KLK3, CPTAC-1296 - CPTAC Assay Portal (original) (raw)
Official Gene Symbol
KLK3
Peptide Modified Sequence
IVGGWEC[+57.0]EK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
31
Peptide - Site of Modification
7
Peptide Start
25
Peptide End
33
CPTAC ID
CPTAC-1476
Peptide Molecular Mass
1,076.4961
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr
Publication
View Details (opens in a new window)
Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 7.4 | 2.2 | 2.5 | 9.3 | 5.5 | 3.7 | 11.9 | 5.9 | 4.5 | 15 | 15 | 15 |
| y7 (1+) | 3.3 | 1.7 | 1.2 | 3.2 | 5.8 | 4.2 | 4.6 | 6 | 4.4 | 15 | 15 | 15 |
| y3 (1+) | 3.8 | 2.9 | 1.9 | 5.1 | 5.9 | 4.8 | 6.4 | 6.6 | 5.2 | 15 | 15 | 15 |
| sum | 2.7 | 1.8 | 1.3 | 3.2 | 5.7 | 4.2 | 4.2 | 6 | 4.4 | 15 | 15 | 15 |