Pharmaceutical Sciences, 2017

Indo global journal of pharmaceutical sciences, 2014

Phytoremediation is a set of innovative techniques to clean-up the environment taking full advant... more Phytoremediation is a set of innovative techniques to clean-up the environment taking full advantage of the unique metabolic and extractive capabilities of plants. Phytoremediation techniques and processes suggest their applicability to a broad range of contaminants including various organic compounds such as chlorinated solvents, BTEX (Benzene,

Heavy metal toxicity leads to major threats to the environment, leading to adverse effect on plan... more Heavy metal toxicity leads to major threats to the environment, leading to adverse effect on plants and human health. From thousands of years, heavy metals are been used up by the humans. However, threatening health disorders of heavy metals have been known, but still exposure and threats to heavy metal continuous. The increased global population, industrialization and urbanization are some of the major reasons to contaminate the environment. The release of heavy metals occurs from various anthropogenic activities, agronomic practices and dumping of various types of wastes. Heavy metals such as arsenic (As), mercury (Hg), lead (Pb) and cadmium (Cd) are the most toxic heavy metals, according to WHO ranking of the top ten heavy metals. These heavy metals are considered to be intermineable and bio-accumulative in nature, so, do not get readily metabolized, thus remain accumulated in soils and plants. These heavy metals alter the food chain by disturbing the biochemical apparatus of the...

In the present world, where there are high technological advancements, there are serious issue of... more In the present world, where there are high technological advancements, there are serious issue of soil and water pollution with contaminants like heavy metals, antibiotics, hormones, dyes, and petroleum products. Hydroponic experiments were performed to test the ability of Brassica juncea to phytoremediate copper and ciprofloxacin. It was found that B.juncea can remediate higher concentrations (15-30 ppm) of copper more effectively as compared to higher concentrations of ciprofloxacin. In addition to this, it was also observed that lower concentrations (5-10 ppm) of ciprofloxacin can be remediated more effectively than lower concentrations of copper.

Nowadays there is a vast increase in the production and use of antibiotics by humans. Antibiotics... more Nowadays there is a vast increase in the production and use of antibiotics by humans. Antibiotics are also added in animal feed, agriculture, horticulture which has led to a vast amount of release of antibiotics in the environment. This has led to a risk to the environment because antibiotics are continuously added to the soil, ground water, surface water and also they exist as residuals in different environmental compartments. Antibiotics produce antibiotic resistant bacteria which have adverse effects on animal, human health and ecosystem. Due to their antimicrobial nature, biodegradation of antibiotics is difficult and they persist in the environment. The common antibiotics present in environment are ciprofloxacin, tetracycline, sulphonamide, erythromycin, clarithromycin, sulfamethazine, augmentin and many others. The main sources of antibiotic contamination in environment is wastewater released from antibiotic producing industries, hospitals, use of antibiotics by humans, antibi...

Humans have been constantly exploiting nature in the name of development, since the inception of ... more Humans have been constantly exploiting nature in the name of development, since the inception of Earth. Industrial growth, scientific experiments, and destruction of habitats are the leading causes of degradation of ecosystem and deterioration of soil quality. Radioactive matter, industrial effluents, domestic refuse and many other forms of waste is constantly being discharged into the soil and water, and thus jeopardizing the environment. This paper explores the potential of a novel technique, phytoremediation, to resolve the issues of waste management, especially radioactive waste. This technique makes use of green plants to clean up and treat radioactive contaminated sites. Radionuclides such as U-238, Th-232, Ra-226 and Am-241 are extremely dangerous and exposure to them can pose serious health risks. The radiation from these radionuclides can penetrate the cells and tissues of human body and mutate the genetic material, leading to malignant forms of cancers and birth defects. P...

Air pollution is a solemn environmental concern around the whole world. Over last decades increas... more Air pollution is a solemn environmental concern around the whole world. Over last decades increase in industrialization, urbanization and rapid growth in population led to retrogression environmental conditions. Due to challenge of strengthening the economy of country, India is facing issues in protecting its environment as 2014 WHO report India ranked 9 in most polluted countries in the world. According to the WHO report 80% of urban population is exposed to the air quality levels which is exceeded the standard limits. Major polluted cities in India contains high level criteria of pollutants like Sulphur Dioxide(SO2), Nitrogen Dioxide(NO2), Particulate Matter(PM), Greenhouse gases, Ozone precursors and aerosols etc. which deteriorate the environment. In this assessment 3 air pollutants are monitored for air quality evaluation from 7 topographical different cities (Chandigarh, Ahmedabad, Bhopal, Chennai, Bay of Bengal, Shillong, Thiruvendrum) which are SO2, NO2 and PM10.There are va...