MUC1, CPTAC-146 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MTPGTQSPFF | LLLLLTVLTV | VTGSGHASST | PGGEKETSAT | QRSSVPSSTE |
| 60 | 70 | 80 | 90 | 100 |
| KNAVSMTSSV | LSSHSPGSGS | STTQGQDVTL | APATEPASGS | AATWGQDVTS |
| 110 | 120 | 130 | 140 | 150 |
| VPVTRPALGS | TTPPAHDVTS | APDNKPAPGS | TAPPAHGVTS | APDTRPAPGS |
| 160 | 170 | 180 | 190 | 200 |
| TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS |
| 210 | 220 | 230 | 240 | 250 |
| APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS |
| 260 | 270 | 280 | 290 | 300 |
| TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS |
| 310 | 320 | 330 | 340 | 350 |
| APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS |
| 360 | 370 | 380 | 390 | 400 |
| TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS |
| 410 | 420 | 430 | 440 | 450 |
| APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS |
| 460 | 470 | 480 | 490 | 500 |
| TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS |
| 510 | 520 | 530 | 540 | 550 |
| APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS |
| 560 | 570 | 580 | 590 | 600 |
| TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS |
| 610 | 620 | 630 | 640 | 650 |
| APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS |
| 660 | 670 | 680 | 690 | 700 |
| TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS |
| 710 | 720 | 730 | 740 | 750 |
| APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS |
| 760 | 770 | 780 | 790 | 800 |
| TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS |
| 810 | 820 | 830 | 840 | 850 |
| APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS |
| 860 | 870 | 880 | 890 | 900 |
| TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS |
| 910 | 920 | 930 | 940 | 950 |
| APDTRPAPGS | TAPPAHGVTS | APDTRPAPGS | TAPPAHGVTS | APDNRPALGS |
| 960 | 970 | 980 | 990 | 1000 |
| TAPPVHNVTS | ASGSASGSAS | TLVHNGTSAR | ATTTPASKST | PFSIPSHHSD |
| 1010 | 1020 | 1030 | 1040 | 1050 |
| TPTTLASHST | KTDASSTHHS | SVPPLTSSNH | STSPQLSTGV | SFFFLSFHIS |
| 1060 | 1070 | 1080 | 1090 | 1100 |
| NLQFNSSLED | PSTDYYQELQ | RDISEMFLQI | YKQGGFLGLS | NIKFRPGSVV |
| 1110 | 1120 | 1130 | 1140 | 1150 |
| VQLTLAFREG | TINVHDVETQ | FNQYKTEAAS | RYNLTISDVS | VSDVPFPFSA |
| 1160 | 1170 | 1180 | 1190 | 1200 |
| QSGAGVPGWG | IALLVLVCVL | VALAIVYLIA | LAVCQCRRKN | YGQLDIFPAR |
| 1210 | 1220 | 1230 | 1240 | 1250 |
| DTYHPMSEYP | TYHTHGRYVP | PSSTDRSPYE | KVSAGNGGSS | LSYTNPAVAA |
| 1255 | ||||
| TSANL |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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to view detailed assay information below
All other points link out to UniProt
Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-146 Collapse assay details
Data source: Panorama
Official Gene Symbol
MUC1
Peptide Sequence
EGTINVHDVETQFNQYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1109
Peptide End
1125
CPTAC ID
CPTAC-146
Peptide Molecular Mass
2,020.9490
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 11.3 | 9.7 | 7.3 | 12.3 | 11.1 | 12 | 16.7 | 14.7 | 14 | 25 | 15 | 15 |
| y6 (1+) | 10.1 | 14.2 | 7.9 | 16 | 22.6 | 33.8 | 18.9 | 26.7 | 34.7 | 25 | 15 | 15 |
| y5 (1+) | 5.9 | 5.5 | 6.6 | 11.6 | 10.3 | 10.1 | 13 | 11.7 | 12.1 | 25 | 15 | 15 |
| sum | 5.9 | 5 | 5.8 | 10.1 | 8.6 | 12.6 | 11.7 | 9.9 | 13.9 | 25 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-719 Collapse assay details
Data source: Panorama
Official Gene Symbol
MUC1
Peptide Sequence
EGTINVHDVETQFNQYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1109
Peptide End
1125
CPTAC ID
CPTAC-719
Peptide Molecular Mass
2,020.9490
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 19 | 5.9 | 6 | 26.8 | 8.9 | 7.2 | 32.9 | 10.7 | 9.4 | 15 | 15 | 15 |
| y6 (1+) | 17 | 5.6 | 3.8 | 20.4 | 8.2 | 8 | 26.6 | 9.9 | 8.9 | 15 | 15 | 15 |
| y7 (1+) | 18.7 | 3.8 | 4 | 26.4 | 8.5 | 6.5 | 32.4 | 9.3 | 7.6 | 15 | 15 | 15 |
| sum | 15.3 | 3.5 | 3.6 | 23.8 | 7.6 | 5.5 | 28.3 | 8.4 | 6.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-147 Collapse assay details
Data source: Panorama
Official Gene Symbol
MUC1
Peptide Sequence
NYGQLDIFPAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1190
Peptide End
1200
CPTAC ID
CPTAC-147
Peptide Molecular Mass
1,292.6513
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 12.8 | 7.5 | 7.2 | 22.6 | 11.3 | 6.6 | 26 | 13.6 | 9.8 | 15 | 25 | 15 |
| y7 (1+) | 17 | 7 | 7.3 | 19 | 10.3 | 10.3 | 25.5 | 12.5 | 12.6 | 15 | 25 | 15 |
| y6 (1+) | 9.9 | 8.2 | 3.7 | 19 | 13.7 | 24 | 21.4 | 16 | 24.3 | 15 | 25 | 15 |
| sum | 8.9 | 6.1 | 3.4 | 15.4 | 10.5 | 12.6 | 17.8 | 12.1 | 13.1 | 15 | 25 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-730 Collapse assay details
Data source: Panorama
Official Gene Symbol
MUC1
Peptide Sequence
NYGQLDIFPAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1190
Peptide End
1200
CPTAC ID
CPTAC-730
Peptide Molecular Mass
1,292.6513
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b4 (1+) | 57.1 | 15.1 | 4.5 | 53.6 | 15.9 | 8.5 | 78.3 | 21.9 | 9.6 | 15 | 15 | 15 |
| b3 (1+) | 36 | 7.5 | 3 | 27.6 | 10.2 | 8.7 | 45.4 | 12.7 | 9.2 | 15 | 15 | 15 |
| y4 (1+) | 29.9 | 5.8 | 4.4 | 29.4 | 7.8 | 9 | 41.9 | 9.7 | 10 | 15 | 15 | 15 |
| sum | 30.5 | 6.1 | 3.3 | 23.7 | 7.7 | 8.5 | 38.6 | 9.8 | 9.1 | 15 | 15 | 15 |