MUC2, CPTAC-1497 - CPTAC Assay Portal (original) (raw)

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1497 Collapse assay details

Data source: Panorama

Official Gene Symbol

MUC2

Peptide Modified Sequence

GDHN[+1.0]DSYALLGELAPC[+57.0]GSTDK

Modification Type

Deamidated (N), Carbamidomethyl (C)

Protein - Site of Modification

423, 435

Peptide - Site of Modification

4, 16

Peptide Start

420

Peptide End

440

CPTAC ID

CPTAC-1497

Peptide Molecular Mass

2,219.9641

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

Human ovarian tumor tissue digest

Submitting Laboratory

Johns Hopkins University

Submitting Lab PI

Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

TSQ Vantage

Internal Standard

Stable isotope-labeled peptides

Peptide Standard Purity

Crude (~60%)

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

Accela 1250 Quaternary Low Pump

Column Packing

C18, 3 µm, 300 Å

Column Dimensions

1.0 mm I.D. x 15 cm

Flow Rate

50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-E

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y7 (1+) | 3.4 | 2.6 | 4.1 | 4.3 | 3.1 | 3.8 | 5.5 | 4 | 5.6 | 15 | 15 | 15 | | y8 (1+) | 6.8 | 3.5 | 6.7 | 7.3 | 4.1 | 7.1 | 10 | 5.4 | 9.8 | 15 | 15 | 15 | | b9 (1+) | 6.2 | 5.2 | 3.8 | 5.5 | 5.7 | 4.4 | 8.3 | 7.7 | 5.8 | 15 | 15 | 15 | | sum | 2.7 | 1.3 | 2.2 | 3.4 | 1.5 | 2.7 | 4.3 | 2 | 3.5 | 15 | 15 | 15 |

Additional Resources and Comments