FLT3, CPTAC-2792 - CPTAC Assay Portal (original) (raw)
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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Overview Data source: UniProt
| Official Gene Symbol | Other Aliases |
|---|---|
| FLT3 | CD135, FLK2, STK1 |
| Sequence Length (AA) | Molecular Weight (Da) |
|---|---|
| 993 | 112903 |
| Protein Name |
|---|
| Receptor-type tyrosine-protein kinase FLT3 |
| Sources | |
|---|---|
| UniProt PhosphoSitePlus ® GeneCards | Human Protein Atlas |
Protein Sequence hover to view complete sequence
CPTAC-2823: View additional GLDNGYSISK data
CPTAC-3121: View additional VLHELFGTDIR data
| 10 | 20 | 30 | 40 | 50 |
|---|---|---|---|---|
| MPALARDGGQ | LPLLVVFSAM | IFGTITNQDL | PVIKCVLINH | KNNDSSVGKS |
| 60 | 70 | 80 | 90 | 100 |
| SSYPMVSESP | EDLGCALRPQ | SSGTVYEAAA | VEVDVSASIT | LQVLVDAPGN |
| 110 | 120 | 130 | 140 | 150 |
| ISCLWVFKHS | SLNCQPHFDL | QNRGVVSMVI | LKMTETQAGE | YLLFIQSEAT |
| 160 | 170 | 180 | 190 | 200 |
| NYTILFTVSI | RNTLLYTLRR | PYFRKMENQD | ALVCISESVP | EPIVEWVLCD |
| 210 | 220 | 230 | 240 | 250 |
| SQGESCKEES | PAVVKKEEKV | LHELFGTDIR | CCARNELGRE | CTRLFTIDLN |
| 260 | 270 | 280 | 290 | 300 |
| QTPQTTLPQL | FLKVGEPLWI | RCKAVHVNHG | FGLTWELENK | ALEEGNYFEM |
| 310 | 320 | 330 | 340 | 350 |
| STYSTNRTMI | RILFAFVSSV | ARNDTGYYTC | SSSKHPSQSA | LVTIVEKGFI |
| 360 | 370 | 380 | 390 | 400 |
| NATNSSEDYE | IDQYEEFCFS | VRFKAYPQIR | CTWTFSRKSF | PCEQKGLDNG |
| 410 | 420 | 430 | 440 | 450 |
| YSISKFCNHK | HQPGEYIFHA | ENDDAQFTKM | FTLNIRRKPQ | VLAEASASQA |
| 460 | 470 | 480 | 490 | 500 |
| SCFSDGYPLP | SWTWKKCSDK | SPNCTEEITE | GVWNRKANRK | VFGQWVSSST |
| 510 | 520 | 530 | 540 | 550 |
| LNMSEAIKGF | LVKCCAYNSL | GTSCETILLN | SPGPFPFIQD | NISFYATIGV |
| 560 | 570 | 580 | 590 | 600 |
| CLLFIVVLTL | LICHKYKKQF | RYESQLQMVQ | VTGSSDNEYF | YVDFREYEYD |
| 610 | 620 | 630 | 640 | 650 |
| LKWEFPRENL | EFGKVLGSGA | FGKVMNATAY | GISKTGVSIQ | VAVKMLKEKA |
| 660 | 670 | 680 | 690 | 700 |
| DSSEREALMS | ELKMMTQLGS | HENIVNLLGA | CTLSGPIYLI | FEYCCYGDLL |
| 710 | 720 | 730 | 740 | 750 |
| NYLRSKREKF | HRTWTEIFKE | HNFSFYPTFQ | SHPNSSMPGS | REVQIHPDSD |
| 760 | 770 | 780 | 790 | 800 |
| QISGLHGNSF | HSEDEIEYEN | QKRLEEEEDL | NVLTFEDLLC | FAYQVAKGME |
| 810 | 820 | 830 | 840 | 850 |
| FLEFKSCVHR | DLAARNVLVT | HGKVVKICDF | GLARDIMSDS | NYVVRGNARL |
| 860 | 870 | 880 | 890 | 900 |
| PVKWMAPESL | FEGIYTIKSD | VWSYGILLWE | IFSLGVNPYP | GIPVDANFYK |
| 910 | 920 | 930 | 940 | 950 |
| LIQNGFKMDQ | PFYATEEIYI | IMQSCWAFDS | RKRPSFPNLT | SFLGCQLADA |
| 960 | 970 | 980 | 990 | 993 |
| EEAMYQNVDG | RVSECPHTYQ | NRRPFSREMD | LGLLSPQAQV | EDS |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Phosphorylation Acetylation Ubiquitylation Other
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Assay Details for CPTAC-2792 Collapse assay details
Data source: Panorama
Official Gene Symbol
FLT3
Peptide Modified Sequence
VSEC[+57.0]PHTY[+80.0]QNR
Modification Type
Carbamidomethyl (C), Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
4, 8
Peptide Start
962
Peptide End
972
CPTAC ID
CPTAC-2792
Peptide Molecular Mass
1,469.5759
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Mammalian Cell Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
250nl/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV
| n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 40 | 22 | 12.8 | 44.6 | 41.6 | 18.8 | 59.9 | 47.1 | 22.7 | 15 | 15 | 15 |
| y6 (1+) | 40.4 | 27 | 13.9 | 44.5 | 48.2 | 20.2 | 60.1 | 55.2 | 24.5 | 15 | 15 | 15 |
| y5 (1+) | 41.5 | 29.8 | 15.5 | 50 | 54.5 | 21.2 | 65 | 62.1 | 26.3 | 15 | 15 | 15 |
| y4 (1+) | 38 | 28.5 | 16.9 | 44.8 | 58 | 24.7 | 58.7 | 64.6 | 29.9 | 15 | 15 | 15 |
| y3 (1+) | 38.5 | 30.1 | 21.4 | 59.9 | 64.9 | 27.1 | 71.2 | 71.5 | 34.5 | 15 | 15 | 15 |
| b3 (1+) | 44 | 27.6 | 22.5 | 61.7 | 60 | 31 | 75.8 | 66 | 38.3 | 15 | 15 | 15 |
| sum | 38.9 | 25 | 14.2 | 45 | 48.3 | 20.7 | 59.5 | 54.4 | 25.1 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-2823 Collapse assay details
Data source: Panorama
Official Gene Symbol
FLT3
Peptide Sequence
GLDNGYSISK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
396
Peptide End
405
CPTAC ID
CPTAC-2823
Peptide Molecular Mass
1,052.5138
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive™
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC™ 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nl/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 13.9 | 6.4 | 6.7 | 61.9 | 7.6 | 9.3 | 63.4 | 9.9 | 11.5 | 15 | 15 | 15 |
| y4 (1+) | 6.7 | 4.1 | 6.3 | 5.8 | 6.4 | 5.8 | 8.9 | 7.6 | 8.6 | 15 | 15 | 15 |
| b3 (1+) | 15.1 | 3.7 | 6.8 | 13.4 | 8 | 5.5 | 20.2 | 8.8 | 8.7 | 15 | 15 | 15 |
| sum | 5.2 | 3.6 | 4.9 | 13.5 | 6.4 | 5.5 | 14.5 | 7.3 | 7.4 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-3122 Collapse assay details
Data source: Panorama
Official Gene Symbol
FLT3
Peptide Sequence
HPSQSALVTIVEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
335
Peptide End
347
CPTAC ID
CPTAC-3122
Peptide Molecular Mass
1,407.7722
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 7.8 | 4.9 | 7.3 | 12.1 | 5.5 | 7.7 | 14.4 | 7.4 | 10.6 | 15 | 15 | 15 |
| b6 (1+) | 7.2 | 5.2 | 7.2 | 10.4 | 5.3 | 7.2 | 12.6 | 7.4 | 10.2 | 15 | 15 | 15 |
| y3 (1+) | 7.9 | 4.5 | 8.4 | 11.8 | 5.6 | 9.1 | 14.2 | 7.2 | 12.4 | 15 | 15 | 15 |
| sum | 7.3 | 4.4 | 7.4 | 11.2 | 4.9 | 7.3 | 13.4 | 6.6 | 10.4 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-3121 Collapse assay details
Data source: Panorama
Official Gene Symbol
FLT3
Peptide Sequence
VLHELFGTDIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
220
Peptide End
230
CPTAC ID
CPTAC-3121
Peptide Molecular Mass
1,298.6983
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend
Assay Parameters Collapse assay parameters
Data source: Panorama
Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| | Average intra-assay CV(within day CV) | Average inter-assay CV(between day CV) | Total CV | n= | | | | | | | | | |
| ---------------------------------------- | -------------------------------------- | --------------------------------------------------------------------------------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- | ------- | ------- | -------- |
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b4 (1+) | 6.2 | 6.7 | 2.7 | 15 | 7.3 | 5.8 | 16.2 | 9.9 | 6.4 | 15 | 15 | 15 |
| b3 (1+) | 9 | 6.1 | 3.7 | 13 | 7.5 | 7.6 | 15.8 | 9.7 | 8.5 | 15 | 15 | 15 |
| y5 (1+) | 7.8 | 6.5 | 3.2 | 15.9 | 7.7 | 7 | 17.7 | 10.1 | 7.7 | 15 | 15 | 15 |
| sum | 6.5 | 6.3 | 2.7 | 14.6 | 7.4 | 6.5 | 16 | 9.7 | 7 | 15 | 15 | 15 |