MCM2, CPTAC-1618 - CPTAC Assay Portal (original) (raw)
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MCM2
Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| MCM2 | BM28, CCNL1, CDCL1, KIAA0030 |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 904 | 101896 |
| Protein Name |
|---|
| DNA replication licensing factor MCM2 |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MAESSESFTM | ASSPAQRRRG | NDPLTSSPGR | SSRRTDALTS | SPGRDLPPFE |
| 60 | 70 | 80 | 90 | 100 |
| DESEGLLGTE | GPLEEEEDGE | ELIGDGMERD | YRAIPELDAY | EAEGLALDDE |
| 110 | 120 | 130 | 140 | 150 |
| DVEELTASQR | EAAERAMRQR | DREAGRGLGR | MRRGLLYDSD | EEDEERPARK |
| 160 | 170 | 180 | 190 | 200 |
| RRQVERATED | GEEDEEMIES | IENLEDLKGH | SVREWVSMAG | PRLEIHHRFK |
| 210 | 220 | 230 | 240 | 250 |
| NFLRTHVDSH | GHNVFKERIS | DMCKENRESL | VVNYEDLAAR | EHVLAYFLPE |
| 260 | 270 | 280 | 290 | 300 |
| APAELLQIFD | EAALEVVLAM | YPKYDRITNH | IHVRISHLPL | VEELRSLRQL |
| 310 | 320 | 330 | 340 | 350 |
| HLNQLIRTSG | VVTSCTGVLP | QLSMVKYNCN | KCNFVLGPFC | QSQNQEVKPG |
| 360 | 370 | 380 | 390 | 400 |
| SCPECQSAGP | FEVNMEETIY | QNYQRIRIQE | SPGKVAAGRL | PRSKDAILLA |
| 410 | 420 | 430 | 440 | 450 |
| DLVDSCKPGD | EIELTGIYHN | NYDGSLNTAN | GFPVFATVIL | ANHVAKKDNK |
| 460 | 470 | 480 | 490 | 500 |
| VAVGELTDED | VKMITSLSKD | QQIGEKIFAS | IAPSIYGHED | IKRGLALALF |
| 510 | 520 | 530 | 540 | 550 |
| GGEPKNPGGK | HKVRGDINVL | LCGDPGTAKS | QFLKYIEKVS | SRAIFTTGQG |
| 560 | 570 | 580 | 590 | 600 |
| ASAVGLTAYV | QRHPVSREWT | LEAGALVLAD | RGVCLIDEFD | KMNDQDRTSI |
| 610 | 620 | 630 | 640 | 650 |
| HEAMEQQSIS | ISKAGIVTSL | QARCTVIAAA | NPIGGRYDPS | LTFSENVDLT |
| 660 | 670 | 680 | 690 | 700 |
| EPIISRFDIL | CVVRDTVDPV | QDEMLARFVV | GSHVRHHPSN | KEEEGLANGS |
| 710 | 720 | 730 | 740 | 750 |
| AAEPAMPNTY | GVEPLPQEVL | KKYIIYAKER | VHPKLNQMDQ | DKVAKMYSDL |
| 760 | 770 | 780 | 790 | 800 |
| RKESMATGSI | PITVRHIESM | IRMAEAHARI | HLRDYVIEDD | VNMAIRVMLE |
| 810 | 820 | 830 | 840 | 850 |
| SFIDTQKFSV | MRSMRKTFAR | YLSFRRDNNE | LLLFILKQLV | AEQVTYQRNR |
| 860 | 870 | 880 | 890 | 900 |
| FGAQQDTIEV | PEKDLVDKAR | QINIHNLSAF | YDSELFRMNK | FSHDLKRKMI |
| 904 | ||||
| LQQF |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-1618 Collapse assay details
Data source: Panorama
Official Gene Symbol
MCM2
Peptide Sequence
GLALALFGGEPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
494
Peptide End
505
CPTAC ID
CPTAC-1618
Peptide Molecular Mass
1,171.6601
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectSRM3
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 10.2 | 4.1 | 2.5 | 16.3 | 5.5 | 4.4 | 19.2 | 6.9 | 5.1 | 15 | 15 | 15 |
| y7 (1+) | 10.6 | 1.8 | 2.1 | 13.1 | 5.5 | 3.3 | 16.9 | 5.8 | 3.9 | 15 | 15 | 15 |
| y8 (1+) | 6.5 | 1.8 | 2.6 | 15.4 | 5.5 | 3.4 | 16.7 | 5.8 | 4.3 | 15 | 15 | 15 |
| sum | 4.5 | 1.8 | 1.4 | 12.4 | 5.3 | 2.5 | 13.2 | 5.6 | 2.9 | 15 | 15 | 15 |