STAT2, CPTAC-1273 - CPTAC Assay Portal (original) (raw)

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

STAT2

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAQWEMLQNL DSPFQDQLHQ LYSHSLLPVD IRQYLAVWIE DQNWQEAALG
60 70 80 90 100
SDDSKATMLF FHFLDQLNYE CGRCSQDPES LLLQHNLRKF CRDIQPFSQD
110 120 130 140 150
PTQLAEMIFN LLLEEKRILI QAQRAQLEQG EPVLETPVES QQHEIESRIL
160 170 180 190 200
DLRAMMEKLV KSISQLKDQQ DVFCFRYKIQ AKGKTPSLDP HQTKEQKILQ
210 220 230 240 250
ETLNELDKRR KEVLDASKAL LGRLTTLIEL LLPKLEEWKA QQQKACIRAP
260 270 280 290 300
IDHGLEQLET WFTAGAKLLF HLRQLLKELK GLSCLVSYQD DPLTKGVDLR
310 320 330 340 350
NAQVTELLQR LLHRAFVVET QPCMPQTPHR PLILKTGSKF TVRTRLLVRL
360 370 380 390 400
QEGNESLTVE VSIDRNPPQL QGFRKFNILT SNQKTLTPEK GQSQGLIWDF
410 420 430 440 450
GYLTLVEQRS GGSGKGSNKG PLGVTEELHI ISFTVKYTYQ GLKQELKTDT
460 470 480 490 500
LPVVIISNMN QLSIAWASVL WFNLLSPNLQ NQQFFSNPPK APWSLLGPAL
510 520 530 540 550
SWQFSSYVGR GLNSDQLSML RNKLFGQNCR TEDPLLSWAD FTKRESPPGK
560 570 580 590 600
LPFWTWLDKI LELVHDHLKD LWNDGRIMGF VSRSQERRLL KKTMSGTFLL
610 620 630 640 650
RFSESSEGGI TCSWVEHQDD DKVLIYSVQP YTKEVLQSLP LTEIIRHYQL
660 670 680 690 700
LTEENIPENP LRFLYPRIPR DEAFGCYYQE KVNLQERRKY LKHRLIVVSN
710 720 730 740 750
RQVDELQQPL ELKPEPELES LELELGLVPE PELSLDLEPL LKAGLDLGPE
760 770 780 790 800
LESVLESTLE PVIEPTLCMV SQTVPEPDQG PVSQPVPEPD LPCDLRHLNT
810 820 830 840 850
EPMEIFRNCV KIEEIMPNGD PLLAGQNTVD EVYVSRPSHF YTDGPLMPSD
851
F

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1273 Collapse assay details

Data source: Panorama

Official Gene Symbol

STAT2

Peptide Sequence

EVLQSLPLTEIIR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

634

Peptide End

646

CPTAC ID

CPTAC-1273

Peptide Molecular Mass

1,509.8766

Species

Homo sapiens (Human)

Assay Type

Direct MRM or SRM

Matrix

Ovarian cancer tumor tissue lysate

Submitting Laboratory

Pacific Northwest National Laboratory

Submitting Lab PI

Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

TSQ Vantage

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

Waters nanoACQUITY (Part Number 176016000)

Column Packing

Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)

Column Dimensions

100 um x 100 mm

Flow Rate

0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y7 (1+) | 9.8 | 16.5 | 20.4 | 32 | 14.5 | 7.9 | 33.5 | 22 | 21.9 | 15 | 15 | 15 | | y8 (1+) | 19.8 | 15.3 | 24.9 | 23.4 | 29.5 | 19.4 | 30.7 | 33.2 | 31.6 | 15 | 15 | 15 | | y9 (1+) | 13.8 | 17.3 | 21.2 | 31.2 | 18.2 | 12.9 | 34.1 | 25.1 | 24.8 | 15 | 15 | 15 | | sum | 11.2 | 15.1 | 20 | 30.6 | 16.6 | 9.4 | 32.6 | 22.4 | 22.1 | 15 | 15 | 15 |

Additional Resources and Comments

Comments

Assay Details for CPTAC-1274 Collapse assay details

Data source: Panorama

Official Gene Symbol

STAT2

Peptide Sequence

HYQLLTEENIPENPLR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

647

Peptide End

662

CPTAC ID

CPTAC-1274

Peptide Molecular Mass

1,964.9956

Species

Homo sapiens (Human)

Assay Type

Direct MRM or SRM

Matrix

Ovarian cancer tumor tissue lysate

Submitting Laboratory

Pacific Northwest National Laboratory

Submitting Lab PI

Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

TSQ Vantage

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

Waters nanoACQUITY (Part Number 176016000)

Column Packing

Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)

Column Dimensions

100 um x 100 mm

Flow Rate

0.5 uL/min

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-DirectMRM2

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y4 (1+) | 33.1 | 30.4 | 35.5 | 53.6 | 40.7 | 27.8 | 63 | 50.8 | 45.1 | 15 | 15 | 15 | | y6 (1+) | 8.9 | 16 | 17.7 | 34.4 | 20.6 | 12.7 | 35.5 | 26.1 | 21.8 | 15 | 15 | 15 | | y8 (1+) | 29.2 | 26.3 | 25.3 | 41.1 | 32.7 | 26.1 | 50.4 | 42 | 36.3 | 15 | 15 | 15 | | sum | 8.9 | 12.3 | 18.6 | 32.8 | 18.4 | 11.1 | 34 | 22.1 | 21.7 | 15 | 15 | 15 |

Additional Resources and Comments

Comments