SLC2A1, CPTAC-274 - CPTAC Assay Portal (original) (raw)

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SLC2A1	GLUT1

Sequence Length (AA)	Molecular Weight (Da)
492	54084

Protein Name
Solute carrier family 2, facilitated glucose transporter member 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

CPTAC-275: View additional TFDEIASGFR data

CPTAC-274: View additional VTILELFR data
CPTAC-2736: View additional VTILELFR data

10	20	30	40	50
MEPSSKKLTG	RLMLAVGGAV	LGSLQFGYNT	GVINAPQKVI	EEFYNQTWVH
60	70	80	90	100
RYGESILPTT	LTTLWSLSVA	IFSVGGMIGS	FSVGLFVNRF	GRRNSMLMMN
110	120	130	140	150
LLAFVSAVLM	GFSKLGKSFE	MLILGRFIIG	VYCGLTTGFV	PMYVGEVSPT
160	170	180	190	200
ALRGALGTLH	QLGIVVGILI	AQVFGLDSIM	GNKDLWPLLL	SIIFIPALLQ
210	220	230	240	250
CIVLPFCPES	PRFLLINRNE	ENRAKSVLKK	LRGTADVTHD	LQEMKEESRQ
260	270	280	290	300
MMREKKVTIL	ELFRSPAYRQ	PILIAVVLQL	SQQLSGINAV	FYYSTSIFEK
310	320	330	340	350
AGVQQPVYAT	IGSGIVNTAF	TVVSLFVVER	AGRRTLHLIG	LAGMAGCAIL
360	370	380	390	400
MTIALALLEQ	LPWMSYLSIV	AIFGFVAFFE	VGPGPIPWFI	VAELFSQGPR
410	420	430	440	450
PAAIAVAGFS	NWTSNFIVGM	CFQYVEQLCG	PYVFIIFTVL	LVLFFIFTYF
460	470	480	490	492
KVPETKGRTF	DEIASGFRQG	GASQSDKTPE	ELFHPLGADS	QV

Data source: UniProt

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-275 Collapse assay details

Data source: Panorama

Official Gene Symbol

SLC2A1

Peptide Sequence

TFDEIASGFR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

459

Peptide End

468

CPTAC ID

CPTAC-275

Peptide Molecular Mass

1,141.5404

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

cell line lysate pool

Submitting Laboratory

Fred Hutchinson Cancer Research Center

Submitting Lab PI

Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

5500 QTRAP (ABSCIEX)

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)

Column Packing

ChromXP C18-CL, 3 um, 120A

Column Dimensions

150 x 0.075 mm

Flow Rate

500 nL / min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV equation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y8 (1+) | 6 | 5.4 | 4.5 | 7 | 7.4 | 5.3 | 9.2 | 9.2 | 7 | 15 | 25 | 15 | | y7 (1+) | 7.8 | 9.4 | 6.3 | 12.6 | 12.6 | 9.3 | 14.8 | 15.7 | 11.2 | 15 | 25 | 15 | | y6 (1+) | 12 | 9.6 | 8.6 | 18.1 | 9.1 | 10.1 | 21.7 | 13.2 | 13.3 | 15 | 25 | 15 | | sum | 5.1 | 5.1 | 4.8 | 8.5 | 6.8 | 5.7 | 9.9 | 8.5 | 7.5 | 15 | 25 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-274 Collapse assay details

Data source: Panorama

Official Gene Symbol

SLC2A1

Peptide Sequence

VTILELFR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

257

Peptide End

264

CPTAC ID

CPTAC-274

Peptide Molecular Mass

989.5910

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

cell line lysate pool

Submitting Laboratory

Fred Hutchinson Cancer Research Center

Submitting Lab PI

Amanda Paulovich

Publication

View Details (opens in a new window)

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

5500 QTRAP (ABSCIEX)

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)

Column Packing

ChromXP C18-CL, 3 um, 120A

Column Dimensions

150 x 0.075 mm

Flow Rate

500 nL / min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV equation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y6 (1+) | 6.8 | 6.4 | 4.5 | 16.5 | 16.1 | 10 | 17.8 | 17.3 | 11 | 15 | 25 | 15 | | y5 (1+) | 7.8 | 8.3 | 8.1 | 19.5 | 17.2 | 14.5 | 21 | 19.1 | 16.6 | 15 | 25 | 15 | | y4 (1+) | 6.2 | 9.3 | 7.3 | 16.3 | 18.2 | 11.8 | 17.4 | 20.4 | 13.9 | 15 | 25 | 15 | | sum | 5.5 | 6.9 | 5.7 | 17.2 | 15.7 | 11.9 | 18.1 | 17.1 | 13.2 | 15 | 25 | 15 |

Additional Resources and Comments

Assay Details for CPTAC-2736 Collapse assay details

Data source: Panorama

Official Gene Symbol

SLC2A1

Peptide Sequence

VTILELFR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

257

Peptide End

264

CPTAC ID

CPTAC-2736

Peptide Molecular Mass

989.5910

Species

Homo Sapiens

Assay Type

Direct MRM

Matrix

Tumor Digest

Submitting Laboratory

Washington University in St. Louis

Submitting Lab PI

Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

ThermoFisher Q-Exactive

Internal Standard

13 fmol on column

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

Easy-nLC 1000 Thermo Scientific

Column Packing

C18

Column Dimensions

75 um x 50 cm

Flow Rate

300 nL/min

Chromatograms

Data source: Panorama

Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV equation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y4 (1+) | 6.6 | 3.3 | 6.9 | 6.2 | 3.8 | 7.4 | 9.1 | 5 | 10.1 | 15 | 15 | 15 | | y5 (1+) | 3.4 | 3.1 | 7.4 | 3.2 | 5.1 | 7.4 | 4.7 | 6 | 10.5 | 15 | 15 | 15 | | y6 (1+) | 4.9 | 2.7 | 6.2 | 5 | 4.2 | 7 | 7 | 5 | 9.4 | 15 | 15 | 15 | | sum | 4.2 | 2.8 | 6.5 | 4.2 | 4.3 | 7.1 | 5.9 | 5.1 | 9.6 | 15 | 15 | 15 |