HRAS, CPTAC-1551 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
HRAS
Protein Sequence hover to view complete sequence
CPTAC-1551: View additional LVVVGAGGVGK data
CPTAC-1552: View additional SFEDIHQYR data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MTEYKLVVVG | AGGVGKSALT | IQLIQNHFVD | EYDPTIEDSY | RKQVVIDGET |
| 60 | 70 | 80 | 90 | 100 |
| CLLDILDTAG | QEEYSAMRDQ | YMRTGEGFLC | VFAINNTKSF | EDIHQYREQI |
| 110 | 120 | 130 | 140 | 150 |
| KRVKDSDDVP | MVLVGNKCDL | AARTVESRQA | QDLARSYGIP | YIETSAKTRQ |
| 160 | 170 | 180 | 189 | |
| GVEDAFYTLV | REIRQHKLRK | LNPPDESGPG | CMSCKCVLS |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-1551 Collapse assay details
Data source: Panorama
Official Gene Symbol
HRAS
Peptide Sequence
LVVVGAGGVGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
6
Peptide End
16
CPTAC ID
CPTAC-1551
Peptide Molecular Mass
954.5862
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectSRM2
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 6.5 | 3.3 | 2.4 | 9.5 | 4.2 | 7.5 | 11.5 | 5.3 | 7.9 | 15 | 15 | 15 |
| y8 (1+) | 9.5 | 3.3 | 2.7 | 9.3 | 5 | 6.5 | 13.3 | 6 | 7 | 15 | 15 | 15 |
| y7 (1+) | 5.8 | 3.2 | 2.8 | 8.4 | 4.6 | 7.5 | 10.2 | 5.6 | 8 | 15 | 15 | 15 |
| sum | 4.6 | 2.8 | 2 | 6.3 | 4.2 | 7.1 | 7.8 | 5 | 7.4 | 15 | 15 | 15 |
Additional Resources and Comments
Comments
Assay Details for CPTAC-1552 Collapse assay details
Data source: Panorama
Official Gene Symbol
HRAS
Peptide Sequence
SFEDIHQYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
89
Peptide End
97
CPTAC ID
CPTAC-1552
Peptide Molecular Mass
1,193.5465
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectSRM2
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 12.5 | 8.2 | 5.8 | 12.7 | 6.9 | 9.4 | 17.8 | 10.7 | 11 | 15 | 15 | 15 |
| y5 (1+) | 28.1 | 5.9 | 7.3 | 24.4 | 8.6 | 8.7 | 37.2 | 10.4 | 11.4 | 15 | 15 | 15 |
| y4 (1+) | 10.6 | 4.4 | 3 | 13.1 | 4.7 | 4.9 | 16.9 | 6.4 | 5.7 | 15 | 15 | 15 |
| a2 (1+) | 6 | 4.5 | 3.7 | 11.2 | 9.6 | 10.1 | 12.7 | 10.6 | 10.8 | 15 | 15 | 15 |
| sum | 3.7 | 3.7 | 2.9 | 9 | 7.5 | 8.3 | 9.7 | 8.4 | 8.8 | 15 | 15 | 15 |