FES, CPTAC-1778 - CPTAC Assay Portal (original) (raw)

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGFSSELCSP QGHGVLQQMQ EAELRLLEGM RKWMAQRVKS DREYAGLLHH
60 70 80 90 100
MSLQDSGGQS RAISPDSPIS QSWAEITSQT EGLSRLLRQH AEDLNSGPLS
110 120 130 140 150
KLSLLIRERQ QLRKTYSEQW QQLQQELTKT HSQDIEKLKS QYRALARDSA
160 170 180 190 200
QAKRKYQEAS KDKDRDKAKD KYVRSLWKLF AHHNRYVLGV RAAQLHHQHH
210 220 230 240 250
HQLLLPGLLR SLQDLHEEMA CILKEILQEY LEISSLVQDE VVAIHREMAA
260 270 280 290 300
AAARIQPEAE YQGFLRQYGS APDVPPCVTF DESLLEEGEP LEPGELQLNE
310 320 330 340 350
LTVESVQHTL TSVTDELAVA TEMVFRRQEM VTQLQQELRN EEENTHPRER
360 370 380 390 400
VQLLGKRQVL QEALQGLQVA LCSQAKLQAQ QELLQTKLEH LGPGEPPPVL
410 420 430 440 450
LLQDDRHSTS SSEQEREGGR TPTLEILKSH ISGIFRPKFS LPPPLQLIPE
460 470 480 490 500
VQKPLHEQLW YHGAIPRAEV AELLVHSGDF LVRESQGKQE YVLSVLWDGL
510 520 530 540 550
PRHFIIQSLD NLYRLEGEGF PSIPLLIDHL LSTQQPLTKK SGVVLHRAVP
560 570 580 590 600
KDKWVLNHED LVLGEQIGRG NFGEVFSGRL RADNTLVAVK SCRETLPPDL
610 620 630 640 650
KAKFLQEARI LKQYSHPNIV RLIGVCTQKQ PIYIVMELVQ GGDFLTFLRT
660 670 680 690 700
EGARLRVKTL LQMVGDAAAG MEYLESKCCI HRDLAARNCL VTEKNVLKIS
710 720 730 740 750
DFGMSREEAD GVYAASGGLR QVPVKWTAPE ALNYGRYSSE SDVWSFGILL
760 770 780 790 800
WETFSLGASP YPNLSNQQTR EFVEKGGRLP CPELCPDAVF RLMEQCWAYE
810 820 822
PGQRPSFSTI YQELQSIRKR HR

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1778 Collapse assay details

Data source: Panorama

Official Gene Symbol

FES

Peptide Modified Sequence

EEADGVY[+80.0]AASGGLR

Modification Type

Phospho (Y)

Protein - Site of Modification

713

Peptide - Site of Modification

7

Peptide Start

707

Peptide End

720

CPTAC ID

CPTAC-1778

Peptide Molecular Mass

1,473.6137

Species

Homo sapiens (Human)

Assay Type

Direct PRM

Matrix

E.coli Lysate

Submitting Laboratory

Johns Hopkins University / Pandey

Submitting Lab PI

Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Orbitrap Fusion Lumos

Internal Standard

100fmol

Peptide Standard Purity

>80%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

Easy nLC 1200 System

Column Packing

C18 2um 100A

Column Dimensions

75um i.d. x 50cm

Flow Rate

300nl/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y5 (1+) | 5.5 | 8.1 | 9.7 | 7.5 | 9.8 | 11.1 | 9.3 | 12.7 | 14.7 | 15 | 15 | 15 | | y6 (1+) | 6.5 | 8.2 | 9 | 6.9 | 9.8 | 10.4 | 9.5 | 12.8 | 13.8 | 15 | 15 | 15 | | y7 (1+) | 4.8 | 8.3 | 9.3 | 7.6 | 9.6 | 10.7 | 9 | 12.7 | 14.2 | 15 | 15 | 15 | | y8 (1+) | 5.1 | 7.2 | 7.5 | 6.4 | 8.3 | 9 | 8.2 | 11 | 11.7 | 15 | 15 | 15 | | y10 (1+) | 5.5 | 8.8 | 8.2 | 7.2 | 9.3 | 9.5 | 9.1 | 12.8 | 12.5 | 15 | 15 | 15 | | y11 (1+) | 5.9 | 6.5 | 8.7 | 5.9 | 7.9 | 8.9 | 8.3 | 10.2 | 12.4 | 15 | 15 | 15 | | sum | 4.8 | 7.6 | 8.2 | 6.6 | 8.8 | 9.4 | 8.2 | 11.6 | 12.5 | 15 | 15 | 15 |

Additional Resources and Comments