SLC2A1, CPTAC-274 - CPTAC Assay Portal (original) (raw)

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SLC2A1 GLUT1
Sequence Length (AA) Molecular Weight (Da)
492 54084
Protein Name
Solute carrier family 2, facilitated glucose transporter member 1
Sources
UniProt PhosphoSitePlus ® GeneCards Human Protein Atlas

Protein Sequence hover to view complete sequence

CPTAC-275: View additional TFDEIASGFR data

CPTAC-274: View additional VTILELFR data
CPTAC-2736: View additional VTILELFR data

10 20 30 40 50
MEPSSKKLTG RLMLAVGGAV LGSLQFGYNT GVINAPQKVI EEFYNQTWVH
60 70 80 90 100
RYGESILPTT LTTLWSLSVA IFSVGGMIGS FSVGLFVNRF GRRNSMLMMN
110 120 130 140 150
LLAFVSAVLM GFSKLGKSFE MLILGRFIIG VYCGLTTGFV PMYVGEVSPT
160 170 180 190 200
ALRGALGTLH QLGIVVGILI AQVFGLDSIM GNKDLWPLLL SIIFIPALLQ
210 220 230 240 250
CIVLPFCPES PRFLLINRNE ENRAKSVLKK LRGTADVTHD LQEMKEESRQ
260 270 280 290 300
MMREKKVTIL ELFRSPAYRQ PILIAVVLQL SQQLSGINAV FYYSTSIFEK
310 320 330 340 350
AGVQQPVYAT IGSGIVNTAF TVVSLFVVER AGRRTLHLIG LAGMAGCAIL
360 370 380 390 400
MTIALALLEQ LPWMSYLSIV AIFGFVAFFE VGPGPIPWFI VAELFSQGPR
410 420 430 440 450
PAAIAVAGFS NWTSNFIVGM CFQYVEQLCG PYVFIIFTVL LVLFFIFTYF
460 470 480 490 492
KVPETKGRTF DEIASGFRQG GASQSDKTPE ELFHPLGADS QV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-275 Collapse assay details

Data source: Panorama

Official Gene Symbol

SLC2A1

Peptide Sequence

TFDEIASGFR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

459

Peptide End

468

CPTAC ID

CPTAC-275

Peptide Molecular Mass

1,141.5404

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

cell line lysate pool

Submitting Laboratory

Fred Hutchinson Cancer Research Center

Submitting Lab PI

Amanda Paulovich


Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

5500 QTRAP (ABSCIEX)

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)

Column Packing

ChromXP C18-CL, 3 um, 120A

Column Dimensions

150 x 0.075 mm

Flow Rate

500 nL / min


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader


Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y8 (1+) | 6 | 5.4 | 4.5 | 7 | 7.4 | 5.3 | 9.2 | 9.2 | 7 | 15 | 25 | 15 | | y7 (1+) | 7.8 | 9.4 | 6.3 | 12.6 | 12.6 | 9.3 | 14.8 | 15.7 | 11.2 | 15 | 25 | 15 | | y6 (1+) | 12 | 9.6 | 8.6 | 18.1 | 9.1 | 10.1 | 21.7 | 13.2 | 13.3 | 15 | 25 | 15 | | sum | 5.1 | 5.1 | 4.8 | 8.5 | 6.8 | 5.7 | 9.9 | 8.5 | 7.5 | 15 | 25 | 15 |



Additional Resources and Comments


Assay Details for CPTAC-274 Collapse assay details

Data source: Panorama

Official Gene Symbol

SLC2A1

Peptide Sequence

VTILELFR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

257

Peptide End

264

CPTAC ID

CPTAC-274

Peptide Molecular Mass

989.5910

Species

Homo sapiens (Human)

Assay Type

Direct MRM

Matrix

cell line lysate pool

Submitting Laboratory

Fred Hutchinson Cancer Research Center

Submitting Lab PI

Amanda Paulovich


Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

5500 QTRAP (ABSCIEX)

Internal Standard

peptide

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)

Column Packing

ChromXP C18-CL, 3 um, 120A

Column Dimensions

150 x 0.075 mm

Flow Rate

500 nL / min


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader


Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y6 (1+) | 6.8 | 6.4 | 4.5 | 16.5 | 16.1 | 10 | 17.8 | 17.3 | 11 | 15 | 25 | 15 | | y5 (1+) | 7.8 | 8.3 | 8.1 | 19.5 | 17.2 | 14.5 | 21 | 19.1 | 16.6 | 15 | 25 | 15 | | y4 (1+) | 6.2 | 9.3 | 7.3 | 16.3 | 18.2 | 11.8 | 17.4 | 20.4 | 13.9 | 15 | 25 | 15 | | sum | 5.5 | 6.9 | 5.7 | 17.2 | 15.7 | 11.9 | 18.1 | 17.1 | 13.2 | 15 | 25 | 15 |



Additional Resources and Comments


Assay Details for CPTAC-2736 Collapse assay details

Data source: Panorama

Official Gene Symbol

SLC2A1

Peptide Sequence

VTILELFR

Modification Type

unmodified

Protein - Site of Modification

N/A

Peptide - Site of Modification

N/A

Peptide Start

257

Peptide End

264

CPTAC ID

CPTAC-2736

Peptide Molecular Mass

989.5910

Species

Homo Sapiens

Assay Type

Direct MRM

Matrix

Tumor Digest

Submitting Laboratory

Washington University in St. Louis

Submitting Lab PI

Reid Townsend


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument

ThermoFisher Q-Exactive

Internal Standard

13 fmol on column

Peptide Standard Purity

>95%

Peptide Standard Label Type

13C and 15N at C-terminus R

LC

Easy-nLC 1000 Thermo Scientific

Column Packing

C18

Column Dimensions

75 um x 50 cm

Flow Rate

300 nL/min


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader


Repeatability

Data source: Panorama

| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CVequation | n= | | | | | | | | | | | ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | | Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High | | y4 (1+) | 6.6 | 3.3 | 6.9 | 6.2 | 3.8 | 7.4 | 9.1 | 5 | 10.1 | 15 | 15 | 15 | | y5 (1+) | 3.4 | 3.1 | 7.4 | 3.2 | 5.1 | 7.4 | 4.7 | 6 | 10.5 | 15 | 15 | 15 | | y6 (1+) | 4.9 | 2.7 | 6.2 | 5 | 4.2 | 7 | 7 | 5 | 9.4 | 15 | 15 | 15 | | sum | 4.2 | 2.8 | 6.5 | 4.2 | 4.3 | 7.1 | 5.9 | 5.1 | 9.6 | 15 | 15 | 15 |



Additional Resources and Comments