SRRM2, CPTAC-1008 - CPTAC Assay Portal (original) (raw)

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SRRM2 KIAA0324, SRL300, SRM300
Sequence Length (AA) Molecular Weight (Da)
2752 299615
Protein Name
Serine/arginine repetitive matrix protein 2
Sources
UniProt PhosphoSitePlus ® GeneCards Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MYNGIGLPTP RGSGTNGYVQ RNLSLVRGRR GERPDYKGEE ELRRLEAALV
60 70 80 90 100
KRPNPDILDH ERKRRVELRC LELEEMMEEQ GYEEQQIQEK VATFRLMLLE
110 120 130 140 150
KDVNPGGKEE TPGQRPAVTE THQLAELNEK KNERLRAAFG ISDSYVDGSS
160 170 180 190 200
FDPQRRAREA KQPAPEPPKP YSLVRESSSS RSPTPKQKKK KKKKDRGRRS
210 220 230 240 250
ESSSPRRERK KSSKKKKHRS ESESKKRKHR SPTPKSKRKS KDKKRKRSRS
260 270 280 290 300
TTPAPKSRRA HRSTSADSAS SSDTSRSRSR SAAAKTHTTA LAGRSPSPAS
310 320 330 340 350
GRRGEGDAPF SEPGTTSTQR PSSPETATKQ PSSPYEDKDK DKKEKSATRP
360 370 380 390 400
SPSPERSSTG PEPPAPTPLL AERHGGSPQP LATTPLSQEP VNPPSEASPT
410 420 430 440 450
RDRSPPKSPE KLPQSSSSES SPPSPQPTKV SRHASSSPES PKPAPAPGSH
460 470 480 490 500
REISSSPTSK NRSHGRAKRD KSHSHTPSRR MGRSRSPATA KRGRSRSRTP
510 520 530 540 550
TKRGHSRSRS PQWRRSRSAQ RWGRSRSPQR RGRSRSPQRP GWSRSRNTQR
560 570 580 590 600
RGRSRSARRG RSHSRSPATR GRSRSRTPAR RGRSRSRTPA RRRSRSRTPT
610 620 630 640 650
RRRSRSRTPA RRGRSRSRTP ARRRSRTRSP VRRRSRSRSP ARRSGRSRSR
660 670 680 690 700
TPARRGRSRS RTPARRGRSR SRTPARRSGR SRSRTPARRG RSRSRTPRRG
710 720 730 740 750
RSRSRSLVRR GRSHSRTPQR RGRSGSSSER KNKSRTSQRR SRSNSSPEMK
760 770 780 790 800
KSRISSRRSR SLSSPRSKAK SRLSLRRSLS GSSPCPKQKS QTPPRRSRSG
810 820 830 840 850
SSQPKAKSRT PPRRSRSSSS PPPKQKSKTP SRQSHSSSSP HPKVKSGTPP
860 870 880 890 900
RQGSITSPQA NEQSVTPQRR SCFESSPDPE LKSRTPSRHS CSGSSPPRVK
910 920 930 940 950
SSTPPRQSPS RSSSPQPKVK AIISPRQRSH SGSSSPSPSR VTSRTTPRRS
960 970 980 990 1000
RSVSPCSNVE SRLLPRYSHS GSSSPDTKVK PETPPRQSHS GSISPYPKVK
1010 1020 1030 1040 1050
AQTPPGPSLS GSKSPCPQEK SKDSLVQSCP GSLSLCAGVK SSTPPGESYF
1060 1070 1080 1090 1100
GVSSLQLKGQ SQTSPDHRSD TSSPEVRQSH SESPSLQSKS QTSPKGGRSR
1110 1120 1130 1140 1150
SSSPVTELAS RSPIRQDRGE FSASPMLKSG MSPEQSRFQS DSSSYPTVDS
1160 1170 1180 1190 1200
NSLLGQSRLE TAESKEKMAL PPQEDATASP PRQKDKFSPF PVQDRPESSL
1210 1220 1230 1240 1250
VFKDTLRTPP RERSGAGSSP ETKEQNSALP TSSQDEELME VVEKSEEPAG
1260 1270 1280 1290 1300
QILSHLSSEL KEMSTSNFES SPEVEERPAV SLTLDQSQSQ ASLEAVEVPS
1310 1320 1330 1340 1350
MASSWGGPHF SPEHKELSNS PLRENSFGSP LEFRNSGPLG TEMNTGFSSE
1360 1370 1380 1390 1400
VKEDLNGPFL NQLETDPSLD MKEQSTRSSG HSSSELSPDA VEKAGMSSNQ
1410 1420 1430 1440 1450
SISSPVLDAV PRTPSRERSS SASSPEMKDG LPRTPSRRSR SGSSPGLRDG
1460 1470 1480 1490 1500
SGTPSRHSLS GSSPGMKDIP RTPSRGRSEC DSSPEPKALP QTPRPRSRSP
1510 1520 1530 1540 1550
SSPELNNKCL TPQRERSGSE SSVDQKTVAR TPLGQRSRSG SSQELDVKPS
1560 1570 1580 1590 1600
ASPQERSESD SSPDSKAKTR TPLRQRSRSG SSPEVDSKSR LSPRRSRSGS
1610 1620 1630 1640 1650
SPEVKDKPRA APRAQSGSDS SPEPKAPAPR ALPRRSRSGS SSKGRGPSPE
1660 1670 1680 1690 1700
GSSSTESSPE HPPKSRTARR GSRSSPEPKT KSRTPPRRRS SRSSPELTRK
1710 1720 1730 1740 1750
ARLSRRSRSA SSSPETRSRT PPRHRRSPSV SSPEPAEKSR SSRRRRSASS
1760 1770 1780 1790 1800
PRTKTTSRRG RSPSPKPRGL QRSRSRSRRE KTRTTRRRDR SGSSQSTSRR
1810 1820 1830 1840 1850
RQRSRSRSRV TRRRRGGSGY HSRSPARQES SRTSSRRRRG RSRTPPTSRK
1860 1870 1880 1890 1900
RSRSRTSPAP WKRSRSRASP ATHRRSRSRT PLISRRRSRS RTSPVSRRRS
1910 1920 1930 1940 1950
RSRTSVTRRR SRSRASPVSR RRSRSRTPPV TRRRSRSRTP TTRRRSRSRT
1960 1970 1980 1990 2000
PPVTRRRSRS RTPPVTRRRS RSRTSPITRR RSRSRTSPVT RRRSRSRTSP
2010 2020 2030 2040 2050
VTRRRSRSRT SPVTRRRSRS RTPPAIRRRS RSRTPLLPRK RSRSRSPLAI
2060 2070 2080 2090 2100
RRRSRSRTPR TARGKRSLTR SPPAIRRRSA SGSSSDRSRS ATPPATRNHS
2110 2120 2130 2140 2150
GSRTPPVALN SSRMSCFSRP SMSPTPLDRC RSPGMLEPLG SSRTPMSVLQ
2160 2170 2180 2190 2200
QAGGSMMDGP GPRIPDHQRT SVPENHAQSR IALALTAISL GTARPPPSMS
2210 2220 2230 2240 2250
AAGLAARMSQ VPAPVPLMSL RTAPAANLAS RIPAASAAAM NLASARTPAI
2260 2270 2280 2290 2300
PTAVNLADSR TPAAAAAMNL ASPRTAVAPS AVNLADPRTP TAPAVNLAGA
2310 2320 2330 2340 2350
RTPAALAALS LTGSGTPPTA ANYPSSSRTP QAPASANLVG PRSAHATAPV
2360 2370 2380 2390 2400
NIAGSRTAAA LAPASLTSAR MAPALSGANL TSPRVPLSAY ERVSGRTSPP
2410 2420 2430 2440 2450
LLDRARSRTP PSAPSQSRMT SERAPSPSSR MGQAPSQSLL PPAQDQPRSP
2460 2470 2480 2490 2500
VPSAFSDQSR CLIAQTTPVA GSQSLSSGAV ATTTSSAGDH NGMLSVPAPG
2510 2520 2530 2540 2550
VPHSDVGEPP ASTGAQQPSA LAALQPAKER RSSSSSSSSS SSSSSSSSSS
2560 2570 2580 2590 2600
SSSSSSGSSS SDSEGSSLPV QPEVALKRVP SPTPAPKEAV REGRPPEPTP
2610 2620 2630 2640 2650
AKRKRRSSSS SSSSSSSSSS SSSSSSSSSS SSSSSSSSSS SSSSSSSSPS
2660 2670 2680 2690 2700
PAKPGPQALP KPASPKKPPP GERRSRSPRK PIDSLRDSRS LSYSPVERRR
2710 2720 2730 2740 2750
PSPQPSPRDQ QSSSSERGSR RGQRGDSRSP SHKRRRETPS PRPMRHRSSR
2752
SP

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1008 Collapse assay details

Data source: Panorama

Official Gene Symbol

SRRM2

Peptide Modified Sequence

ENS[+80.0]FGSPLEFR

Modification Type

Phospho (ST)

Protein - Site of Modification

1326

Peptide - Site of Modification

3

Peptide Start

1324

Peptide End

1334

CPTAC ID

CPTAC-1008

Peptide Molecular Mass

1,361.5653

Species

Homo sapiens (Human)

Assay Type

Enrichment MRM

Enrichment Method

IMAC

Matrix

cell lysate

Submitting Laboratory

Fred Hutchinson Cancer Research Center

Submitting Lab PI

Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

QTrap 5500 (Sciex)

Internal Standard

synthetic peptide

Peptide Standard Purity

Crude

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

nanoLC-2D (Eksigent)

Column Packing

ChromXP C18-CL, 3 µm, 120 Å

Column Dimensions

75 µm x 15cm

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y5 (1+) | 49.3 | 15.9 | 7.2 | 34.6 | 16.4 | 13.9 | 60.2 | 22.8 | 15.7 | 14 | 23 | 15 | | y7 (1+) | 38.7 | 14.4 | 18.2 | 24.3 | 16.2 | 19.2 | 45.7 | 21.7 | 26.5 | 14 | 23 | 15 | | y8 (1+) | 76.1 | 24.9 | 54.1 | 68.6 | 29.4 | 80.4 | 102.5 | 38.5 | 96.9 | 14 | 23 | 15 | | sum | 44.2 | 13.5 | 9.2 | 29.5 | 14.4 | 12 | 53.1 | 19.7 | 15.1 | 14 | 23 | 15 |

Additional Resources and Comments