NPY, CPTAC-1287 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
CPTAC-1290: View additional HYINLITR data
non-CPTAC-3487: View additional HYINLITR data
non-CPTAC-3487: View additional HYINLITR data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MLGNKRLGLS | GLTLALSLLV | CLGALAEAYP | SKPDNPGEDA | PAEDMARYYS |
| 60 | 70 | 80 | 90 | 97 |
| ALRHYINLIT | RQRYGKRSSP | ETLISDLLMR | ESTENVPRTR | LEDPAMW |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-1290 Collapse assay details
Data source: Panorama
Official Gene Symbol
NPY
Peptide Sequence
HYINLITR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
54
Peptide End
61
CPTAC ID
CPTAC-1290
Peptide Molecular Mass
1,028.5767
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min
Assay Multiplexing Expand assay panel
Johns Hopkins University-C
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 50 | 12.8 | 16.2 | 68 | 13.7 | 18.4 | 84.4 | 18.7 | 24.5 | 15 | 15 | 15 |
| y4 (1+) | 46.3 | 12.3 | 14.6 | 49 | 14 | 15.9 | 67.4 | 18.6 | 21.6 | 15 | 15 | 15 |
| y6 (1+) | 12.5 | 3.2 | 2.3 | 16.1 | 3.6 | 5.3 | 20.4 | 4.8 | 5.8 | 15 | 15 | 15 |
| sum | 10.9 | 3 | 1.9 | 15.6 | 3.6 | 4.8 | 19 | 4.7 | 5.2 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1288 Collapse assay details
Data source: Panorama
Official Gene Symbol
NPY
Peptide Sequence
SKPDNPGEDAPAEDMAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
31
Peptide End
47
CPTAC ID
CPTAC-1288
Peptide Molecular Mass
1,798.7792
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min
Assay Multiplexing Expand assay panel
Johns Hopkins University-C
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 29.1 | 13.9 | 13.1 | 25.7 | 12.4 | 12.5 | 38.8 | 18.6 | 18.1 | 15 | 15 | 15 |
| y6 (1+) | 21.1 | 8.5 | 8.9 | 23.1 | 8.6 | 10.6 | 31.3 | 12.1 | 13.8 | 15 | 15 | 15 |
| y7 (1+) | 17.3 | 5.3 | 6 | 24.9 | 5.7 | 5.5 | 30.3 | 7.8 | 8.1 | 15 | 15 | 15 |
| sum | 16 | 3.7 | 5.7 | 21.8 | 3.8 | 4.9 | 27 | 5.3 | 7.5 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1289 Collapse assay details
Data source: Panorama
Official Gene Symbol
NPY
Peptide Modified Sequence
SKPDNPGEDAPAEDM[+16.0]AR
Modification Type
Oxidation (M)
Protein - Site of Modification
45
Peptide - Site of Modification
15
Peptide Start
31
Peptide End
47
CPTAC ID
CPTAC-1289
Peptide Molecular Mass
1,814.7741
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min
Assay Multiplexing Expand assay panel
Johns Hopkins University-C
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 24.3 | 7.7 | 7.2 | 35.6 | 7 | 7.9 | 43.1 | 10.4 | 10.7 | 15 | 15 | 15 |
| y6 (1+) | 13.3 | 7.8 | 8.6 | 17.3 | 10.8 | 11.7 | 21.8 | 13.3 | 14.5 | 15 | 15 | 15 |
| y7 (1+) | 19.3 | 7.8 | 7.6 | 24.6 | 7.1 | 7.5 | 31.3 | 10.5 | 10.7 | 15 | 15 | 15 |
| sum | 15.8 | 3.7 | 5.8 | 21.7 | 5.6 | 6 | 26.8 | 6.7 | 8.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1291 Collapse assay details
Data source: Panorama
Official Gene Symbol
NPY
Peptide Sequence
SSPETLISDLLMR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
68
Peptide End
80
CPTAC ID
CPTAC-1291
Peptide Molecular Mass
1,460.7545
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min
Assay Multiplexing Expand assay panel
Johns Hopkins University-C
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 49.8 | 7.9 | 4.8 | 44.6 | 6.8 | 4.6 | 66.9 | 10.4 | 6.6 | 15 | 15 | 15 |
| y7 (1+) | 46.2 | 8 | 7.8 | 50.1 | 8.3 | 7.8 | 68.2 | 11.5 | 11 | 15 | 15 | 15 |
| y9 (1+) | 65 | 25.8 | 21.5 | 87.4 | 23.7 | 22.9 | 108.9 | 35 | 31.4 | 15 | 15 | 15 |
| sum | 37.5 | 5.2 | 5.2 | 33.9 | 5.2 | 4.9 | 50.6 | 7.4 | 7.1 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1287 Collapse assay details
Data source: Panorama
Official Gene Symbol
NPY
Peptide Sequence
YPSKPDNPGEDAPAEDMAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
29
Peptide End
47
CPTAC ID
CPTAC-1287
Peptide Molecular Mass
2,058.8953
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min
Assay Multiplexing Expand assay panel
Johns Hopkins University-C
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (2+) | 13.8 | 7.8 | 6.7 | 19.5 | 9 | 8 | 23.9 | 11.9 | 10.4 | 15 | 15 | 15 |
| y6 (1+) | 77.7 | 21 | 16.8 | 91 | 20.1 | 16.5 | 119.7 | 29.1 | 23.5 | 15 | 15 | 15 |
| y7 (1+) | 16.9 | 7.4 | 8.4 | 18.3 | 7.7 | 8 | 24.9 | 10.7 | 11.6 | 15 | 15 | 15 |
| sum | 14.5 | 5.7 | 4.2 | 19.8 | 5.6 | 4.3 | 24.5 | 8 | 6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-3487 Collapse assay details
Data source: Panorama
Official Gene Symbol
Npy
Peptide Sequence
HYINLITR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
54
Peptide End
61
CPTAC ID
non-CPTAC-3487
Peptide Molecular Mass
1,028.5767
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Assay Multiplexing Expand assay panel
Johns Hopkins University-C
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b2 (1+) | 4.1 | 4.5 | 2.7 | 6.2 | 6.1 | 6.7 | 7.4 | 7.6 | 7.2 | 15 | 15 | 15 |
| y3 (2+) | 47.2 | 39.9 | 68.1 | 54.4 | 39.4 | 84.3 | 72 | 56.1 | 108.4 | 15 | 15 | 15 |
| y5 (1+) | 6.9 | 3 | 5.1 | 9 | 6.9 | 5.3 | 11.3 | 7.5 | 7.4 | 15 | 15 | 15 |
| y6 (1+) | 5.2 | 3.1 | 2.9 | 9.5 | 6.1 | 5.5 | 10.8 | 6.8 | 6.2 | 15 | 15 | 15 |
| y7 (1+) | 7.1 | 3.9 | 4.2 | 12.9 | 9.5 | 10 | 14.7 | 10.3 | 10.8 | 15 | 15 | 15 |
| sum | 3.4 | 2.1 | 1.7 | 8 | 6.6 | 4.7 | 8.7 | 6.9 | 5 | 15 | 15 | 15 |