ROR2, CPTAC-2781 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MARGSALPRR | PLLCIPAVWA | AAALLLSVSR | TSGEVEVLDP | NDPLGPLDGQ |
| 60 | 70 | 80 | 90 | 100 |
| DGPIPTLKGY | FLNFLEPVNN | ITIVQGQTAI | LHCKVAGNPP | PNVRWLKNDA |
| 110 | 120 | 130 | 140 | 150 |
| PVVQEPRRII | IRKTEYGSRL | RIQDLDTTDT | GYYQCVATNG | MKTITATGVL |
| 160 | 170 | 180 | 190 | 200 |
| FVRLGPTHSP | NHNFQDDYHE | DGFCQPYRGI | ACARFIGNRT | IYVDSLQMQG |
| 210 | 220 | 230 | 240 | 250 |
| EIENRITAAF | TMIGTSTHLS | DQCSQFAIPS | FCHFVFPLCD | ARSRTPKPRE |
| 260 | 270 | 280 | 290 | 300 |
| LCRDECEVLE | SDLCRQEYTI | ARSNPLILMR | LQLPKCEALP | MPESPDAANC |
| 310 | 320 | 330 | 340 | 350 |
| MRIGIPAERL | GRYHQCYNGS | GMDYRGTAST | TKSGHQCQPW | ALQHPHSHHL |
| 360 | 370 | 380 | 390 | 400 |
| SSTDFPELGG | GHAYCRNPGG | QMEGPWCFTQ | NKNVRMELCD | VPSCSPRDSS |
| 410 | 420 | 430 | 440 | 450 |
| KMGILYILVP | SIAIPLVIAC | LFFLVCMCRN | KQKASASTPQ | RRQLMASPSQ |
| 460 | 470 | 480 | 490 | 500 |
| DMEMPLINQH | KQAKLKEISL | SAVRFMEELG | EDRFGKVYKG | HLFGPAPGEQ |
| 510 | 520 | 530 | 540 | 550 |
| TQAVAIKTLK | DKAEGPLREE | FRHEAMLRAR | LQHPNVVCLL | GVVTKDQPLS |
| 560 | 570 | 580 | 590 | 600 |
| MIFSYCSHGD | LHEFLVMRSP | HSDVGSTDDD | RTVKSALEPP | DFVHLVAQIA |
| 610 | 620 | 630 | 640 | 650 |
| AGMEYLSSHH | VVHKDLATRN | VLVYDKLNVK | ISDLGLFREV | YAADYYKLLG |
| 660 | 670 | 680 | 690 | 700 |
| NSLLPIRWMA | PEAIMYGKFS | IDSDIWSYGV | VLWEVFSYGL | QPYCGYSNQD |
| 710 | 720 | 730 | 740 | 750 |
| VVEMIRNRQV | LPCPDDCPAW | VYALMIECWN | EFPSRRPRFK | DIHSRLRAWG |
| 760 | 770 | 780 | 790 | 800 |
| NLSNYNSSAQ | TSGASNTTQT | SSLSTSPVSN | VSNARYVGPK | QKAPPFPQPQ |
| 810 | 820 | 830 | 840 | 850 |
| FIPMKGQIRP | MVPPPQLYVP | VNGYQPVPAY | GAYLPNFYPV | QIPMQMAPQQ |
| 860 | 870 | 880 | 890 | 900 |
| VPPQMVPKPS | SHHSGSGSTS | TGYVTTAPSN | TSMADRAALL | SEGADDTQNA |
| 910 | 920 | 930 | 940 | 943 |
| PEDGAQSTVQ | EAEEEEEGSV | PETELLGDCD | TLQVDEAQVQ | LEA |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-2781 Collapse assay details
Data source: Panorama
Official Gene Symbol
ROR2
Peptide Modified Sequence
NVLVY[+80.0]DK
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
5
Peptide Start
620
Peptide End
626
CPTAC ID
CPTAC-2781
Peptide Molecular Mass
929.4260
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Mammalian Cell Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
250nl/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 3.1 | 3.3 | 3.6 | 6.6 | 3 | 3.7 | 7.3 | 4.5 | 5.2 | 15 | 15 | 15 |
| y4 (1+) | 2.9 | 3.1 | 3.1 | 6.1 | 2.6 | 3.8 | 6.8 | 4 | 4.9 | 15 | 15 | 15 |
| y3 (1+) | 3.1 | 2.7 | 3.4 | 5.7 | 2.5 | 3.8 | 6.5 | 3.7 | 5.1 | 15 | 15 | 15 |
| y2 (1+) | 4.2 | 2.4 | 2.9 | 6.6 | 2.4 | 3.9 | 7.8 | 3.4 | 4.9 | 15 | 15 | 15 |
| b2 (1+) | 3.6 | 3.1 | 3.5 | 6.7 | 3 | 3.8 | 7.6 | 4.3 | 5.2 | 15 | 15 | 15 |
| b3 (1+) | 6.6 | 4.1 | 3 | 9.5 | 3.5 | 5.7 | 11.6 | 5.4 | 6.4 | 15 | 15 | 15 |
| sum | 2.9 | 2.9 | 3.3 | 6.2 | 2.6 | 3.7 | 6.8 | 3.9 | 5 | 15 | 15 | 15 |