PIK3CA, CPTAC-1631 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| PIK3CA | N/A |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 1068 | 124284 |
| Protein Name |
|---|
| Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
CPTAC-1631: View additional LINLTDILK data
CPTAC-3127: View additional YEQYLDNLLVR data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MPPRPSSGEL | WGIHLMPPRI | LVECLLPNGM | IVTLECLREA | TLITIKHELF |
| 60 | 70 | 80 | 90 | 100 |
| KEARKYPLHQ | LLQDESSYIF | VSVTQEAERE | EFFDETRRLC | DLRLFQPFLK |
| 110 | 120 | 130 | 140 | 150 |
| VIEPVGNREE | KILNREIGFA | IGMPVCEFDM | VKDPEVQDFR | RNILNVCKEA |
| 160 | 170 | 180 | 190 | 200 |
| VDLRDLNSPH | SRAMYVYPPN | VESSPELPKH | IYNKLDKGQI | IVVIWVIVSP |
| 210 | 220 | 230 | 240 | 250 |
| NNDKQKYTLK | INHDCVPEQV | IAEAIRKKTR | SMLLSSEQLK | LCVLEYQGKY |
| 260 | 270 | 280 | 290 | 300 |
| ILKVCGCDEY | FLEKYPLSQY | KYIRSCIMLG | RMPNLMLMAK | ESLYSQLPMD |
| 310 | 320 | 330 | 340 | 350 |
| CFTMPSYSRR | ISTATPYMNG | ETSTKSLWVI | NSALRIKILC | ATYVNVNIRD |
| 360 | 370 | 380 | 390 | 400 |
| IDKIYVRTGI | YHGGEPLCDN | VNTQRVPCSN | PRWNEWLNYD | IYIPDLPRAA |
| 410 | 420 | 430 | 440 | 450 |
| RLCLSICSVK | GRKGAKEEHC | PLAWGNINLF | DYTDTLVSGK | MALNLWPVPH |
| 460 | 470 | 480 | 490 | 500 |
| GLEDLLNPIG | VTGSNPNKET | PCLELEFDWF | SSVVKFPDMS | VIEEHANWSV |
| 510 | 520 | 530 | 540 | 550 |
| SREAGFSYSH | AGLSNRLARD | NELRENDKEQ | LKAISTRDPL | SEITEQEKDF |
| 560 | 570 | 580 | 590 | 600 |
| LWSHRHYCVT | IPEILPKLLL | SVKWNSRDEV | AQMYCLVKDW | PPIKPEQAME |
| 610 | 620 | 630 | 640 | 650 |
| LLDCNYPDPM | VRGFAVRCLE | KYLTDDKLSQ | YLIQLVQVLK | YEQYLDNLLV |
| 660 | 670 | 680 | 690 | 700 |
| RFLLKKALTN | QRIGHFFFWH | LKSEMHNKTV | SQRFGLLLES | YCRACGMYLK |
| 710 | 720 | 730 | 740 | 750 |
| HLNRQVEAME | KLINLTDILK | QEKKDETQKV | QMKFLVEQMR | RPDFMDALQG |
| 760 | 770 | 780 | 790 | 800 |
| FLSPLNPAHQ | LGNLRLEECR | IMSSAKRPLW | LNWENPDIMS | ELLFQNNEII |
| 810 | 820 | 830 | 840 | 850 |
| FKNGDDLRQD | MLTLQIIRIM | ENIWQNQGLD | LRMLPYGCLS | IGDCVGLIEV |
| 860 | 870 | 880 | 890 | 900 |
| VRNSHTIMQI | QCKGGLKGAL | QFNSHTLHQW | LKDKNKGEIY | DAAIDLFTRS |
| 910 | 920 | 930 | 940 | 950 |
| CAGYCVATFI | LGIGDRHNSN | IMVKDDGQLF | HIDFGHFLDH | KKKKFGYKRE |
| 960 | 970 | 980 | 990 | 1000 |
| RVPFVLTQDF | LIVISKGAQE | CTKTREFERF | QEMCYKAYLA | IRQHANLFIN |
| 1010 | 1020 | 1030 | 1040 | 1050 |
| LFSMMLGSGM | PELQSFDDIA | YIRKTLALDK | TEQEALEYFM | KQMNDAHHGG |
| 1060 | 1068 | |||
| WTTKMDWIFH | TIKQHALN |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
Click a point on a node
to view detailed assay information below
All other points link out to UniProt
Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-1631 Collapse assay details
Data source: Panorama
Official Gene Symbol
PIK3CA
Peptide Sequence
LINLTDILK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
712
Peptide End
720
CPTAC ID
CPTAC-1631
Peptide Molecular Mass
1,041.6434
Species
Homo sapiens (Human)
Assay Type
Direct MRM or SRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectSRM3
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 17 | 2.9 | 6.6 | 20.8 | 4.5 | 12.6 | 26.9 | 5.4 | 14.2 | 15 | 15 | 15 |
| y6 (1+) | 11.2 | 2.4 | 5.7 | 17.1 | 6.2 | 11 | 20.4 | 6.6 | 12.4 | 15 | 15 | 15 |
| y7 (1+) | 4.9 | 1.1 | 3.5 | 16.7 | 4.6 | 9.1 | 17.4 | 4.7 | 9.7 | 15 | 15 | 15 |
| sum | 4.3 | 1 | 3.5 | 13.5 | 4.5 | 9.4 | 14.2 | 4.6 | 10 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-3129 Collapse assay details
Data source: Panorama
Official Gene Symbol
PIK3CA
Peptide Modified Sequence
SC[+57.0]AGYC[+57.0]VATFILGIGDR
Modification Type
Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification
902, 906
Peptide - Site of Modification
2, 6
Peptide Start
900
Peptide End
916
CPTAC ID
CPTAC-3129
Peptide Molecular Mass
1,858.8706
Species
Homo Sapiens
Assay Type
Direct MRM or SRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectSRM3
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 12.6 | 3.4 | 4.7 | 15.1 | 6.8 | 8.8 | 19.7 | 7.6 | 10 | 15 | 15 | 15 |
| y7 (1+) | 16.4 | 1.9 | 4.3 | 19.2 | 8.9 | 4.8 | 25.3 | 9.1 | 6.4 | 15 | 15 | 15 |
| y6 (1+) | 12.8 | 3 | 5.3 | 14.4 | 7.1 | 8.6 | 19.3 | 7.7 | 10.1 | 15 | 15 | 15 |
| sum | 13 | 2.7 | 4.6 | 15.3 | 6.3 | 7.6 | 20.1 | 6.9 | 8.9 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-3128 Collapse assay details
Data source: Panorama
Official Gene Symbol
PIK3CA
Peptide Modified Sequence
TGIYHGGEPLC[+57.0]DNVNTQR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
369
Peptide - Site of Modification
11
Peptide Start
358
Peptide End
375
CPTAC ID
CPTAC-3128
Peptide Molecular Mass
2,029.9276
Species
Homo Sapiens
Assay Type
Direct MRM or SRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectSRM3
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 4.1 | 2.1 | 3.1 | 7.8 | 2.1 | 3.2 | 8.8 | 3 | 4.5 | 15 | 15 | 15 |
| y8 (1+) | 3.7 | 2.3 | 3.6 | 6.8 | 2.2 | 4.2 | 7.7 | 3.2 | 5.5 | 15 | 15 | 15 |
| b8 (1+) | 5 | 1.7 | 3.3 | 7.9 | 2 | 4 | 9.3 | 2.6 | 5.2 | 15 | 15 | 15 |
| sum | 3.9 | 1.6 | 2.9 | 7.3 | 1.8 | 3.5 | 8.3 | 2.4 | 4.5 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-3127 Collapse assay details
Data source: Panorama
Official Gene Symbol
PIK3CA
Peptide Sequence
YEQYLDNLLVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
641
Peptide End
651
CPTAC ID
CPTAC-3127
Peptide Molecular Mass
1,424.7300
Species
Homo Sapiens
Assay Type
Direct MRM or SRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectSRM3
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 2.9 | 3.8 | 5.5 | 4.7 | 5.6 | 4.8 | 5.5 | 6.8 | 7.3 | 15 | 15 | 15 |
| y8 (1+) | 2.7 | 4.9 | 5.2 | 4.4 | 5.9 | 4.9 | 5.2 | 7.7 | 7.1 | 15 | 15 | 15 |
| y7 (1+) | 1.9 | 4.2 | 4.9 | 2.2 | 5.9 | 4.5 | 2.9 | 7.2 | 6.7 | 15 | 15 | 15 |
| sum | 1.7 | 4.2 | 5.1 | 3.3 | 5.7 | 4.6 | 3.7 | 7.1 | 6.9 | 15 | 15 | 15 |