ICAM1, CPTAC-219 - CPTAC Assay Portal (original) (raw)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
non-CPTAC-3362: View additional GEEILSR data
non-CPTAC-2679: View additional LLGIETPLPK data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MAPSSPRPAL | PALLVLLGAL | FPGPGNAQTS | VSPSKVILPR | GGSVLVTCST |
| 60 | 70 | 80 | 90 | 100 |
| SCDQPKLLGI | ETPLPKKELL | LPGNNRKVYE | LSNVQEDSQP | MCYSNCPDGQ |
| 110 | 120 | 130 | 140 | 150 |
| STAKTFLTVY | WTPERVELAP | LPSWQPVGKN | LTLRCQVEGG | APRANLTVVL |
| 160 | 170 | 180 | 190 | 200 |
| LRGEKELKRE | PAVGEPAEVT | TTVLVRRDHH | GANFSCRTEL | DLRPQGLELF |
| 210 | 220 | 230 | 240 | 250 |
| ENTSAPYQLQ | TFVLPATPPQ | LVSPRVLEVD | TQGTVVCSLD | GLFPVSEAQV |
| 260 | 270 | 280 | 290 | 300 |
| HLALGDQRLN | PTVTYGNDSF | SAKASVSVTA | EDEGTQRLTC | AVILGNQSQE |
| 310 | 320 | 330 | 340 | 350 |
| TLQTVTIYSF | PAPNVILTKP | EVSEGTEVTV | KCEAHPRAKV | TLNGVPAQPL |
| 360 | 370 | 380 | 390 | 400 |
| GPRAQLLLKA | TPEDNGRSFS | CSATLEVAGQ | LIHKNQTREL | RVLYGPRLDE |
| 410 | 420 | 430 | 440 | 450 |
| RDCPGNWTWP | ENSQQTPMCQ | AWGNPLPELK | CLKDGTFPLP | IGESVTVTRD |
| 460 | 470 | 480 | 490 | 500 |
| LEGTYLCRAR | STQGEVTRKV | TVNVLSPRYE | IVIITVVAAA | VIMGTAGLST |
| 510 | 520 | 530 | 532 | |
| YLYNRQRKIK | KYRLQQAQKG | TPMKPNTQAT | PP |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-5943 Collapse assay details
Data source: Panorama
Official Gene Symbol
ICAM1
Peptide Sequence
DGTFPLPIGESVTVTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
4
Peptide Start
434
Peptide End
449
CPTAC ID
CPTAC-5943
Peptide Molecular Mass
1,687.8781
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
FFPE tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
0.3 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b6 (1+) | 24.8 | 23.5 | 10.4 | 32.5 | 24.1 | 9.7 | 40.9 | 33.7 | 14.2 | 13 | 25 | 14 |
| y11 (1+) | 49.7 | 21.6 | 14.2 | 65.9 | 25.4 | 17 | 82.5 | 33.3 | 22.2 | 13 | 25 | 14 |
| y10 (1+) | 11.5 | 12.6 | 6 | 11.5 | 12.9 | 13.4 | 16.3 | 18 | 14.7 | 13 | 25 | 14 |
| y13 (2+) | 17 | 21.1 | 14.1 | 22 | 23.9 | 14.1 | 27.8 | 31.9 | 19.9 | 13 | 25 | 14 |
| y12 (2+) | 31.2 | 13.5 | 7.8 | 37.4 | 17.1 | 11.4 | 48.7 | 21.8 | 13.8 | 13 | 25 | 14 |
| y10 (2+) | 35.2 | 18.7 | 9.8 | 23.9 | 17.3 | 10.5 | 42.5 | 25.5 | 14.4 | 13 | 25 | 14 |
| sum | 9.2 | 9.5 | 5.1 | 12.5 | 12.1 | 9.5 | 15.5 | 15.4 | 10.8 | 13 | 25 | 14 |
Additional Resources and Comments
Assay Details for CPTAC-219 Collapse assay details
Data source: Panorama
Official Gene Symbol
ICAM1
Peptide Sequence
VELAPLPSWQPVGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
116
Peptide End
129
CPTAC ID
CPTAC-219
Peptide Molecular Mass
1,519.8399
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y11 (1+) | 12.2 | 8.9 | 9.4 | 17.4 | 13.9 | 16.8 | 21.3 | 16.5 | 19.3 | 15 | 15 | 15 |
| y10 (1+) | 7.9 | 6.6 | 5.2 | 15.4 | 8.3 | 9 | 17.3 | 10.6 | 10.4 | 15 | 15 | 15 |
| y8 (1+) | 6 | 4.6 | 6.5 | 16.2 | 7.8 | 10.9 | 17.3 | 9.1 | 12.7 | 15 | 15 | 15 |
| sum | 6.9 | 4.7 | 4.1 | 15.6 | 7.8 | 9.8 | 17.1 | 9.1 | 10.6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-220 Collapse assay details
Data source: Panorama
Official Gene Symbol
ICAM1
Peptide Sequence
VTLNGVPAQPLGPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
340
Peptide End
353
CPTAC ID
CPTAC-220
Peptide Molecular Mass
1,417.8041
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich
Publication
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y11 (1+) | 5.7 | 3.2 | 4.5 | 9.3 | 3.5 | 5.8 | 10.9 | 4.7 | 7.3 | 15 | 15 | 15 |
| y10 (1+) | 5 | 5.4 | 6.7 | 7.4 | 8 | 10.3 | 8.9 | 9.7 | 12.3 | 15 | 15 | 15 |
| y8 (1+) | 3.8 | 2.6 | 3.5 | 6.1 | 5.5 | 11.2 | 7.2 | 6.1 | 11.7 | 15 | 15 | 15 |
| sum | 3.7 | 2.4 | 3.4 | 6.1 | 5.3 | 10.6 | 7.1 | 5.8 | 11.1 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-3362 Collapse assay details
Data source: Panorama
Official Gene Symbol
Icam1
Peptide Sequence
GEEILSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
152
Peptide End
158
CPTAC ID
non-CPTAC-3362
Peptide Molecular Mass
802.4185
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Publication
View Details (opens in a new window)
Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b4 (2+) | 15.8 | 8.4 | 7.2 | 6.9 | 15 | 8.1 | 17.2 | 17.2 | 10.8 | 15 | 15 | 15 |
| b4 (1+) | 12.3 | 6 | 8.1 | 14.4 | 11.1 | 7.7 | 18.9 | 12.6 | 11.2 | 15 | 15 | 15 |
| y3 (1+) | 10.6 | 6.7 | 7.7 | 22.3 | 7.4 | 8.2 | 24.7 | 10 | 11.2 | 15 | 15 | 15 |
| y4 (1+) | 9.4 | 9.2 | 4.1 | 17.9 | 10.1 | 5.4 | 20.2 | 13.7 | 6.8 | 15 | 15 | 15 |
| y5 (1+) | 7.4 | 6.7 | 5.5 | 16.1 | 11.8 | 6.2 | 17.7 | 13.6 | 8.3 | 15 | 15 | 15 |
| sum | 2.3 | 4 | 3.3 | 10.3 | 8.1 | 3.8 | 10.6 | 9 | 5 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-2679 Collapse assay details
Data source: Panorama
Official Gene Symbol
ICAM1
Peptide Sequence
LLGIETPLPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
57
Peptide End
66
CPTAC ID
non-CPTAC-2679
Peptide Molecular Mass
1,079.6590
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (2+) | 21.5 | 9.1 | 6.2 | 25.3 | 10.6 | 9.2 | 33.2 | 14 | 11.1 | 15 | 15 | 15 |
| y8 (1+) | 9.6 | 6.8 | 9.3 | 14 | 5.5 | 8.6 | 17 | 8.7 | 12.7 | 15 | 15 | 15 |
| y6 (1+) | 12.3 | 7.7 | 7 | 16.6 | 9.1 | 9.3 | 20.7 | 11.9 | 11.6 | 15 | 15 | 15 |
| b3 (1+) | 15.9 | 8.4 | 7 | 15 | 10.6 | 9.5 | 21.9 | 13.5 | 11.8 | 15 | 15 | 15 |
| y4 (1+) | 16.7 | 8.2 | 8.6 | 24.6 | 9.7 | 9.9 | 29.7 | 12.7 | 13.1 | 15 | 15 | 15 |
| sum | 6.1 | 4.3 | 7.2 | 11.8 | 5.6 | 7.9 | 13.3 | 7.1 | 10.7 | 15 | 15 | 15 |