CTNNA2, CPTAC-1756 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MTSATSPIIL | KWDPKSLEIR | TLTVERLLEP | LVTQVTTLVN | TSNKGPSGKK |
| 60 | 70 | 80 | 90 | 100 |
| KGRSKKAHVL | AASVEQATQN | FLEKGEQIAK | ESQDLKEELV | AAVEDVRKQG |
| 110 | 120 | 130 | 140 | 150 |
| ETMRIASSEF | ADDPCSSVKR | GTMVRAARAL | LSAVTRLLIL | ADMADVMRLL |
| 160 | 170 | 180 | 190 | 200 |
| SHLKIVEEAL | EAVKNATNEQ | DLANRFKEFG | KEMVKLNYVA | ARRQQELKDP |
| 210 | 220 | 230 | 240 | 250 |
| HCRDEMAAAR | GALKKNATML | YTASQAFLRH | PDVAATRANR | DYVFKQVQEA |
| 260 | 270 | 280 | 290 | 300 |
| IAGISNAAQA | TSPTDEAKGH | TGIGELAAAL | NEFDNKIILD | PMTFSEARFR |
| 310 | 320 | 330 | 340 | 350 |
| PSLEERLESI | ISGAALMADS | SCTRDDRRER | IVAECNAVRQ | ALQDLLSEYM |
| 360 | 370 | 380 | 390 | 400 |
| NNTGRKEKGD | PLNIAIDKMT | KKTRDLRRQL | RKAVMDHISD | SFLETNVPLL |
| 410 | 420 | 430 | 440 | 450 |
| VLIEAAKSGN | EKEVKEYAQV | FREHANKLVE | VANLACSISN | NEEGVKLVRM |
| 460 | 470 | 480 | 490 | 500 |
| AATQIDSLCP | QVINAALTLA | ARPQSKVAQD | NMDVFKDQWE | KQVRVLTEAV |
| 510 | 520 | 530 | 540 | 550 |
| DDITSVDDFL | SVSENHILED | VNKCVIALQE | GDVDTLDRTA | GAIRGRAARV |
| 560 | 570 | 580 | 590 | 600 |
| IHIINAEMEN | YEAGVYTEKV | LEATKLLSET | VMPRFAEQVE | VAIEALSANV |
| 610 | 620 | 630 | 640 | 650 |
| PQPFEENEFI | DASRLVYDGV | RDIRKAVLMI | RTPEELEDDS | DFEQEDYDVR |
| 660 | 670 | 680 | 690 | 700 |
| SRTSVQTEDD | QLIAGQSARA | IMAQLPQEEK | AKIAEQVEIF | HQEKSKLDAE |
| 710 | 720 | 730 | 740 | 750 |
| VAKWDDSGND | IIVLAKQMCM | IMMEMTDFTR | GKGPLKNTSD | VINAAKKIAE |
| 760 | 770 | 780 | 790 | 800 |
| AGSRMDKLAR | AVADQCPDSA | CKQDLLAYLQ | RIALYCHQLN | ICSKVKAEVQ |
| 810 | 820 | 830 | 840 | 850 |
| NLGGELIVSG | TGVQSTFTTF | YEVDCDVIDG | GRASQLSTHL | PTCAEGAPIG |
| 860 | 870 | 880 | 890 | 900 |
| SGSSDSSMLD | SATSLIQAAK | NLMNAVVLTV | KASYVASTKY | QKVYGTAAVN |
| 910 | 920 | 930 | 940 | 950 |
| SPVVSWKMKA | PEKKPLVKRE | KPEEFQTRVR | RGSQKKHISP | VQALSEFKAM |
| 953 | ||||
| DSF |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-1756 Collapse assay details
Data source: Panorama
Official Gene Symbol
CTNNA2
Peptide Modified Sequence
IASSEFADDPC[+57.0]SSVK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
115
Peptide - Site of Modification
11
Peptide Start
105
Peptide End
119
CPTAC ID
CPTAC-1756
Peptide Molecular Mass
1,611.7087
Species
Homo sapiens (Human)
Assay Type
Fractionation PRISM-SRM
Fractionation Approach
high-pressure, high-resolution, high pH reverse phase frationation with online monitoring (PRISM)
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 (uL/min)
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-PRISMSRM
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 4.3 | 4 | 2.3 | 4.9 | 8.3 | 3.3 | 6.5 | 9.2 | 4 | 15 | 15 | 15 |
| y7 (1+) | 5.7 | 5.9 | 2.1 | 5.1 | 6.3 | 2.7 | 7.6 | 8.6 | 3.4 | 15 | 15 | 15 |
| y8 (1+) | 5.6 | 2.5 | 2.4 | 5.1 | 4.1 | 2.6 | 7.6 | 4.8 | 3.5 | 15 | 15 | 15 |
| sum | 3.9 | 3.5 | 1.3 | 3.2 | 6.6 | 1.8 | 5 | 7.5 | 2.2 | 15 | 15 | 15 |