KAT5, CPTAC-2605 - CPTAC Assay Portal (original) (raw)

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Protein Sequence hover to view complete sequence

CPTAC-2606: View additional GFHIVGYFSK data

CPTAC-2605: View additional SHDDIVTR data

10 20 30 40 50
MAEVGEIIEG CRLPVLRRNQ DNEDEWPLAE ILSVKDISGR KLFYVHYIDF
60 70 80 90 100
NKRLDEWVTH ERLDLKKIQF PKKEAKTPTK NGLPGSRPGS PEREVPASAQ
110 120 130 140 150
ASGKTLPIPV QITLRFNLPK EREAIPGGEP DQPLSSSSCL QPNHRSTKRK
160 170 180 190 200
VEVVSPATPV PSETAPASVF PQNGAARRAV AAQPGRKRKS NCLGTDEDSQ
210 220 230 240 250
DSSDGIPSAP RMTGSLVSDR SHDDIVTRMK NIECIELGRH RLKPWYFSPY
260 270 280 290 300
PQELTTLPVL YLCEFCLKYG RSLKCLQRHL TKCDLRHPPG NEIYRKGTIS
310 320 330 340 350
FFEIDGRKNK SYSQNLCLLA KCFLDHKTLY YDTDPFLFYV MTEYDCKGFH
360 370 380 390 400
IVGYFSKEKE STEDYNVACI LTLPPYQRRG YGKLLIEFSY ELSKVEGKTG
410 420 430 440 450
TPEKPLSDLG LLSYRSYWSQ TILEILMGLK SESGERPQIT INEISEITSI
460 470 480 490 500
KKEDVISTLQ YLNLINYYKG QYILTLSEDI VDGHERAMLK RLLRIDSKCL
510 513
HFTPKDWSKR GKW

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2606 Collapse assay details

Data source: Panorama

Official Gene Symbol

KAT5

Peptide Sequence

GFHIVGYFSK

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

348

Peptide End

357

CPTAC ID

CPTAC-2606

Peptide Molecular Mass

1,153.5920

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

Human ovarian tumor tissue digest

Submitting Laboratory

Johns Hopkins University

Submitting Lab PI

Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

TSQ Vantage

Internal Standard

Stable isotope-labeled peptides

Peptide Standard Purity

Crude (~60%)

Peptide Standard Label Type

13C and 15N at C-terminus K

LC

Accela 1250 Quaternary Low Pump

Column Packing

C18, 3 µm, 300 Å

Column Dimensions

1.0 mm I.D. x 15 cm

Flow Rate

50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-I

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y5 (1+) | 7.3 | 7.2 | 4.8 | 7.4 | 12.3 | 6.1 | 10.4 | 14.3 | 7.8 | 15 | 15 | 15 | | b4 (1+) | 19.1 | 8.5 | 6.8 | 17.5 | 13.4 | 7.5 | 25.9 | 15.9 | 10.1 | 15 | 15 | 15 | | b6 (1+) | 9.7 | 7.7 | 5.5 | 9.3 | 12.7 | 6.4 | 13.4 | 14.9 | 8.4 | 15 | 15 | 15 | | sum | 5 | 7 | 4.7 | 4.5 | 12.5 | 5.8 | 6.7 | 14.3 | 7.5 | 15 | 15 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-2605 Collapse assay details

Data source: Panorama

Official Gene Symbol

KAT5

Peptide Sequence

SHDDIVTR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

221

Peptide End

228

CPTAC ID

CPTAC-2605

Peptide Molecular Mass

941.4567

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

Human ovarian tumor tissue digest

Submitting Laboratory

Johns Hopkins University

Submitting Lab PI

Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

TSQ Vantage

Internal Standard

Stable isotope-labeled peptides

Peptide Standard Purity

Crude (~60%)

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

Accela 1250 Quaternary Low Pump

Column Packing

C18, 3 µm, 300 Å

Column Dimensions

1.0 mm I.D. x 15 cm

Flow Rate

50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-I

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y6 (1+) | 3.5 | 3.1 | 3.8 | 4.1 | 6.3 | 4.6 | 5.4 | 7 | 6 | 15 | 15 | 15 | | y5 (1+) | 4.2 | 4 | 4.5 | 4.1 | 7.1 | 4.9 | 5.9 | 8.1 | 6.7 | 15 | 15 | 15 | | y4 (1+) | 6.3 | 3.4 | 4.2 | 6.3 | 6.4 | 4.7 | 8.9 | 7.2 | 6.3 | 15 | 15 | 15 | | sum | 2.7 | 3.2 | 3.7 | 3 | 6.3 | 4.4 | 4 | 7.1 | 5.7 | 15 | 15 | 15 |

Additional Resources and Comments