STAT5A, CPTAC-1270 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
CPTAC-1270: View additional ATIISEQQAK data
CPTAC-1271: View additional HILYNEQR data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MAGWIQAQQL | QGDALRQMQV | LYGQHFPIEV | RHYLAQWIES | QPWDAIDLDN |
| 60 | 70 | 80 | 90 | 100 |
| PQDRAQATQL | LEGLVQELQK | KAEHQVGEDG | FLLKIKLGHY | ATQLQKTYDR |
| 110 | 120 | 130 | 140 | 150 |
| CPLELVRCIR | HILYNEQRLV | REANNCSSPA | GILVDAMSQK | HLQINQTFEE |
| 160 | 170 | 180 | 190 | 200 |
| LRLVTQDTEN | ELKKLQQTQE | YFIIQYQESL | RIQAQFAQLA | QLSPQERLSR |
| 210 | 220 | 230 | 240 | 250 |
| ETALQQKQVS | LEAWLQREAQ | TLQQYRVELA | EKHQKTLQLL | RKQQTIILDD |
| 260 | 270 | 280 | 290 | 300 |
| ELIQWKRRQQ | LAGNGGPPEG | SLDVLQSWCE | KLAEIIWQNR | QQIRRAEHLC |
| 310 | 320 | 330 | 340 | 350 |
| QQLPIPGPVE | EMLAEVNATI | TDIISALVTS | TFIIEKQPPQ | VLKTQTKFAA |
| 360 | 370 | 380 | 390 | 400 |
| TVRLLVGGKL | NVHMNPPQVK | ATIISEQQAK | SLLKNENTRN | ECSGEILNNC |
| 410 | 420 | 430 | 440 | 450 |
| CVMEYHQATG | TLSAHFRNMS | LKRIKRADRR | GAESVTEEKF | TVLFESQFSV |
| 460 | 470 | 480 | 490 | 500 |
| GSNELVFQVK | TLSLPVVVIV | HGSQDHNATA | TVLWDNAFAE | PGRVPFAVPD |
| 510 | 520 | 530 | 540 | 550 |
| KVLWPQLCEA | LNMKFKAEVQ | SNRGLTKENL | VFLAQKLFNN | SSSHLEDYSG |
| 560 | 570 | 580 | 590 | 600 |
| LSVSWSQFNR | ENLPGWNYTF | WQWFDGVMEV | LKKHHKPHWN | DGAILGFVNK |
| 610 | 620 | 630 | 640 | 650 |
| QQAHDLLINK | PDGTFLLRFS | DSEIGGITIA | WKFDSPERNL | WNLKPFTTRD |
| 660 | 670 | 680 | 690 | 700 |
| FSIRSLADRL | GDLSYLIYVF | PDRPKDEVFS | KYYTPVLAKA | VDGYVKPQIK |
| 710 | 720 | 730 | 740 | 750 |
| QVVPEFVNAS | ADAGGSSATY | MDQAPSPAVC | PQAPYNMYPQ | NPDHVLDQDG |
| 760 | 770 | 780 | 790 | 794 |
| EFDLDETMDV | ARHVEELLRR | PMDSLDSRLS | PPAGLFTSAR | GSLS |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for non-CPTAC-6252 Collapse assay details
Data source: Panorama
Official Gene Symbol
STAT5A
Peptide Modified Sequence
AVDGY[+79.966331]VKPQIK
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
8
Peptide Start
690
Peptide End
700
CPTAC ID
non-CPTAC-6252
Peptide Molecular Mass
1,296.6479
Species
Human
Assay Type
Enrichment MRM or SRM
Enrichment Method
N/A
Matrix
SKBR3 cells
Submitting Laboratory
Cell Signaling Technology
Submitting Lab PI
Yiying Zhu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Orbitrap Q Exactive (Thermo)
Internal Standard
Heavy isotope-labeled peptides
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
EASY-nLC II (Thermo)
Column Packing
self-packed
Column Dimensions
ID 100um, length 15cm
Flow Rate
300 nL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectMRM2
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 6 | 5.4 | 10.6 | 4.7 | 6.8 | 11.6 | 7.6 | 8.7 | 15.7 | 15 | 15 | 15 |
| y8 (1+) | 6.3 | 5 | 13.4 | 4.6 | 6.9 | 14.1 | 7.8 | 8.5 | 19.5 | 15 | 15 | 15 |
| y9 (1+) | 4.5 | 4.6 | 13.5 | 3.7 | 6.5 | 13.2 | 5.8 | 8 | 18.9 | 15 | 15 | 15 |
| b5 (1+) | 7.9 | 4.3 | 9.7 | 8.6 | 6.1 | 10.8 | 11.7 | 7.5 | 14.5 | 15 | 15 | 15 |
| y9 (2+) | 3.8 | 4.8 | 9.1 | 3.5 | 6.2 | 9.9 | 5.2 | 7.8 | 13.4 | 15 | 15 | 15 |
| y8 (2+) | 5.1 | 4.4 | 10 | 3.4 | 6.2 | 10.4 | 6.1 | 7.6 | 14.4 | 15 | 15 | 15 |
| sum | 4.3 | 4.6 | 10.7 | 3.1 | 6.2 | 11.3 | 5.3 | 7.7 | 15.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1270 Collapse assay details
Data source: Panorama
Official Gene Symbol
STAT5A
Peptide Sequence
ATIISEQQAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
371
Peptide End
380
CPTAC ID
CPTAC-1270
Peptide Molecular Mass
1,087.5873
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectMRM2
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 32 | 12.9 | 10.8 | 52.2 | 14.3 | 13.4 | 61.2 | 19.3 | 17.2 | 15 | 15 | 15 |
| y6 (1+) | 16.1 | 6 | 6.9 | 26.6 | 9.8 | 8.4 | 31.1 | 11.5 | 10.9 | 15 | 15 | 15 |
| y7 (1+) | 16.4 | 6.2 | 8.6 | 22.6 | 10.8 | 6.3 | 27.9 | 12.5 | 10.7 | 15 | 15 | 15 |
| sum | 8.1 | 5.8 | 6.9 | 19.6 | 9.9 | 7 | 21.2 | 11.5 | 9.8 | 15 | 15 | 15 |
Selectivity
Data source: Panorama
| Slope of Curve Fit for cell line | ||||||
|---|---|---|---|---|---|---|
| fragment_ion | 1 | 2 | 3 | 4 | 5 | 6 |
| y6 (1+) | 0.12 | 0.14 | 0.14 | 0.13 | 0.14 | 0.14 |
| y7 (1+) | 0.13 | 0.14 | 0.15 | 0.14 | 0.14 | 0.15 |
| y4 (1+) | 0.13 | 0.11 | 0.14 | 0.14 | 0.12 | 0.13 |
| sum | 0.13 | 0.13 | 0.15 | 0.13 | 0.14 | 0.15 |
Stability
Data source: Panorama
| Fragment ion / Transition | control_intra_CV | actual_temp_intra_CV | frozen_intra_CV | FTx1_intra_CV | FTx2_intra_CV |
|---|---|---|---|---|---|
| y7 (1+) | 3.2 | 8.2 | 2 | 5.3 | 6.3 |
| y6 (1+) | 4.1 | 5.1 | 1.8 | 2.4 | 2.7 |
| y4 (1+) | 9.2 | 10.4 | 22.1 | 3.7 | 14.1 |
| sum | 3.4 | 5.6 | 2 | 2.4 | 4.4 |
| Fragment ion / Transition | all_intra_CV | all_inter_CV |
|---|---|---|
| y7 (1+) | 5.5 | 6.3 |
| y6 (1+) | 3.5 | 4.7 |
| y4 (1+) | 11.6 | 13.4 |
| sum | 3.9 | 4.8 |
Endogenous
Data source: Panorama
| Fragment ion / Transition | intra_CV | inter_CV | total_CV |
|---|---|---|---|
| y4 (1+) | 42.3 | 40.6 | 58.6 |
| y7 (1+) | 19.1 | 37.1 | 41.7 |
| y6 (1+) | 21.3 | 39.9 | 45.2 |
| sum | 19.7 | 38 | 42.8 |
Additional Resources and Comments
Assay Details for CPTAC-1728 Collapse assay details
Data source: Panorama
Official Gene Symbol
STAT5A
Peptide Modified Sequence
AVDGY[+80.0]VKPQIK
Modification Type
Phospho (Y)
Protein - Site of Modification
694
Peptide - Site of Modification
5
Peptide Start
690
Peptide End
700
CPTAC ID
CPTAC-1728
Peptide Molecular Mass
1,296.6479
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM or SRM
Enrichment Method
N/A
Matrix
Ovarian cancer cell lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N V
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectMRM2
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (2+) | 19.1 | 9 | 3.9 | 20.5 | 9.3 | 5.7 | 28 | 12.9 | 6.9 | 15 | 15 | 15 |
| y9 (2+) | 9.5 | 6.6 | 6 | 11.3 | 7.6 | 4.2 | 14.8 | 10.1 | 7.3 | 15 | 15 | 15 |
| y6 (1+) | 17.6 | 5.9 | 7.4 | 26.9 | 5.6 | 9 | 32.1 | 8.1 | 11.7 | 15 | 15 | 15 |
| sum | 8.9 | 4.3 | 3.4 | 10.3 | 5.3 | 3.1 | 13.6 | 6.8 | 4.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1271 Collapse assay details
Data source: Panorama
Official Gene Symbol
STAT5A
Peptide Sequence
HILYNEQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
111
Peptide End
118
CPTAC ID
CPTAC-1271
Peptide Molecular Mass
1,071.5461
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectMRM2
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 27.8 | 9.5 | 19.9 | 34.1 | 14.6 | 19.1 | 44 | 17.4 | 27.6 | 15 | 15 | 15 |
| y6 (1+) | 7.2 | 4.6 | 6.5 | 13.5 | 7 | 8.4 | 15.3 | 8.4 | 10.6 | 15 | 15 | 15 |
| y7 (1+) | 14 | 6.7 | 6 | 19.1 | 8.5 | 6.5 | 23.7 | 10.8 | 8.8 | 15 | 15 | 15 |
| sum | 7.9 | 4.2 | 6.4 | 13.6 | 7 | 7.8 | 15.7 | 8.2 | 10.1 | 15 | 15 | 15 |
Selectivity
Data source: Panorama
| Slope of Curve Fit for cell line | ||||||
|---|---|---|---|---|---|---|
| fragment_ion | 1 | 2 | 3 | 4 | 5 | 6 |
| y7 (1+) | 2.23 | 2.2 | 2.14 | 2.09 | 2.15 | 2.16 |
| y6 (1+) | 2.03 | 2.04 | 2.1 | 2.24 | 2.25 | 2.24 |
| y4 (1+) | 1.31 | 1.95 | 1.38 | 1.93 | 1.27 | 1.34 |
| sum | 2.02 | 2.06 | 2.07 | 2.19 | 2.19 | 2.16 |
Stability
Data source: Panorama
| Fragment ion / Transition | control_intra_CV | actual_temp_intra_CV | frozen_intra_CV | FTx1_intra_CV | FTx2_intra_CV |
|---|
| Fragment ion / Transition | all_intra_CV | all_inter_CV |
|---|
Endogenous
Data source: Panorama
| Fragment ion / Transition | intra_CV | inter_CV | total_CV |
|---|---|---|---|
| y4 (1+) | 64.1 | 86.8 | 107.9 |
| y6 (1+) | 26.7 | 41.3 | 49.2 |
| y7 (1+) | 37.2 | 40.8 | 55.2 |
| sum | 27.2 | 40.8 | 49 |