TNK2, CPTAC-1779 - CPTAC Assay Portal (original) (raw)

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQPEEGTGWL LELLSEVQLQ QYFLRLRDDL NVTRLSHFEY VKNEDLEKIG
60 70 80 90 100
MGRPGQRRLW EAVKRRKALC KRKSWMSKVF SGKRLEAEFP PHHSQSTFRK
110 120 130 140 150
TSPAPGGPAG EGPLQSLTCL IGEKDLRLLE KLGDGSFGVV RRGEWDAPSG
160 170 180 190 200
KTVSVAVKCL KPDVLSQPEA MDDFIREVNA MHSLDHRNLI RLYGVVLTPP
210 220 230 240 250
MKMVTELAPL GSLLDRLRKH QGHFLLGTLS RYAVQVAEGM GYLESKRFIH
260 270 280 290 300
RDLAARNLLL ATRDLVKIGD FGLMRALPQN DDHYVMQEHR KVPFAWCAPE
310 320 330 340 350
SLKTRTFSHA SDTWMFGVTL WEMFTYGQEP WIGLNGSQIL HKIDKEGERL
360 370 380 390 400
PRPEDCPQDI YNVMVQCWAH KPEDRPTFVA LRDFLLEAQP TDMRALQDFE
410 420 430 440 450
EPDKLHIQMN DVITVIEGRA ENYWWRGQNT RTLCVGPFPR NVVTSVAGLS
460 470 480 490 500
AQDISQPLQN SFIHTGHGDS DPRHCWGFPD RIDELYLGNP MDPPDLLSVE
510 520 530 540 550
LSTSRPPQHL GGVKKPTYDP VSEDQDPLSS DFKRLGLRKP GLPRGLWLAK
560 570 580 590 600
PSARVPGTKA SRGSGAEVTL IDFGEEPVVP ALRPCAPSLA QLAMDACSLL
610 620 630 640 650
DETPPQSPTR ALPRPLHPTP VVDWDARPLP PPPAYDDVAQ DEDDFEICSI
660 670 680 690 700
NSTLVGAGVP AGPSQGQTNY AFVPEQARPP PPLEDNLFLP PQGGGKPPSS
710 720 730 740 750
AQTAEIFQAL QQECMRQLQA PAGSPAPSPS PGGDDKPQVP PRVPIPPRPT
760 770 780 790 800
RPHVQLSPAP PGEEETSQWP GPASPPRVPP REPLSPQGSR TPSPLVPPGS
810 820 830 840 850
SPLPPRLSSS PGKTMPTTQS FASDPKYATP QVIQAPGPRA GPCILPIVRD
860 870 880 890 900
GKKVSSTHYY LLPERPSYLE RYQRFLREAQ SPEEPTPLPV PLLLPPPSTP
910 920 930 940 950
APAAPTATVR PMPQAALDPK ANFSTNNSNP GARPPPPRAT ARLPQRGCPG
960 970 980 990 1000
DGPEAGRPAD KIQMAMVHGV TTEECQAALQ CHGWSVQRAA QYLKVEQLFG
1010 1020 1030 1038
LGLRPRGECH KVLEMFDWNL EQAGCHLLGS WGPAHHKR

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1779 Collapse assay details

Data source: Panorama

Official Gene Symbol

TNK2

Peptide Modified Sequence

ALPQNDDHY[+80.0]VMQEHR

Modification Type

Phospho (Y)

Protein - Site of Modification

284

Peptide - Site of Modification

9

Peptide Start

276

Peptide End

290

CPTAC ID

CPTAC-1779

Peptide Molecular Mass

1,931.7986

Species

Homo sapiens (Human)

Assay Type

Direct PRM

Matrix

E.coli Lysate

Submitting Laboratory

Johns Hopkins University / Pandey

Submitting Lab PI

Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Orbitrap Fusion Lumos

Internal Standard

100fmol

Peptide Standard Purity

>80%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

Easy nLC 1200 System

Column Packing

C18 2um 100A

Column Dimensions

75um i.d. x 50cm

Flow Rate

300nl/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y9 (1+) | 71.2 | 19 | 13 | 77 | 24.3 | 12.8 | 104.9 | 30.8 | 18.2 | 15 | 15 | 15 | | y8 (1+) | 38.8 | 12 | 9.1 | 46.3 | 13.2 | 8.3 | 60.4 | 17.8 | 12.3 | 15 | 15 | 15 | | y7 (1+) | 85.5 | 10.9 | 6.8 | 127.7 | 16.1 | 7.6 | 153.7 | 19.4 | 10.2 | 15 | 15 | 15 | | y6 (1+) | 84.1 | 11 | 5.5 | 91.9 | 18.5 | 6.7 | 124.6 | 21.5 | 8.7 | 15 | 15 | 15 | | y5 (1+) | 62.7 | 7.2 | 7.5 | 62.8 | 10.5 | 6.9 | 88.7 | 12.7 | 10.2 | 15 | 15 | 15 | | sum | 29.1 | 9 | 6 | 48.9 | 12 | 5.2 | 56.9 | 15 | 7.9 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-2789 Collapse assay details

Data source: Panorama

Official Gene Symbol

TNK2

Peptide Modified Sequence

Y[+80.0]ATPQVIQAPGPR

Modification Type

Phospho (Y)

Protein - Site of Modification

N/A

Peptide - Site of Modification

1

Peptide Start

827

Peptide End

839

CPTAC ID

CPTAC-2789

Peptide Molecular Mass

1,476.7126

Species

Homo sapiens (Human)

Assay Type

Direct PRM

Matrix

Mammalian Cell Lysate

Submitting Laboratory

Johns Hopkins University / Pandey

Submitting Lab PI

Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

Orbitrap Fusion Lumos

Internal Standard

100fmol

Peptide Standard Purity

Crude (~60%)

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

Easy nLC 1200 System

Column Packing

C18 2um 100A

Column Dimensions

75um i.d. x 50cm

Flow Rate

250nl/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y10 (1+) | 6.7 | 4.5 | 5.4 | 14.5 | 7.4 | 5.1 | 16 | 8.7 | 7.4 | 15 | 15 | 15 | | y8 (1+) | 6.6 | 4.2 | 5.8 | 14.3 | 6.6 | 5.8 | 15.7 | 7.8 | 8.2 | 15 | 15 | 15 | | y7 (1+) | 5.5 | 4.4 | 5.4 | 14.1 | 6.2 | 5.4 | 15.1 | 7.6 | 7.6 | 15 | 15 | 15 | | y6 (1+) | 5.9 | 3 | 5.4 | 13.5 | 5.8 | 5.2 | 14.7 | 6.5 | 7.5 | 15 | 15 | 15 | | sum | 6 | 4 | 5.4 | 14.1 | 6.5 | 5.3 | 15.3 | 7.6 | 7.6 | 15 | 15 | 15 |

Additional Resources and Comments