KRAS, CPTAC-1550 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Assay Details for non-CPTAC-6104 Collapse assay details
Data source: Panorama
Official Gene Symbol
KRAS
Peptide Sequence
LVVVGAVGVGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1
Peptide End
11
CPTAC ID
non-CPTAC-6104
Peptide Molecular Mass
996.6332
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Bovine Serum Albumin
Submitting Laboratory
McGill University
Submitting Lab PI
Christoph H. Borchers, Gerald Batist
Publication
View Details (opens in a new window)
mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Agilent 6495B QQQ-MS
Internal Standard
Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Infinity 1290
Column Packing
commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions
4.6 mm × 150 mm, (5 µm)
Flow Rate
0.4 mL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectSRM2
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b2 (1+) | 16.7 | 6.6 | 4.2 | 21.1 | 6.7 | 5.2 | 26.9 | 9.4 | 6.7 | 15 | 16 | 17 |
| b3 (1+) | 16.6 | 4.8 | 8.4 | 21 | 7.2 | 7.3 | 26.8 | 8.7 | 11.1 | 15 | 16 | 17 |
| y7 (1+) | 19 | 3.9 | 6.1 | 23.2 | 2.7 | 5.9 | 30 | 4.7 | 8.5 | 15 | 16 | 17 |
| y8 (1+) | 16.1 | 7 | 9.2 | 18 | 8.3 | 7.4 | 24.1 | 10.9 | 11.8 | 15 | 16 | 17 |
| y9 (1+) | 18.5 | 7.2 | 7.9 | 21.5 | 6.6 | 9.6 | 28.4 | 9.8 | 12.4 | 15 | 16 | 17 |
| sum | 14.9 | 1.9 | 2.4 | 17.4 | 1.7 | 2.6 | 22.9 | 2.5 | 3.5 | 15 | 16 | 17 |