AURKB, CPTAC-1219 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MAQKENSYPW | PYGRQTAPSG | LSTLPQRVLR | KEPVTPSALV | LMSRSNVQPT |
| 60 | 70 | 80 | 90 | 100 |
| AAPGQKVMEN | SSGTPDILTR | HFTIDDFEIG | RPLGKGKFGN | VYLAREKKSH |
| 110 | 120 | 130 | 140 | 150 |
| FIVALKVLFK | SQIEKEGVEH | QLRREIEIQA | HLHHPNILRL | YNYFYDRRRI |
| 160 | 170 | 180 | 190 | 200 |
| YLILEYAPRG | ELYKELQKSC | TFDEQRTATI | MEELADALMY | CHGKKVIHRD |
| 210 | 220 | 230 | 240 | 250 |
| IKPENLLLGL | KGELKIADFG | WSVHAPSLRR | KTMCGTLDYL | PPEMIEGRMH |
| 260 | 270 | 280 | 290 | 300 |
| NEKVDLWCIG | VLCYELLVGN | PPFESASHNE | TYRRIVKVDL | KFPASVPMGA |
| 310 | 320 | 330 | 340 | 344 |
| QDLISKLLRH | NPSERLPLAQ | VSAHPWVRAN | SRRVLPPSAL | QSVA |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-1340 Collapse assay details
Data source: Panorama
Official Gene Symbol
AURKB
Peptide Modified Sequence
KT[+80.0]MC[+57.0]GTLDYLPPEMIEGR
Modification Type
Phospho (ST), Carbamidomethyl Cysteine
Protein - Site of Modification
232, 234
Peptide - Site of Modification
2, 4
Peptide Start
231
Peptide End
248
CPTAC ID
CPTAC-1340
Peptide Molecular Mass
2,189.9561
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
N/A
Matrix
Digested Cell Lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Quantiva TSQ
Internal Standard
medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 3 um, 200A
Column Dimensions
0.075 x 100 mm
Flow Rate
300 nL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectMRM2
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 17.6 | 6.6 | 4.9 | 17.5 | 8 | 8.4 | 24.8 | 10.4 | 9.7 | 15 | 15 | 15 |
| y7 (1+) | 29.4 | 9.4 | 11.9 | 28.3 | 14.7 | 15.8 | 40.8 | 17.4 | 19.8 | 15 | 15 | 15 |
| y8 (2+) | 4.3 | 5.2 | 3.9 | 5 | 6.2 | 9.7 | 6.6 | 8.1 | 10.5 | 15 | 15 | 15 |
| sum | 6.2 | 4.3 | 3.2 | 7.8 | 5.5 | 8.9 | 10 | 7 | 9.5 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1219 Collapse assay details
Data source: Panorama
Official Gene Symbol
AURKB
Peptide Sequence
LPLAQVSAHPWVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
316
Peptide End
328
CPTAC ID
CPTAC-1219
Peptide Molecular Mass
1,472.8252
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectMRM2
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 31.7 | 7.6 | 7 | 32.2 | 12.3 | 18.9 | 45.2 | 14.5 | 20.2 | 15 | 15 | 15 |
| y7 (1+) | 41.4 | 9.1 | 8 | 51.2 | 20.4 | 15.7 | 65.8 | 22.3 | 17.6 | 15 | 15 | 15 |
| y8 (1+) | 65 | 21.9 | 10.3 | 68.8 | 23.6 | 15.8 | 94.6 | 32.2 | 18.9 | 15 | 15 | 15 |
| sum | 27.3 | 7.2 | 4.9 | 31.1 | 14.8 | 16.3 | 41.4 | 16.5 | 17 | 15 | 15 | 15 |
Selectivity
Data source: Panorama
| Slope of Curve Fit for cell line | ||||||
|---|---|---|---|---|---|---|
| fragment_ion | 1 | 2 | 3 | 4 | 5 | 6 |
| y8 (1+) | 0.03 | 0.03 | 0.04 | 0.05 | 0.06 | 0.06 |
| y7 (1+) | 0.03 | 0.04 | 0.04 | 0.05 | 0.05 | 0.04 |
| y4 (1+) | 0.04 | 0.05 | 0.05 | 0.06 | 0.07 | 0.05 |
| sum | 0.03 | 0.04 | 0.05 | 0.05 | 0.06 | 0.05 |
Stability
Data source: Panorama
| Fragment ion / Transition | control_intra_CV | actual_temp_intra_CV | frozen_intra_CV | FTx1_intra_CV | FTx2_intra_CV |
|---|---|---|---|---|---|
| y4 (1+) | 8.3 | 8.4 | 7.9 | 5 | 12.6 |
| y7 (1+) | 7 | 8.3 | 2.6 | 4.6 | 9.7 |
| y8 (1+) | 12.1 | 8.5 | 9.4 | 12.9 | 10.2 |
| sum | 6.7 | 5.8 | 4.2 | 5.1 | 9.9 |
| Fragment ion / Transition | all_intra_CV | all_inter_CV |
|---|---|---|
| y4 (1+) | 8.4 | 6.9 |
| y7 (1+) | 6.8 | 7.1 |
| y8 (1+) | 10.3 | 9.7 |
| sum | 6.2 | 5.7 |
Endogenous
Data source: Panorama
| Fragment ion / Transition | intra_CV | inter_CV | total_CV |
|---|---|---|---|
| y7 (1+) | 49.5 | 53 | 72.5 |
| y8 (1+) | 72.6 | 89.5 | 115.2 |
| y4 (1+) | 36.5 | 52.8 | 64.2 |
| sum | 32.8 | 48.1 | 58.2 |
Additional Resources and Comments
Assay Details for CPTAC-1228 Collapse assay details
Data source: Panorama
Official Gene Symbol
AURKB
Peptide Sequence
SNVQPTAAPGQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
45
Peptide End
56
CPTAC ID
CPTAC-1228
Peptide Molecular Mass
1,196.6150
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectMRM2
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 34.7 | 11.7 | 17.9 | 49.5 | 14.5 | 18.4 | 60.5 | 18.6 | 25.7 | 15 | 15 | 15 |
| y5 (1+) | 67.3 | 15.6 | 19.7 | 78.2 | 16.1 | 20.6 | 103.2 | 22.4 | 28.5 | 15 | 15 | 15 |
| y8 (1+) | 29.3 | 17.9 | 15.5 | 36.5 | 23.8 | 18 | 46.8 | 29.8 | 23.8 | 15 | 15 | 15 |
| sum | 25.8 | 14.1 | 14.4 | 28.9 | 19.8 | 16.5 | 38.7 | 24.3 | 21.9 | 15 | 15 | 15 |
Selectivity
Data source: Panorama
| Slope of Curve Fit for cell line | ||||||
|---|---|---|---|---|---|---|
| fragment_ion | 1 | 2 | 3 | 4 | 5 | 6 |
| y8 (1+) | 0.16 | 0.17 | 0.15 | 0.15 | 0.15 | 0.15 |
| y5 (1+) | 0.15 | 0.16 | 0.14 | 0.14 | 0.16 | 0.19 |
| y4 (1+) | 0.12 | 0.2 | 0.13 | 0.13 | 0.13 | 0.13 |
| sum | 0.15 | 0.17 | 0.14 | 0.14 | 0.14 | 0.15 |
Stability
Data source: Panorama
| Fragment ion / Transition | control_intra_CV | actual_temp_intra_CV | frozen_intra_CV | FTx1_intra_CV | FTx2_intra_CV |
|---|
| Fragment ion / Transition | all_intra_CV | all_inter_CV |
|---|
Endogenous
Data source: Panorama
| Fragment ion / Transition | intra_CV | inter_CV | total_CV |
|---|---|---|---|
| y8 (1+) | 57.5 | 63.2 | 85.4 |
| y5 (1+) | 70.9 | 85.4 | 111 |
| y4 (1+) | 71.3 | 84.1 | 110.3 |
| sum | 43.7 | 59.8 | 74.1 |
Additional Resources and Comments
Assay Details for CPTAC-3031 Collapse assay details
Data source: Panorama
Official Gene Symbol
AURKB
Peptide Sequence
HFTIDDFEIGRPLGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
71
Peptide End
85
CPTAC ID
CPTAC-3031
Peptide Molecular Mass
1,743.8944
Species
Homo Sapiens
Assay Type
Direct MRM or SRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectMRM2
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 7.9 | 4.4 | 6.4 | 8.3 | 6.6 | 10.5 | 11.5 | 7.9 | 12.3 | 15 | 15 | 15 |
| y7 (1+) | 8.1 | 4.7 | 6.5 | 8.6 | 6.2 | 10.1 | 11.8 | 7.8 | 12 | 15 | 15 | 15 |
| b6 (1+) | 5.4 | 4.6 | 7.1 | 7 | 5.8 | 10 | 8.8 | 7.4 | 12.3 | 15 | 15 | 15 |
| sum | 6.9 | 4.5 | 6.6 | 7.7 | 6 | 10.2 | 10.3 | 7.5 | 12.1 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-3030 Collapse assay details
Data source: Panorama
Official Gene Symbol
AURKB
Peptide Sequence
LPLAQVSAHPWVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
316
Peptide End
328
CPTAC ID
CPTAC-3030
Peptide Molecular Mass
1,472.8252
Species
Homo Sapiens
Assay Type
Direct MRM or SRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectMRM2
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 4.1 | 3.9 | 3.7 | 6.2 | 3.7 | 5.3 | 7.4 | 5.4 | 6.5 | 15 | 15 | 15 |
| y8 (1+) | 3.5 | 4.2 | 4.4 | 7.1 | 4.7 | 6.6 | 7.9 | 6.3 | 7.9 | 15 | 15 | 15 |
| y7 (1+) | 4.1 | 4.4 | 4.5 | 7.9 | 4.4 | 6.5 | 8.9 | 6.2 | 7.9 | 15 | 15 | 15 |
| sum | 3.6 | 4 | 3.9 | 6.6 | 3.7 | 5.6 | 7.5 | 5.4 | 6.8 | 15 | 15 | 15 |